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Title: Soluble methane monooxygenase gene clusters from trichloroethylene-degrading Methylomonas sp. strains and detection of methanotrophs during in situ bioremediation

Abstract

The soluble MMO (sMMO) gene clusters from group 1 methanotrophs were characterized. An 9.1-kb KpmI fragment from Methylomonas sp. strain KSWIII and a 7.5-kb SalI fragment from methylomonas sp. strain KSPIII which contained the sMMO gene clusters were cloned and sequenced. The sequences of these two fragments were almost identical. The sMMO gene clusters in the fragment consisted of six open reading frames which were 52 to 79% similar to the corresponding genes of previously described sMMO gene clusters of the group 2 and group 10 methanotrophs. The phylogenetic analysis of the predicted amino acid sequences of sMMO demonstrated that the sMMOs from these strains were closer to that from M. capsulatus Bath in the group X methanotrophs than to those from Methylosinus trichosporium OB3b and Methylocystis sp. strain M in the group 2 methanotrophs. Based on the sequence data of sMMO genes of the strains and other methanotrophs, the authors designed a new PCR primer to amplify sMMO gene fragments of all the known methanotrophs harboring the mmoX gene. The primer set was successfully used for detecting methanotrophs in the ground-water of trichloroethylene-contaminated sites during in situ-biostimulation treatments.

Authors:
; ; ; ; ; ;
Publication Date:
Research Org.:
National Inst. of Bioscience and Human-Technology, Ibaraki (JP)
OSTI Identifier:
20006173
Resource Type:
Journal Article
Journal Name:
Applied and Environmental Microbiology
Additional Journal Information:
Journal Volume: 65; Journal Issue: 12; Other Information: PBD: Dec 1999; Journal ID: ISSN 0099-2240
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; REMEDIAL ACTION; BIODEGRADATION; CHLORINATED ALIPHATIC HYDROCARBONS; SOILS; GROUND WATER; IN-SITU PROCESSING; GENES; OXYGENASES; METHANOTROPHIC BACTERIA

Citation Formats

Shigematsu, Toru, Hanada, Satoshi, Eguchi, Masahiro, Kamagata, Yoichi, Kanagawa, Takahiro, Kurane,, and Ryuichiro,. Soluble methane monooxygenase gene clusters from trichloroethylene-degrading Methylomonas sp. strains and detection of methanotrophs during in situ bioremediation. United States: N. p., 1999. Web.
Shigematsu, Toru, Hanada, Satoshi, Eguchi, Masahiro, Kamagata, Yoichi, Kanagawa, Takahiro, Kurane,, & Ryuichiro,. Soluble methane monooxygenase gene clusters from trichloroethylene-degrading Methylomonas sp. strains and detection of methanotrophs during in situ bioremediation. United States.
Shigematsu, Toru, Hanada, Satoshi, Eguchi, Masahiro, Kamagata, Yoichi, Kanagawa, Takahiro, Kurane,, and Ryuichiro,. Wed . "Soluble methane monooxygenase gene clusters from trichloroethylene-degrading Methylomonas sp. strains and detection of methanotrophs during in situ bioremediation". United States.
@article{osti_20006173,
title = {Soluble methane monooxygenase gene clusters from trichloroethylene-degrading Methylomonas sp. strains and detection of methanotrophs during in situ bioremediation},
author = {Shigematsu, Toru and Hanada, Satoshi and Eguchi, Masahiro and Kamagata, Yoichi and Kanagawa, Takahiro and Kurane, and Ryuichiro,},
abstractNote = {The soluble MMO (sMMO) gene clusters from group 1 methanotrophs were characterized. An 9.1-kb KpmI fragment from Methylomonas sp. strain KSWIII and a 7.5-kb SalI fragment from methylomonas sp. strain KSPIII which contained the sMMO gene clusters were cloned and sequenced. The sequences of these two fragments were almost identical. The sMMO gene clusters in the fragment consisted of six open reading frames which were 52 to 79% similar to the corresponding genes of previously described sMMO gene clusters of the group 2 and group 10 methanotrophs. The phylogenetic analysis of the predicted amino acid sequences of sMMO demonstrated that the sMMOs from these strains were closer to that from M. capsulatus Bath in the group X methanotrophs than to those from Methylosinus trichosporium OB3b and Methylocystis sp. strain M in the group 2 methanotrophs. Based on the sequence data of sMMO genes of the strains and other methanotrophs, the authors designed a new PCR primer to amplify sMMO gene fragments of all the known methanotrophs harboring the mmoX gene. The primer set was successfully used for detecting methanotrophs in the ground-water of trichloroethylene-contaminated sites during in situ-biostimulation treatments.},
doi = {},
url = {https://www.osti.gov/biblio/20006173}, journal = {Applied and Environmental Microbiology},
issn = {0099-2240},
number = 12,
volume = 65,
place = {United States},
year = {1999},
month = {12}
}