Airy light-sheet Raman imaging
Abstract
Light-sheet fluorescence microscopy has greatly improved the speed and overall photostability of optically sectioning cellular and multi-cellular specimens. Similar gains have also been conferred by light-sheet Raman imaging; these schemes, however, rely on diffraction limited Gaussian beams that hinder the uniformity and size of the imaging field-of-view, and, as such, the resulting throughput rates. Here, we demonstrate that a digitally scanned Airy beam increases the Raman imaging throughput rates by more than an order of magnitude than conventional diffraction-limited beams. Overall, this, spectrometer-less, approach enabled 3D imaging of microparticles with high contrast and 1 µm axial resolution at 300 msec integration times per plane and orders of magnitude lower irradiation density than coherent Raman imaging schemes. We detail the apparatus and its performance, as well as its compatibility with fluorescence light-sheet and quantitative-phase imaging towards rapid and low phototoxicity multimodal imaging.
- Authors:
- Publication Date:
- Research Org.:
- Univ. of Idaho, Moscow, ID (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1821772
- Alternate Identifier(s):
- OSTI ID: 1818773
- Grant/Contract Number:
- SC0019249
- Resource Type:
- Published Article
- Journal Name:
- Optics Express
- Additional Journal Information:
- Journal Name: Optics Express Journal Volume: 29 Journal Issue: 20; Journal ID: ISSN 1094-4087
- Publisher:
- Optical Society of America
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 47 OTHER INSTRUMENTATION; bioimaging; Raman imaging; light-sheet microscopy
Citation Formats
Subedi, N. R., Yaraghi, S., Jung, P. S., Kukal, G., McDonald, A. G., Christodoulides, D. N., and Vasdekis, A. E. Airy light-sheet Raman imaging. United States: N. p., 2021.
Web. doi:10.1364/OE.435293.
Subedi, N. R., Yaraghi, S., Jung, P. S., Kukal, G., McDonald, A. G., Christodoulides, D. N., & Vasdekis, A. E. Airy light-sheet Raman imaging. United States. https://doi.org/10.1364/OE.435293
Subedi, N. R., Yaraghi, S., Jung, P. S., Kukal, G., McDonald, A. G., Christodoulides, D. N., and Vasdekis, A. E. Tue .
"Airy light-sheet Raman imaging". United States. https://doi.org/10.1364/OE.435293.
@article{osti_1821772,
title = {Airy light-sheet Raman imaging},
author = {Subedi, N. R. and Yaraghi, S. and Jung, P. S. and Kukal, G. and McDonald, A. G. and Christodoulides, D. N. and Vasdekis, A. E.},
abstractNote = {Light-sheet fluorescence microscopy has greatly improved the speed and overall photostability of optically sectioning cellular and multi-cellular specimens. Similar gains have also been conferred by light-sheet Raman imaging; these schemes, however, rely on diffraction limited Gaussian beams that hinder the uniformity and size of the imaging field-of-view, and, as such, the resulting throughput rates. Here, we demonstrate that a digitally scanned Airy beam increases the Raman imaging throughput rates by more than an order of magnitude than conventional diffraction-limited beams. Overall, this, spectrometer-less, approach enabled 3D imaging of microparticles with high contrast and 1 µm axial resolution at 300 msec integration times per plane and orders of magnitude lower irradiation density than coherent Raman imaging schemes. We detail the apparatus and its performance, as well as its compatibility with fluorescence light-sheet and quantitative-phase imaging towards rapid and low phototoxicity multimodal imaging.},
doi = {10.1364/OE.435293},
journal = {Optics Express},
number = 20,
volume = 29,
place = {United States},
year = {Tue Sep 21 00:00:00 EDT 2021},
month = {Tue Sep 21 00:00:00 EDT 2021}
}
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Works referenced in this record:
Optical sectioning using single-plane-illumination Raman imaging
journal, September 2010
- Barman, Ishan; Tan, Khay Ming; Singh, Gajendra Pratap
- Journal of Raman Spectroscopy, Vol. 41, Issue 10
Performance of line-scan Raman microscopy for high-throughput chemical imaging of cell population
journal, January 2014
- Qi, Ji; Shih, Wei-Chuan
- Applied Optics, Vol. 53, Issue 13
Integrated one- and two-photon scanned oblique plane illumination (SOPi) microscopy for rapid volumetric imaging
journal, January 2018
- Kumar, Manish; Kishore, Sandeep; Nasenbeny, Jordan
- Optics Express, Vol. 26, Issue 10
Quantitative high-speed imaging of entire developing embryos with simultaneous multiview light-sheet microscopy
journal, June 2012
- Tomer, Raju; Khairy, Khaled; Amat, Fernando
- Nature Methods, Vol. 9, Issue 7
High-speed Raman imaging of cellular processes
journal, August 2016
- Ando, Jun; Palonpon, Almar F.; Sodeoka, Mikiko
- Current Opinion in Chemical Biology, Vol. 33
Resolution enhancement in a light-sheet-based microscope (SPIM)
journal, January 2006
- Engelbrecht, Christoph J.; Stelzer, Ernst H.
