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Title: Integrative quantitative-phase and airy light-sheet imaging

Abstract

Abstract Light-sheet microscopy enables considerable speed and phototoxicity gains, while quantitative-phase imaging confers label-free recognition of cells and organelles, and quantifies their number-density that, thermodynamically, is more representative of metabolism than size. Here, we report the fusion of these two imaging modalities onto a standard inverted microscope that retains compatibility with microfluidics and open-source software for image acquisition and processing. An accelerating Airy-beam light-sheet critically enabled imaging areas that were greater by more than one order of magnitude than a Gaussian beam illumination and matched exactly those of quantitative-phase imaging. Using this integrative imaging system, we performed a demonstrative multivariate investigation of live-cells in microfluidics that unmasked that cellular noise can affect the compartmental localization of metabolic reactions. We detail the design, assembly, and performance of the integrative imaging system, and discuss potential applications in biotechnology and evolutionary biology.

Authors:
; ; ; ; ; ;
Publication Date:
Research Org.:
Univ. of Idaho, Moscow, ID (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER); Polish Ministry of Higher Education
OSTI Identifier:
1720187
Alternate Identifier(s):
OSTI ID: 1724091
Grant/Contract Number:  
SC0019249
Resource Type:
Published Article
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Name: Scientific Reports Journal Volume: 10 Journal Issue: 1; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United Kingdom
Language:
English
Subject:
74 ATOMIC AND MOLECULAR PHYSICS

Citation Formats

Subedi, N. R., Jung, P. S., Bredeweg, E. L., Nemati, S., Baker, S. E., Christodoulides, D. N., and Vasdekis, A. E.. Integrative quantitative-phase and airy light-sheet imaging. United Kingdom: N. p., 2020. Web. https://doi.org/10.1038/s41598-020-76730-x.
Subedi, N. R., Jung, P. S., Bredeweg, E. L., Nemati, S., Baker, S. E., Christodoulides, D. N., & Vasdekis, A. E.. Integrative quantitative-phase and airy light-sheet imaging. United Kingdom. https://doi.org/10.1038/s41598-020-76730-x
Subedi, N. R., Jung, P. S., Bredeweg, E. L., Nemati, S., Baker, S. E., Christodoulides, D. N., and Vasdekis, A. E.. Thu . "Integrative quantitative-phase and airy light-sheet imaging". United Kingdom. https://doi.org/10.1038/s41598-020-76730-x.
@article{osti_1720187,
title = {Integrative quantitative-phase and airy light-sheet imaging},
author = {Subedi, N. R. and Jung, P. S. and Bredeweg, E. L. and Nemati, S. and Baker, S. E. and Christodoulides, D. N. and Vasdekis, A. E.},
abstractNote = {Abstract Light-sheet microscopy enables considerable speed and phototoxicity gains, while quantitative-phase imaging confers label-free recognition of cells and organelles, and quantifies their number-density that, thermodynamically, is more representative of metabolism than size. Here, we report the fusion of these two imaging modalities onto a standard inverted microscope that retains compatibility with microfluidics and open-source software for image acquisition and processing. An accelerating Airy-beam light-sheet critically enabled imaging areas that were greater by more than one order of magnitude than a Gaussian beam illumination and matched exactly those of quantitative-phase imaging. Using this integrative imaging system, we performed a demonstrative multivariate investigation of live-cells in microfluidics that unmasked that cellular noise can affect the compartmental localization of metabolic reactions. We detail the design, assembly, and performance of the integrative imaging system, and discuss potential applications in biotechnology and evolutionary biology.},
doi = {10.1038/s41598-020-76730-x},
journal = {Scientific Reports},
number = 1,
volume = 10,
place = {United Kingdom},
year = {2020},
month = {11}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1038/s41598-020-76730-x

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