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Nitrous oxide (N₂O), a driver of global warming and climate change, has reached unprecedented concentrations in Earth’s atmosphere. Current N₂O sources outpace N₂O sinks, emphasizing the need for comprehensive understanding of processes that consume N₂O. Microbes that express the enzyme N₂O reductase (N₂OR) convert N₂O to climate change-neutral dinitrogen (N₂). Known N₂ORs belong to the canonical clade I and clade II NosZ reductases and are considered key enzymes for N₂O reduction. Here we report a previously unrecognized protein family with a role in N₂O reduction, clade III lactonase-type N₂OR (L-N₂OR), which diverges in sequence from canonical NosZ but conserves three-dimensionalmore » protein structural features. Integrated physiological, metagenomic, proteomic and structural modelling studies demonstrate that L-N₂ORs catalyse N₂O reduction. L-N₂OR genes occur in several phyla, predominantly in uncultured taxa with broad geographic distribution. Our findings expand the known diversity of N₂ORs and implicate previously unrecognized taxa (for example, Nitrospinota) in N₂O consumption. In conclusion, the expansion of N₂OR diversity and the identification of a novel type of catalyst for N₂O reduction advances the understanding of N₂O sinks, has implications for greenhouse gas emission and climate change modelling, and expands opportunities for innovative biotechnologies aimed at curbing N₂O emissions.« less

Nitrous oxide (N₂O) is a climate-active gas with emissions predicted to increase due to agricultural intensification. Microbial reduction of N₂O to dinitrogen (N₂) is the major consumption process but microbial N₂O reduction under acidic conditions is considered negligible, albeit strongly acidic soils harbor nosZ genes encoding N₂O reductase. Here, we study a co-culture derived from acidic tropical forest soil that reduces N₂O at pH 4.5. The co-culture exhibits bimodal growth with a Serratia sp. fermenting pyruvate followed by hydrogenotrophic N₂O reduction by a Desulfosporosinus sp. Integrated omics and physiological characterization revealed interspecies nutritional interactions, with the pyruvate fermenting Serratia sp.more » supplying amino acids as essential growth factors to the N₂O-reducing Desulfosporosinus sp. Thus, we demonstrate growth-linked N₂O reduction between pH 4.5 and 6, highlighting microbial N₂O reduction potential in acidic soils.« less

Two anaerobic, tetrachloroethene- (PCE-) respiring bacterial isolates, designated strain ACS_DCE ^T and strain ACS_TCE, were characterized using a polyphasic approach. Cells were Gram-stain-negative, motile, non-spore-forming and shared a vibrioid- to spirillum-shaped morphology. Optimum growth occurred at 30°C and 0.1–0.4% salinity. The pH range for growth was pH 5.5–7.5, with an optimum at pH 7.2. Hydrogen, formate, pyruvate and lactate as electron donors supported respiratory reductive dechlorination of PCE to cis-1,2-dichloroethene (cDCE) in strain ACS_DCE ^T and of PCE to trichloroethene (TCE) in strain ACS_TCE. Both strains were able to grow with pyruvate under microaerobic conditions. Nitrate, elemental sulphur, and thiosulphatemore » were alternative electron acceptors. Autotrophic growth was not observed and acetate served as carbon source for both strains. The major cellular fatty acids were C_16:1 ω7c, C_16:0, C_14:0 and C_18:1 ω7c. Both genomes feature a circular plasmid. Strains ACS_DCE ^T and ACS_TCE were previously assigned to the candidate species 'Sulfurospirillum acididehalogenans'. Here, based on key genomic features and pairwise comparisons of whole-genome sequences, including average nucleotide identity, digital DNA–DNA hybridization and average amino acid identity, strains ACS_DCE ^T and ACS_TCE, 'Ca. Sulfurospirillum diekertiae' strains SL2-1 and SL2-2, and the unclassified Sulfurospirillum sp. strain SPD-1 are grouped into one distinct species separate from previously described Sulfurospirillum species. Compared to Sulfurospirillum multivorans and Sulfurospirillum halorespirans, which dechlorinate PCE to cDCE without substantial TCE accumulation, these five strains produce TCE or cDCE as the end product. In addition, some cellular fatty acids (e.g., C_16:0 3OH, C_17:0 iso 3OH, C_17:0 2OH) were detected in strains ACS_DCE ^T and ACS_TCE but not in other Sulfurospirillum species. On the basis of phylogenetic, physiological and phenotypic characteristics, 'Ca. Sulfurospirillum acididehalogenans' and 'Ca. Sulfurospirillum diekertiae' are proposed to be merged into one novel species within the genus Sulfurospirillum, for which the name Sulfurospirillum diekertiae sp. nov. is proposed. Finally, the type strain is ACS_DCE ^T (=JCM 33349^T= KCTC 15819^T=CGMCC 1.5292^T).« less

