Dehalogenation of Chlorinated Ethenes to Ethene by a Novel Isolate, “Candidatus Dehalogenimonas etheniformans”

Chen, Gao; Kara Murdoch, Fadime; Xie, Yongchao; Murdoch, Robert W.; Cui, Yiru; Yang, Yi; Yan, Jun; Key, Trent A.; Löffler, Frank E.

doi:10.1128/aem.00443-22

Title: Dehalogenation of Chlorinated Ethenes to Ethene by a Novel Isolate, “Candidatus Dehalogenimonas etheniformans”

Journal Article · Tue Jun 28 00:00:00 UTC 2022 · Applied and Environmental Microbiology

DOI: https://doi.org/10.1128/aem.00443-22 · OSTI ID:1871866

^[1]; Kara Murdoch, Fadime ^[2]; Xie, Yongchao ^[3]; Murdoch, Robert W. ^[2]; Cui, Yiru ^[4];

^[5];

^[6]; Key, Trent A. ^[7];

^[8]

Univ. of Tennessee, Knoxville, TN (United States). Center for Environmental Biotechnology; Univ. of Tennessee, Knoxville, TN (United States)
Univ. of Tennessee, Knoxville, TN (United States). Center for Environmental Biotechnology
Univ. of Tennessee, Knoxville, TN (United States). Center for Environmental Biotechnology; Univ. of Tennessee, Knoxville, TN (United States); Univ. of California, Los Angeles, CA (United States)
Chinese Academy of Sciences, Shenyang (China). Inst. of Applied Ecology, Key Laboratory of Pollution Ecology and Environmental Engineering; University of Chinese Academy of Sciences, Beijing (China)
Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, Liaoning, China
Chinese Academy of Sciences, Shenyang (China). Inst. of Applied Ecology, Key Laboratory of Pollution Ecology and Environmental Engineering
ExxonMobil Environmental and Property Solutions Company, Spring, TX (United States)
Univ. of Tennessee, Knoxville, TN (United States). Center for Environmental Biotechnology; Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Dehalococcoides mccartyi strains harboring vinyl chloride (VC) reductive dehalogenase (RDase) genes are keystone bacteria for VC detoxification in groundwater aquifers, and bioremediation monitoring regimens focus on D. mccartyi biomarkers. We isolated a novel anaerobic bacterium, “Candidatus Dehalogenimonas etheniformans” strain GP, capable of respiratory dechlorination of VC to ethene. This bacterium couples formate and hydrogen (H2) oxidation to the reduction of trichloro-ethene (TCE), all dichloroethene (DCE) isomers, and VC with acetate as the carbon source. Cultures that received formate and H2 consumed the two electron donors concomitantly at similar rates. A 16S rRNA gene-targeted quantitative PCR (qPCR) assay measured growth yields of (1.2 ± 0.2) × 10⁸ and (1.9 ± 0.2) × 10⁸ cells per μmol of VC dechlorinated in cultures with H₂ or formate as electron donor, respectively. About 1.5-fold higher cell numbers were measured with qPCR targeting cerA, a single-copy gene encoding a putative VC RDase. A VC dechlorination rate of 215 ± 40 μmol L^-1 day^-1 was measured at 30°C, with about 25% of this activity occurring at 15°C. Increasing NaCl concentrations progressively impacted VC dechlorination rates, and dechlorination ceased at 15 g NaCl L^-1. During growth with TCE, all DCE isomers were intermediates. Tetrachloroethene was not dechlorinated and inhibited dechlorination of other chlorinated ethenes. Carbon monoxide formed and accumulated as a metabolic by-product in dechlorinating cultures and impacted reductive dechlorination activity. Finally, the isolation of a new Dehalogenimonas species able to effectively dechlorinate toxic chlorinated ethenes to benign ethene expands our understanding of the reductive dechlorination process, with implications for bioremediation and environmental monitoring.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1871866

Journal Information:: Applied and Environmental Microbiology, Journal Name: Applied and Environmental Microbiology Journal Issue: 12 Vol. 88; ISSN 0099-2240

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

References (63)

