Soluble methane monooxygenase production and trichloroethylene degradation by a type I methanotroph, Methylomonas methanica 68-1

Journal Article · Thu Apr 01 04:00:00 EST 1993 · Applied and Environmental Microbiology; (United States)

OSTI ID:6466387

Koh, Sung-Cheol; Bowman, J P; Sayler, G S ^[1]

---

1. Univ. of Tennessee, Knoxville (United States)

Soluble methane monooxygenase (sMMO), present in certain methanotrophic bacteria, can oxidize a wide range of carbon substrates including halogenated aliphatic compounds such as trichloroethylene (TCE), a significant ground water pollutant. This study reports the existence of sMMO activity in a type I methanotroph (Methylosinus trichosporium 68-1) isolated from a TCE-contaminated aquifer. The sMMO of 68-1 was compared with that of Methylosinus trichosporium OB3b in terms of TCE degradation kinetics and genetic homology. This study shows that a typical type I methanotrophy (68-1) has the ability to produce sMMO and rapidly degrade TCE, producing a relatively high biomass under the apparent nonoptimal state of copper limitation. As a result, this strain may have considerable bioremediation potential. Further studies will be necessary to determine exactly how the 68-1 sMMO differs from the sMMOs of OB3b and Methylococcus capsulatus both in terms of DNA and protein sequences. 48 refs., 4 figs., 2 tabs.

• DOE Contract Number: AC05-84OR21400
• OSTI ID: 6466387
• Journal Information: Applied and Environmental Microbiology; (United States), Journal Name: Applied and Environmental Microbiology; (United States) Vol. 59:4; ISSN AEMIDF; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AQUIFERS
BACTERIA
BIODEGRADATION
BIOMASS
CARBON
CHEMICAL REACTIONS
CLEANING
CONTAMINATION
COPPER
DECOMPOSITION
DECONTAMINATION
DNA
ELEMENTS
ENERGY SOURCES
ENZYMES
GROUND WATER
HALOGENATED ALIPHATIC HYDROCARBONS
HYDROGEN COMPOUNDS
KINETICS
METALS
METHANOTROPHIC BACTERIA
MICROORGANISMS
NONMETALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
OXYGEN COMPOUNDS
POLLUTANTS
PROTEINS
RENEWABLE ENERGY SOURCES
SUBSTRATES
TRANSITION ELEMENTS
WATER