- Optics Letters, Vol. 31, Issue 10
Light sheet-excited spontaneous Raman imaging of a living fish by optical sectioning in a wide field Raman microscope
journal, January 2012
- Oshima, Yusuke; Sato, Hidetoshi; Kajiura-Kobayashi, Hiroko
- Optics Express, Vol. 20, Issue 15
Multi-purpose SLM-light-sheet microscope
journal, January 2018
- Garbellotto, Chiara; Taylor, Jonathan M.
- Biomedical Optics Express, Vol. 9, Issue 11
Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination
journal, March 2011
- Planchon, Thomas A.; Gao, Liang; Milkie, Daniel E.
- Nature Methods, Vol. 8, Issue 5
Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution
journal, October 2014
- Chen, Bi-Chang; Legant, Wesley R.; Wang, Kai
- Science, Vol. 346, Issue 6208
3D high- and super-resolution imaging using single-objective SPIM
journal, May 2015
- Galland, Remi; Grenci, Gianluca; Aravind, Ajay
- Nature Methods, Vol. 12, Issue 7
Highly inclined thin illumination enables clear single-molecule imaging in cells
journal, January 2008
- Tokunaga, Makio; Imamoto, Naoko; Sakata-Sogawa, Kumiko
- Nature Methods, Vol. 5, Issue 2
Spatial light interference microscopy (SLIM)
journal, January 2011
- Wang, Zhuo; Millet, Larry; Mir, Mustafa
- Optics Express, Vol. 19, Issue 2
Raman mixture composition and flow velocity imaging with high repetition rates
journal, January 2010
- Braeuer, Andreas; Engel, Sascha Ronald; Dowy, Stefan
- Optics Express, Vol. 18, Issue 24
In vivo lipidomics using single-cell Raman spectroscopy
journal, February 2011
- Wu, Huawen; Volponi, Joanne V.; Oliver, Ann E.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 9
Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering
journal, March 2014
- Wei, Lu; Hu, Fanghao; Shen, Yihui
- Nature Methods, Vol. 11, Issue 4
Epi-illumination SPIM for volumetric imaging with high spatial-temporal resolution
journal, May 2019
- Yang, Bin; Chen, Xingye; Wang, Yina
- Nature Methods, Vol. 16, Issue 6
Molecular-level pursuit of yeast mitosis by time- and space-resolved Raman spectroscopy
journal, January 2003
- Huang, Yu-San; Karashima, Takeshi; Yamamoto, Masayuki
- Journal of Raman Spectroscopy, Vol. 34, Issue 1
Experimental investigation of Bessel beam characteristics
journal, January 1992
- Lin, Y.; Seka, W.; Eberly, J. H.
- Applied Optics, Vol. 31, Issue 15
csiLSFM combines light-sheet fluorescence microscopy and coherent structured illumination for a lateral resolution below 100 nm
journal, April 2017
- Chang, Bo-Jui; Perez Meza, Victor Didier; Stelzer, Ernst H. K.
- Proceedings of the National Academy of Sciences, Vol. 114, Issue 19
Integrative quantitative-phase and airy light-sheet imaging
journal, November 2020
- Subedi, N. R.; Jung, P. S.; Bredeweg, E. L.