Dehalococcoides and Reductive Dechlorination of Chlorinated Solvents Löffler, Frank E.; Ritalahti, Kirsti M.; Zinder, Stephen H. Bioaugmentation for Groundwater Remediation https://doi.org/10.1007/978-1-4614-4115-1_2	book	July 2012
The Genus Dehalococcoides Zinder, Stephen H. Organohalide-Respiring Bacteria https://doi.org/10.1007/978-3-662-49875-0_6	book	January 2016
Characterization of microbial community structure and population dynamics of tetrachloroethene-dechlorinating tidal mudflat communities Lee, Jaejin; Lee, Tae Kwon; Löffler, Frank E. Biodegradation, Vol. 22, Issue 4 https://doi.org/10.1007/s10532-010-9429-x	journal	October 2010
Growth of Dehalococcoides mccartyi species in an autotrophic consortium producing limited acetate Ding, Chang; Alvarez-Cohen, Lisa; He, Jianzhong Biodegradation, Vol. 29, Issue 5 https://doi.org/10.1007/s10532-018-9846-9	journal	August 2018
Enrichment, Cultivation, and Detection of Reductively Dechlorinating Bacteria Löffler, Frank E.; Sanford, Robert A.; Ritalahti, Kirsti M. Methods in Enzymology https://doi.org/10.1016/S0076-6879(05)97005-5	book	January 2005
Harnessing microbial activities for environmental cleanup Löffler, Frank E.; Edwards, Elizabeth A. Current Opinion in Biotechnology, Vol. 17, Issue 3 https://doi.org/10.1016/j.copbio.2006.05.001	journal	June 2006
Carbon monoxide is a poison … to microbes! CO as a bactericidal molecule Chin, Beek Yoke; Otterbein, Leo E. Current Opinion in Pharmacology, Vol. 9, Issue 4 https://doi.org/10.1016/j.coph.2009.06.025	journal	August 2009
Quantitative real-time PCR (qPCR) detection chemistries affect enumeration of the Dehalococcoides 16S rRNA gene in groundwater Hatt, Janet K.; Löffler, Frank E. Journal of Microbiological Methods, Vol. 88, Issue 2 https://doi.org/10.1016/j.mimet.2011.12.005	journal	February 2012
Reductive dechlorination of tetrachloroethene in marine sediments: Biodiversity and dehalorespiring capabilities of the indigenous microbes Matturro, B.; Presta, E.; Rossetti, S. Science of The Total Environment, Vol. 545-546 https://doi.org/10.1016/j.scitotenv.2015.12.098	journal	March 2016
‘ Candidatus Dichloromethanomonas elyunquensis’ gen. nov., sp. nov., a dichloromethane-degrading anaerobe of the Peptococcaceae family Kleindienst, Sara; Higgins, Steven A.; Tsementzi, Despina Systematic and Applied Microbiology, Vol. 40, Issue 3 https://doi.org/10.1016/j.syapm.2016.12.001	journal	April 2017
Temperature dependence of anaerobic TCE-dechlorination in a highly enriched Dehalococcoides-containing culture Friis, Anne K.; Heimann, Axel C.; Jakobsen, Rasmus Water Research, Vol. 41, Issue 2 https://doi.org/10.1016/j.watres.2006.09.026	journal	January 2007
Impacts of low-temperature thermal treatment on microbial detoxification of tetrachloroethene under continuous flow conditions Marcet, Tyler F.; Cápiro, Natalie L.; Yang, Yi Water Research, Vol. 145 https://doi.org/10.1016/j.watres.2018.07.076	journal	November 2018
Respiratory Vinyl Chloride Reductive Dechlorination to Ethene in TceA-Expressing Dehalococcoides mccartyi Yan, Jun; Wang, Jingjing; Villalobos Solis, Manuel I. Environmental Science & Technology, Vol. 55, Issue 8 https://doi.org/10.1021/acs.est.0c07354	journal	March 2021
A Data Mining Approach to Predict In Situ Detoxification Potential of Chlorinated Ethenes Lee, Jaejin; Im, Jeongdae; Kim, Ungtae Environmental Science & Technology, Vol. 50, Issue 10 https://doi.org/10.1021/acs.est.5b05090	journal	May 2016
Organohalide Respiration with Chlorinated Ethenes under Low pH Conditions Yang, Yi; Cápiro, Natalie L.; Marcet, Tyler F. Environmental Science & Technology, Vol. 51, Issue 15 https://doi.org/10.1021/acs.est.7b01510	journal	July 2017
Normalized Quantitative PCR Measurements as Predictors for Ethene Formation at Sites Impacted with Chlorinated Ethenes Clark, Katherine; Taggart, Dora M.; Baldwin, Brett R. Environmental Science & Technology, Vol. 52, Issue 22 https://doi.org/10.1021/acs.est.8b04373	journal	October 2018
Nitrous Oxide Is a Potent Inhibitor of Bacterial Reductive Dechlorination Yin, Yongchao; Yan, Jun; Chen, Gao Environmental Science & Technology, Vol. 53, Issue 2 https://doi.org/10.1021/acs.est.8b05871	journal	December 2018
