Soluble methane monooxygenase production and trichloroethylene degradation by a type I methanotroph, Methylomonas methanica 68-1
- Univ. of Tennessee, Knoxville (United States)
Soluble methane monooxygenase (sMMO), present in certain methanotrophic bacteria, can oxidize a wide range of carbon substrates including halogenated aliphatic compounds such as trichloroethylene (TCE), a significant ground water pollutant. This study reports the existence of sMMO activity in a type I methanotroph (Methylosinus trichosporium 68-1) isolated from a TCE-contaminated aquifer. The sMMO of 68-1 was compared with that of Methylosinus trichosporium OB3b in terms of TCE degradation kinetics and genetic homology. This study shows that a typical type I methanotrophy (68-1) has the ability to produce sMMO and rapidly degrade TCE, producing a relatively high biomass under the apparent nonoptimal state of copper limitation. As a result, this strain may have considerable bioremediation potential. Further studies will be necessary to determine exactly how the 68-1 sMMO differs from the sMMOs of OB3b and Methylococcus capsulatus both in terms of DNA and protein sequences. 48 refs., 4 figs., 2 tabs.
- DOE Contract Number:
- AC05-84OR21400
- OSTI ID:
- 6466387
- Journal Information:
- Applied and Environmental Microbiology; (United States), Vol. 59:4; ISSN 0099-2240
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GROUND WATER
CONTAMINATION
HALOGENATED ALIPHATIC HYDROCARBONS
BIODEGRADATION
METHANOTROPHIC BACTERIA
DECONTAMINATION
AQUIFERS
BIOMASS
CARBON
COPPER
DNA
ENZYMES
KINETICS
POLLUTANTS
SUBSTRATES
BACTERIA
CHEMICAL REACTIONS
CLEANING
DECOMPOSITION
ELEMENTS
ENERGY SOURCES
HYDROGEN COMPOUNDS
METALS
MICROORGANISMS
NONMETALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
OXYGEN COMPOUNDS
PROTEINS
RENEWABLE ENERGY SOURCES
TRANSITION ELEMENTS
WATER
560300* - Chemicals Metabolism & Toxicology