Soluble methane monooxygenase gene clusters from trichloroethylene-degrading Methylomonas sp. strains and detection of methanotrophs during in situ bioremediation
The soluble MMO (sMMO) gene clusters from group 1 methanotrophs were characterized. An 9.1-kb KpmI fragment from Methylomonas sp. strain KSWIII and a 7.5-kb SalI fragment from methylomonas sp. strain KSPIII which contained the sMMO gene clusters were cloned and sequenced. The sequences of these two fragments were almost identical. The sMMO gene clusters in the fragment consisted of six open reading frames which were 52 to 79% similar to the corresponding genes of previously described sMMO gene clusters of the group 2 and group 10 methanotrophs. The phylogenetic analysis of the predicted amino acid sequences of sMMO demonstrated that the sMMOs from these strains were closer to that from M. capsulatus Bath in the group X methanotrophs than to those from Methylosinus trichosporium OB3b and Methylocystis sp. strain M in the group 2 methanotrophs. Based on the sequence data of sMMO genes of the strains and other methanotrophs, the authors designed a new PCR primer to amplify sMMO gene fragments of all the known methanotrophs harboring the mmoX gene. The primer set was successfully used for detecting methanotrophs in the ground-water of trichloroethylene-contaminated sites during in situ-biostimulation treatments.
- Research Organization:
- National Inst. of Bioscience and Human-Technology, Ibaraki (JP)
- OSTI ID:
- 20006173
- Journal Information:
- Applied and Environmental Microbiology, Vol. 65, Issue 12; Other Information: PBD: Dec 1999; ISSN 0099-2240
- Country of Publication:
- United States
- Language:
- English
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