Genome Sequence, Proteome Profile, and Identification of a Multiprotein Reductive Dehalogenase Complex in Dehalogenimonas alkenigignens Strain BRE15M Trueba-Santiso, Alba; Wasmund, Kenneth; Soder-Walz, Jesica M. Journal of Proteome Research, Vol. 20, Issue 1 https://doi.org/10.1021/acs.jproteome.0c00569	journal	September 2020
Natural Formation of Vinyl Chloride in the Terrestrial Environment Keppler, Frank; Borchers, Reinhard; Pracht, Jens Environmental Science & Technology, Vol. 36, Issue 11 https://doi.org/10.1021/es015611l	journal	June 2002
Vinyl Chloride and cis -Dichloroethene Dechlorination Kinetics and Microorganism Growth under Substrate Limiting Conditions Cupples, Alison M.; Spormann, Alfred M.; McCarty, Perry L. Environmental Science & Technology, Vol. 38, Issue 4 https://doi.org/10.1021/es0348647	journal	February 2004
Anaerobic Microbial Reductive Dechlorination of Tetrachloroethene to Predominately trans -1,2-Dichloroethene Griffin, Benjamin M.; Tiedje, James M.; Löffler, Frank E. Environmental Science & Technology, Vol. 38, Issue 16 https://doi.org/10.1021/es035439g	journal	August 2004
1,1-Dichloroethene as a Predominant Intermediate of Microbial Trichloroethene Reduction Zhang, Jingjing; Joslyn, Andrew P.; Chiu, Pei C. Environmental Science & Technology, Vol. 40, Issue 6 https://doi.org/10.1021/es051829m	journal	February 2006
Chlorinated Solvents in Groundwater of the United States Moran, Michael J.; Zogorski, John S.; Squillace, Paul J. Environmental Science & Technology, Vol. 41, Issue 1 https://doi.org/10.1021/es061553y	journal	January 2007
Design and Application of an Internal Amplification Control to Improve Dehalococcoides mccartyi 16S rRNA Gene Enumeration by qPCR Hatt, Janet K.; Ritalahti, Kirsti M.; Ogles, Dora M. Environmental Science & Technology, Vol. 47, Issue 19 https://doi.org/10.1021/es4019817	journal	September 2013
Sustainable syntrophic growth of Dehalococcoides ethenogenes strain 195 with Desulfovibrio vulgaris Hildenborough and Methanobacterium congolense: global transcriptomic and proteomic analyses Men, Yujie; Feil, Helene; VerBerkmoes, Nathan C. The ISME Journal, Vol. 6, Issue 2 https://doi.org/10.1038/ismej.2011.111	journal	September 2011
Grape pomace compost harbors organohalide-respiring Dehalogenimonas species with novel reductive dehalogenase genes Yang, Yi; Higgins, Steven A.; Yan, Jun The ISME Journal, Vol. 11, Issue 12 https://doi.org/10.1038/ismej.2017.127	journal	August 2017
Detoxification of vinyl chloride to ethene coupled to growth of an anaerobic bacterium He, Jianzhong; Ritalahti, Kirsti M.; Yang, Kun-Lin Nature, Vol. 424, Issue 6944 https://doi.org/10.1038/nature01717	journal	July 2003
Gene transfer agents: phage-like elements of genetic exchange Lang, Andrew S.; Zhaxybayeva, Olga; Beatty, J. Thomas Nature Reviews Microbiology, Vol. 10, Issue 7 https://doi.org/10.1038/nrmicro2802	journal	June 2012
Mineralization versus fermentation: evidence for two distinct anaerobic bacterial degradation pathways for dichloromethane Chen, Gao; Fisch, Alexander R.; Gibson, Caleb M. The ISME Journal, Vol. 14, Issue 4 https://doi.org/10.1038/s41396-019-0579-5	journal	January 2020
A recent survey of naturally occurring organohalogen compounds Gribble, Gordon W. Environmental Chemistry, Vol. 12, Issue 4 https://doi.org/10.1071/EN15002	journal	January 2015
Incomplete Wood-Ljungdahl pathway facilitates one-carbon metabolism in organohalide-respiring Dehalococcoides mccartyi Zhuang, W. -Q.; Yi, S.; Bill, M. Proceedings of the National Academy of Sciences, Vol. 111, Issue 17 https://doi.org/10.1073/pnas.1321542111	journal	April 2014
SINA: Accurate high-throughput multiple sequence alignment of ribosomal RNA genes Pruesse, Elmar; Peplies, Jörg; Glöckner, Frank Oliver Bioinformatics, Vol. 28, Issue 14 https://doi.org/10.1093/bioinformatics/bts252	journal	May 2012
RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies Stamatakis, Alexandros Bioinformatics, Vol. 30, Issue 9 https://doi.org/10.1093/bioinformatics/btu033	journal	January 2014