Bisphenol A (BPA) is a high production volume chemical with potential estrogenic effects susceptible to abiotic degradation by MnO₂. BPA transformation products and reaction mechanisms with MnO₂ have been investigated, but detailed process understanding of Mn(III)-mediated degradation has not been attained. Rapid consumption of BPA occurred in batch reaction vessels with 1 mM Mn(III) and 63.9 ± 0.7% of 1.76 ± 0.02 μmol BPA was degraded in 1 hour at circumneutral pH. BPA was consumed at 1.86 ± 0.09-fold higher rates in vessels with synthetic MnO₂ comprising approximately 13 mol% surface-associated Mn(III) versus surface-Mn(III)-free MnO₂, and 10–35% of BPA transformationmore » could be attributed to Mn(III) during the initial 10-min reaction phase. High-resolution tandem mass spectrometry (HRMS/MS) analysis detected eight transformation intermediates in reactions with Mn(III), and quantum calculations proposed 14 BPA degradation products, nine of which had not been observed during MnO₂-mediated BPA degradation, suggesting mechanistic differences between Mn(III)- versus MnO₂-mediated BPA degradation. Finally, the findings demonstrate that both Mn(III) and Mn(IV) can effectively degrade BPA and indicate that surface-associated Mn(III) increases the reactivity of synthetic MnO₂, offering opportunities for engineering more reactive oxidized Mn species for BPA removal.« less

Isoprene is a ubiquitously distributed, biogenic, and climate-active organic compound. Microbial isoprene degradation in oxic environments is fairly well understood; however, studies exploring anaerobic isoprene metabolism remain scarce, with no isolates for study available. Here, we obtained an acetogenic isolate, designated Acetobacterium wieringae strain Y, which hydrogenated isoprene to a mixture of methyl-1-butenes at an overall rate of 288.8 ± 20.9 μM day^-1 with concomitant acetate production at a rate of 478.4 ± 5.6 μM day^-1. Physiological characterization demonstrated that isoprene was not utilized in a respiratory process; rather, isoprene promoted acetogenesis kinetically. Bioinformatic analysis and proteomics experiments revealed themore » expression of candidate ene-reductases responsible for isoprene biohydrogenation. Notably, the addition of isoprene to strain Y cultures stimulated the expression of proteins associated with the Wood-Ljungdahl pathway, indicating unresolved impacts of isoprene on carbon cycling and microbial ecology in anoxic environments (e.g., promoting CO₂ plus H₂ reductive acetogenesis while inhibiting methanogenesis). Our new findings advance understanding of microbial transformation of isoprene under anoxic conditions and suggest that anoxic environments are isoprene sinks.« less

Ultra-high performance liquid chromatography–high-resolution mass spectrometry (UPHLC–HRMS) is used to discover and monitor single or sets of biomarkers informing about metabolic processes of interest. The technique can detect 1000’s of molecules (i.e., metabolites) in a single instrument run and provide a measurement of the global metabolome, which could be a fingerprint of activity. Despite the power of this approach, technical challenges have hindered the effective use of metabolomics to interrogate microbial communities implicated in the removal of priority contaminants. Herein, our efforts to circumvent these challenges and apply this emerging systems biology technique to microbiomes relevant for contaminant biodegradation willmore » be discussed. Chlorinated ethenes impact many contaminated sites, and detoxification can be achieved by organohalide-respiring bacteria, a process currently assessed by quantitative gene-centric tools (e.g., quantitative PCR). This laboratory study monitored the metabolome of the SDC-9™ bioaugmentation consortium during cis-1,2-dichloroethene (cDCE) conversion to vinyl chloride (VC) and nontoxic ethene. Untargeted metabolomics using an UHPLC-Orbitrap mass spectrometer and performed on SDC-9™ cultures at different stages of the reductive dechlorination process detected ~10,000 spectral features per sample arising from water-soluble molecules with both known and unknown structures. Multivariate statistical techniques including partial least squares-discriminate analysis (PLSDA) identified patterns of measurable spectral features (peak patterns) that correlated with dechlorination (in)activity, and ANOVA analyses identified 18 potential biomarkers for this process. Statistical clustering of samples with these 18 features identified dechlorination activity more reliably than clustering of samples based only on chlorinated ethene concentration and Dhc 16S rRNA gene abundance data, highlighting the potential value of metabolomic workflows as an innovative site assessment and bioremediation monitoring tool.« less