- Scientific Reports, Vol. 10, Issue 1
Quantum-enhanced nonlinear microscopy
journal, June 2021
- Casacio, Catxere A.; Madsen, Lars S.; Terrasson, Alex
- Nature, Vol. 594, Issue 7862
Optical Sectioning Deep Inside Live Embryos by Selective Plane Illumination Microscopy
journal, August 2004
- Huisken, J.; Swoger, J.; Del Bene, F.
- Science, Vol. 305, Issue 5686, p. 1007-1009
Observation of Accelerating Airy Beams
journal, November 2007
- Siviloglou, G. A.; Broky, J.; Dogariu, A.
- Physical Review Letters, Vol. 99, Issue 21
Deconvolution-free Subcellular Imaging with Axially Swept Light Sheet Microscopy
journal, June 2015
- Dean, Kevin M.; Roudot, Philippe; Welf, Erik S.
- Biophysical Journal, Vol. 108, Issue 12
Direct Visualization of De novo Lipogenesis in Single Living Cells
journal, October 2014
- Li, Junjie; Cheng, Ji-Xin
- Scientific Reports, Vol. 4, Issue 1
Raman and SERS microscopy for molecular imaging of live cells
journal, March 2013
- Palonpon, Almar F.; Ando, Jun; Yamakoshi, Hiroyuki
- Nature Protocols, Vol. 8, Issue 4
Thermal illumination limits in 3D Raman microscopy: A comparison of different sample illumination strategies to obtain maximum imaging speed
journal, August 2019
- Hauswald, Walter; Förster, Ronny; Popp, Jürgen
- PLOS ONE, Vol. 14, Issue 8
Imaging with a small number of photons
journal, January 2015
- Morris, Peter A.; Aspden, Reuben S.; Bell, Jessica E. C.
- Nature Communications, Vol. 6, Issue 1
Light sheet Raman micro-spectroscopy
journal, January 2016
- Müller, Walter; Kielhorn, Martin; Schmitt, Michael
- Optica, Vol. 3, Issue 4
Light Sheet Direct Raman Imaging Technique for Observation of Mixing of Solvents
journal, October 2009
- Oshima, Yusuke; Furihata, Chie; Sato, Hidetoshi
- Applied Spectroscopy, Vol. 63, Issue 10
Selective imaging of saturated and unsaturated lipids by wide-field CARS-microscopy
journal, January 2008
- Heinrich, Christoph; Hofer, Alexander; Ritsch, Andreas
- Optics Express, Vol. 16, Issue 4
A guide to light-sheet fluorescence microscopy for multiscale imaging
journal, April 2017
- Power, Rory M.; Huisken, Jan
- Nature Methods, Vol. 14, Issue 4
Rapid spontaneous Raman light sheet microscopy using cw-lasers and tunable filters
journal, January 2015
- Rocha-Mendoza, Israel; Licea-Rodriguez, Jacob; Marro, Mónica
- Biomedical Optics Express, Vol. 6, Issue 9
Simple Ciprofloxacin Resistance Test and Determination of Minimal Inhibitory Concentration within 2 h Using Raman Spectroscopy
journal, January 2018
- Kirchhoff, Johanna; Glaser, Uwe; Bohnert, Jürgen A.
- Analytical Chemistry, Vol. 90, Issue 3
3D single-molecule super-resolution microscopy with a tilted light sheet
journal, January 2018
- Gustavsson, Anna-Karin; Petrov, Petar N.; Lee, Maurice Y.
- Nature Communications, Vol. 9, Issue 1
Integrated single- and two-photon light sheet microscopy using accelerating beams
journal, May 2017
- Piksarv, Peeter; Marti, Dominik; Le, Tuan
- Scientific Reports, Vol. 7, Issue 1
Rapid Antibiotic Susceptibility Testing of Pathogenic Bacteria Using Heavy-Water-Labeled Single-Cell Raman Spectroscopy in Clinical Samples
journal, April 2019
- Yang, Kai; Li, Hong-Zhe; Zhu, Xuan
- Analytical Chemistry, Vol. 91, Issue 9
A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics
journal, November 2020
- Sapoznik, Etai; Chang, Bo-Jui; Huh, Jaewon
- eLife, Vol. 9
Light-sheet microscopy using an Airy beam
journal, April 2014
- Vettenburg, Tom; Dalgarno, Heather I. C.; Nylk, Jonathan
- Nature Methods, Vol. 11, Issue 5