Resilience and recovery of Dehalococcoides mccartyi following low pH exposure Yang, Yi; Cápiro, Natalie L.; Yan, Jun FEMS Microbiology Ecology, Vol. 93, Issue 12 https://doi.org/10.1093/femsec/fix130	journal	October 2017
Interactive Tree Of Life (iTOL) v5: an online tool for phylogenetic tree display and annotation Letunic, Ivica; Bork, Peer Nucleic Acids Research, Vol. 49, Issue W1 https://doi.org/10.1093/nar/gkab301	journal	April 2021
The SILVA ribosomal RNA gene database project: improved data processing and web-based tools Quast, Christian; Pruesse, Elmar; Yilmaz, Pelin Nucleic Acids Research, Vol. 41, Issue D1 https://doi.org/10.1093/nar/gks1219	journal	November 2012
Dehalogenimonas lykanthroporepellens gen. nov., sp. nov., a reductively dehalogenating bacterium isolated from chlorinated solvent-contaminated groundwater Moe, W. M.; Yan, J.; Nobre, M. F. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 59, Issue 11 https://doi.org/10.1099/ijs.0.011502-0	journal	July 2009
Dehalococcoides mccartyi gen. nov., sp. nov., obligately organohalide-respiring anaerobic bacteria relevant to halogen cycling and bioremediation, belong to a novel bacterial class, Dehalococcoidia classis nov., order Dehalococcoidales ord. nov. and family Dehalococcoidaceae fam. nov., within the phylum Chloroflexi Loffler, F. E.; Yan, J.; Ritalahti, K. M. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 63, Issue Pt 2 https://doi.org/10.1099/ijs.0.034926-0	journal	April 2012
Dehalogenimonas alkenigignens sp. nov., a chlorinated-alkane-dehalogenating bacterium isolated from groundwater Bowman, Kimberly S.; Nobre, M. Fernanda; da Costa, Milton S. International Journal of Systematic and Evolutionary Microbiology, Vol. 63, Issue Pt_4 https://doi.org/10.1099/ijs.0.045054-0	journal	April 2013
Dehalogenimonas formicexedens sp. nov., a chlorinated alkane-respiring bacterium isolated from contaminated groundwater Key, Trent A.; Bowman, Kimberly S.; Lee, Imchang International Journal of Systematic and Evolutionary Microbiology, Vol. 67, Issue 5, p. 1366-1373 https://doi.org/10.1099/ijsem.0.001819	journal	May 2017
Mutualistic interaction between dichloromethane- and chloromethane-degrading bacteria in an anaerobic mixed culture: Mutualistic degradation of chlorinated methanes Chen, Gao; Kleindienst, Sara; Griffiths, Daniel R. Environmental Microbiology, Vol. 19, Issue 11 https://doi.org/10.1111/1462-2920.13945	journal	October 2017
Isolation and characterization of Dehalococcoides sp. strain FL2, a trichloroethene (TCE)- and 1,2-dichloroethene-respiring anaerobe He, Jianzhong; Sung, Youlboong; Krajmalnik-Brown, Rosa Environmental Microbiology, Vol. 7, Issue 9 https://doi.org/10.1111/j.1462-2920.2005.00830.x	journal	September 2005
Isolation of novel bacteria within the Chloroflexi capable of reductive dechlorination of 1,2,3-trichloropropane Yan, J.; Rash, B. A.; Rainey, F. A. Environmental Microbiology, Vol. 11, Issue 4 https://doi.org/10.1111/j.1462-2920.2008.01804.x	journal	April 2009
Isolation of a Bacterium That Reductively Dechlorinates Tetrachloroethene to Ethene Maymo-Gatell, X. Science, Vol. 276, Issue 5318 https://doi.org/10.1126/science.276.5318.1568	journal	June 1997
Discovery of a trans -Dichloroethene-Respiring Dehalogenimonas Species in the 1,1,2,2-Tetrachloroethane-Dechlorinating WBC-2 Consortium Manchester, Marie J.; Hug, Laura A.; Zarek, Matt Applied and Environmental Microbiology, Vol. 78, Issue 15 https://doi.org/10.1128/AEM.00384-12	journal	August 2012
Dehalorespiration with Polychlorinated Biphenyls by an Anaerobic Ultramicrobacterium May, Harold D.; Miller, Greg S.; Kjellerup, Birthe V. Applied and Environmental Microbiology, Vol. 74, Issue 7 https://doi.org/10.1128/AEM.01450-07	journal	April 2008
Functional Characterization of Reductive Dehalogenases by Using Blue Native Polyacrylamide Gel Electrophoresis Tang, Shuiquan; Chan, Winnie W. M.; Fletcher, Kelly E. Applied and Environmental Microbiology, Vol. 79, Issue 3 https://doi.org/10.1128/AEM.01873-12	journal	November 2012