The interconnection among food–energy–water (FEW) systems in meeting societal demands is broadly acknowledged. Similarly, competitive or synergistic allocations of water and energy resources for agricultural production, manufacturing, and human consumption are understood, and their economic impacts can be predicted. Far less appreciated and understood are the outcomes of the FEW nexus in response to operation changes in agricultural practices and the associated technological innovations for future generations. Also, the inter-scale and feedback effects of emerging technology-driven resource reallocation and decision-making on FEW systems are largely unknown. For example, how do the agroeconomic feedbacks of intelligent technologies influence the FEW nexusmore » of agricultural production under environmental and demographic changes? How does the necessary water allocation for powering non-powered dams and pumped-storage hydropower generation influence agricultural production and municipal water supply maintenance? How do solar and wind energy farms influence land use for agriculture and the rural economy? In turn, how can the generated solar and wind energy help reduce the cost of groundwater extraction or water desalination?« less

We report hydrology is a key factor influencing microbial degradation of emerging organic contaminants (EOCs) in soils, but the underlying mechanisms are not clear. In this study, biotic and abiotic column experiments were performed to investigate the removal and degradation of five EOCs in soils with different soil organic matter (SOM) contents under saturated and unsaturated flow conditions. In biotic experiments, 54–90% of bisphenol A (BPA) and 9–22% of ibuprofen (IBU) were removed from the aqueous phase of saturated columns due to adsorption and biodegradation. The biodegradation removed 26–65% of BPA and 1–22% of IBU. Decreasing soil pore water saturationmore » from 100 to 80% increased BPA removal to 97–100% and IBU removal to 42–43% due to increased biodegradation (67–81% for BPA and 36–39% for IBU). No significant removal of BPA and IBU was observed in SOM-removed soils under saturated and unsaturated flow conditions. The desaturation did not influence sorptive losses of BPA (< 27%) and IBU (< 7%), suggesting their negligible adsorption at air–water interfaces but increased biodegradation of BPA and IBU sorbed at SOM–water interfaces. The study shows that soil drying and SOM can synergistically degrade BPA and IBU but have no effect on recalcitrant carbamazepine, tetracycline, and ciprofloxacin.« less

Dehalococcoides mccartyi strains harboring vinyl chloride (VC) reductive dehalogenase (RDase) genes are keystone bacteria for VC detoxification in groundwater aquifers, and bioremediation monitoring regimens focus on D. mccartyi biomarkers. We isolated a novel anaerobic bacterium, “Candidatus Dehalogenimonas etheniformans” strain GP, capable of respiratory dechlorination of VC to ethene. This bacterium couples formate and hydrogen (H2) oxidation to the reduction of trichloro-ethene (TCE), all dichloroethene (DCE) isomers, and VC with acetate as the carbon source. Cultures that received formate and H2 consumed the two electron donors concomitantly at similar rates. A 16S rRNA gene-targeted quantitative PCR (qPCR) assay measured growth yieldsmore » of (1.2 ± 0.2) × 10⁸ and (1.9 ± 0.2) × 10⁸ cells per μmol of VC dechlorinated in cultures with H₂ or formate as electron donor, respectively. About 1.5-fold higher cell numbers were measured with qPCR targeting cerA, a single-copy gene encoding a putative VC RDase. A VC dechlorination rate of 215 ± 40 μmol L^-1 day^-1 was measured at 30°C, with about 25% of this activity occurring at 15°C. Increasing NaCl concentrations progressively impacted VC dechlorination rates, and dechlorination ceased at 15 g NaCl L^-1. During growth with TCE, all DCE isomers were intermediates. Tetrachloroethene was not dechlorinated and inhibited dechlorination of other chlorinated ethenes. Carbon monoxide formed and accumulated as a metabolic by-product in dechlorinating cultures and impacted reductive dechlorination activity. Finally, the isolation of a new Dehalogenimonas species able to effectively dechlorinate toxic chlorinated ethenes to benign ethene expands our understanding of the reductive dechlorination process, with implications for bioremediation and environmental monitoring.« less