Ultrastructure of Organohalide-Respiring Dehalococcoidia Revealed by Cryo-Electron Tomography Sexton, Danielle L.; Chen, Gao; Kara Murdoch, Fadime Applied and Environmental Microbiology, Vol. 88, Issue 2 https://doi.org/10.1128/AEM.01906-21	journal	January 2022
Dehalogenimonas sp. Strain WBC-2 Genome and Identification of Its trans -Dichloroethene Reductive Dehalogenase, TdrA Molenda, Olivia; Quaile, Andrew T.; Edwards, Elizabeth A. Applied and Environmental Microbiology, Vol. 82, Issue 1 https://doi.org/10.1128/AEM.02017-15	journal	October 2015
Reductive Dechlorination of Chlorinated Ethenes and 1,2-Dichloroethane by “Dehalococcoides ethenogenes” 195 Maymó-Gatell, Xavier; Anguish, Timothy; Zinder, Stephen H. Applied and Environmental Microbiology, Vol. 65, Issue 7 https://doi.org/10.1128/AEM.65.7.3108-3113.1999	journal	July 1999
Growth of a Dehalococcoides-Like Microorganism on Vinyl Chloride and cis-Dichloroethene as Electron Acceptors as Determined by Competitive PCR Cupples, A. M.; Spormann, A. M.; McCarty, P. L. Applied and Environmental Microbiology, Vol. 69, Issue 2 https://doi.org/10.1128/AEM.69.2.953-959.2003	journal	February 2003
Genetic Identification of a Putative Vinyl Chloride Reductase in Dehalococcoides sp. Strain BAV1 Krajmalnik-Brown, R.; Holscher, T.; Thomson, I. N. Applied and Environmental Microbiology, Vol. 70, Issue 10 https://doi.org/10.1128/AEM.70.10.6347-6351.2004	journal	October 2004
Molecular Identification of the Catabolic Vinyl Chloride Reductase from Dehalococcoides sp. Strain VS and Its Environmental Distribution Muller, J. A.; Rosner, B. M.; von Abendroth, G. Applied and Environmental Microbiology, Vol. 70, Issue 8 https://doi.org/10.1128/AEM.70.8.4880-4888.2004	journal	August 2004
Chloroethene Biodegradation in Sediments at 4°C Bradley, P. M.; Richmond, S.; Chapelle, F. H. Applied and Environmental Microbiology, Vol. 71, Issue 10 https://doi.org/10.1128/AEM.71.10.6414-6417.2005	journal	October 2005
Quantitative PCR Confirms Purity of Strain GT, a Novel Trichloroethene-to-Ethene-Respiring Dehalococcoides Isolate Sung, Y.; Ritalahti, K. M.; Apkarian, R. P. Applied and Environmental Microbiology, Vol. 72, Issue 3 https://doi.org/10.1128/AEM.72.3.1980-1987.2006	journal	March 2006
Quantitative PCR Targeting 16S rRNA and Reductive Dehalogenase Genes Simultaneously Monitors Multiple Dehalococcoides Strains Ritalahti, K. M.; Amos, B. K.; Sung, Y. Applied and Environmental Microbiology, Vol. 72, Issue 4 https://doi.org/10.1128/AEM.72.4.2765-2774.2006	journal	April 2006
Genome Sequence of “ Candidatus Dehalogenimonas etheniformans” Strain GP, a Vinyl Chloride-Respiring Anaerobe Yang, Yi; Yan, Jun; Li, Xiuying Microbiology Resource Announcements, Vol. 9, Issue 50 https://doi.org/10.1128/MRA.01212-20	journal	December 2020
Anaerobic Microbial Metabolism of Dichloroacetate Chen, Gao; Jiang, Nannan; Villalobos Solis, Manuel I. mBio, Vol. 12, Issue 2 https://doi.org/10.1128/mBio.00537-21	journal	April 2021
Anaerobic Microbial Dehalogenation Smidt, Hauke; de Vos, Willem M. Annual Review of Microbiology, Vol. 58, Issue 1 https://doi.org/10.1146/annurev.micro.58.030603.123600	journal	October 2004
Dehalogenimonas spp. can Reductively Dehalogenate High Concentrations of 1,2-Dichloroethane, 1,2-Dichloropropane, and 1,1,2-Trichloroethane Maness, Andrew D.; Bowman, Kimberly S.; Yan, Jun AMB Express, Vol. 2, Issue 1 https://doi.org/10.1186/2191-0855-2-54	journal	January 2012
Genome sequence of the organohalide-respiring Dehalogenimonas alkenigignens type strain (IP3-3T) Key, Trent A.; Richmond, Dray P.; Bowman, Kimberly S. Standards in Genomic Sciences, Vol. 11, Issue 1 https://doi.org/10.1186/s40793-016-0165-7	journal	June 2016
Ecogenomics of microbial communities in bioremediation of chlorinated contaminated sites Maphosa, Farai; Lieten, Shakti H.; Dinkla, Inez Frontiers in Microbiology, Vol. 3 https://doi.org/10.3389/fmicb.2012.00351	journal	January 2012
Compilation of Henry's law constants (version 4.0) for water as solvent Sander, R. Atmospheric Chemistry and Physics, Vol. 15, Issue 8 https://doi.org/10.5194/acp-15-4399-2015	journal	January 2015

Similar Records

Respiratory Vinyl Chloride Reductive Dechlorination to Ethene in TceA-Expressing Dehalococcoides mccartyi

Journal Article · Mon Mar 08 00:00:00 UTC 2021 · Environmental Science and Technology · OSTI ID:1830109

Yan, Jun; Wang, Jingjing; Villalobos Solis, Manuel I.; +7 more

The corrinoid cofactor of reductive dehalogenases affects dechlorination rates and extents in organohalide-respiring Dehalococcoides mccartyi

Journal Article · Tue Nov 10 00:00:00 UTC 2015 · The ISME Journal · OSTI ID:1468287

Yan, Jun; Şimşir, Burcu; Farmer, Abigail T.; +4 more

Grape pomace compost harbors organohalide-respiring Dehalogenimonas species with novel reductive dehalogenase genes

Journal Article · Tue Aug 15 00:00:00 UTC 2017 · The ISME Journal · OSTI ID:1408046

Yang, Yi; Higgins, Steven A.; Yan, Jun; +7 more

Related Subjects

59 BASIC BIOLOGICAL SCIENCES

Title: Dehalogenation of Chlorinated Ethenes to Ethene by a Novel Isolate, “Candidatus Dehalogenimonas etheniformans”

Citation Formats

References (63)

Similar Records

Related Subjects