Integrative Quantitative-Phase and Airy Light-Sheet Imaging

Subedi, Nava R.; Jung, Pawel S.; Bredeweg, Erin L.; Nemati, Shahla H.; Baker, Scott E.; Christodoulides, Demetrios N.; Vasdekis, Andreas E.

doi:10.1038/s41598-020-76730-x

Title: Integrative Quantitative-Phase and Airy Light-Sheet Imaging

Journal Article · Tue Dec 01 00:00:00 EST 2020 · Scientific Reports

DOI:https://doi.org/10.1038/s41598-020-76730-x· OSTI ID:1725826

Subedi, Nava R. ^[1]; Jung, Pawel S. ^[2];

^[3]; Nemati, Shahla H. ^[1];

^[3]; Christodoulides, Demetrios N. ^[2]; Vasdekis, Andreas E. ^[4]

University of Idaho
University of Central Florida
BATTELLE (PACIFIC NW LAB)
UNIVERSITY OF IDAHO

Light-sheet microscopy enables considerable speed and phototoxicity gains, while quantitative-phase imaging confers label-free organelle recognition and metabolic information that are inaccessible by conventional methods. We report the fusion of these two modalities onto a standard inverted microscope that retains compatibility with microfluidics. We describe the utilization of an accelerating Airy-beam light-sheet yielding identical imaging areas with interferometry, and an application in unmasking the effects of cellular noise on metabolic compartmentalization.

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Research Organization:: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-76RL01830

OSTI ID:: 1725826

Report Number(s):: PNNL-SA-153507

Journal Information:: Scientific Reports, Vol. 10

Country of Publication:: United States

Language:: English

References (61)

A guide to light-sheet fluorescence microscopy for multiscale imaging Power, Rory M.; Huisken, Jan Nature Methods, Vol. 14, Issue 4 https://doi.org/10.1038/nmeth.4224	journal	April 2017
Light-sheet microscopy: a tutorial Olarte, Omar E.; Andilla, Jordi; Gualda, Emilio J. Advances in Optics and Photonics, Vol. 10, Issue 1 https://doi.org/10.1364/AOP.10.000111	journal	January 2018
Optical Sectioning Deep Inside Live Embryos by Selective Plane Illumination Microscopy Huisken, J.; Swoger, J.; Del Bene, F. Science, Vol. 305, Issue 5686, p. 1007-1009 https://doi.org/10.1126/science.1100035	journal	August 2004
Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution Chen, Bi-Chang; Legant, Wesley R.; Wang, Kai Science, Vol. 346, Issue 6208 https://doi.org/10.1126/science.1257998	journal	October 2014
Light-sheet microscopy using an Airy beam Vettenburg, Tom; Dalgarno, Heather I. C.; Nylk, Jonathan Nature Methods, Vol. 11, Issue 5 https://doi.org/10.1038/nmeth.2922	journal	April 2014
Highly inclined thin illumination enables clear single-molecule imaging in cells Tokunaga, Makio; Imamoto, Naoko; Sakata-Sogawa, Kumiko Nature Methods, Vol. 5, Issue 2 https://doi.org/10.1038/nmeth1171	journal	January 2008
3D high- and super-resolution imaging using single-objective SPIM Galland, Remi; Grenci, Gianluca; Aravind, Ajay Nature Methods, Vol. 12, Issue 7 https://doi.org/10.1038/nmeth.3402	journal	May 2015
Epi-illumination SPIM for volumetric imaging with high spatial-temporal resolution Yang, Bin; Chen, Xingye; Wang, Yina Nature Methods, Vol. 16, Issue 6 https://doi.org/10.1038/s41592-019-0401-3	journal	May 2019
3D single-molecule super-resolution microscopy with a tilted light sheet Gustavsson, Anna-Karin; Petrov, Petar N.; Lee, Maurice Y. Nature Communications, Vol. 9, Issue 1 https://doi.org/10.1038/s41467-017-02563-4	journal	January 2018
Interference Microscopy and Mass Determination Barer, R. Nature, Vol. 169, Issue 4296 https://doi.org/10.1038/169366b0	journal	March 1952
Tomographic phase microscopy Choi, Wonshik; Fang-Yen, Christopher; Badizadegan, Kamran Nature Methods, Vol. 4, Issue 9 https://doi.org/10.1038/nmeth1078	journal	August 2007
Eliciting the impacts of cellular noise on metabolic trade-offs by quantitative mass imaging Vasdekis, A. E.; Alanazi, H.; Silverman, A. M. Nature Communications, Vol. 10, Issue 1 https://doi.org/10.1038/s41467-019-08717-w	journal	February 2019
Live-cell mass profiling: an emerging approach in quantitative biophysics Zangle, Thomas A.; Teitell, Michael A. Nature Methods, Vol. 11, Issue 12 https://doi.org/10.1038/nmeth.3175	journal	November 2014
Quantitative phase imaging in biomedicine Park, YongKeun; Depeursinge, Christian; Popescu, Gabriel Nature Photonics, Vol. 12, Issue 10 https://doi.org/10.1038/s41566-018-0253-x	journal	September 2018
PhaseStain: the digital staining of label-free quantitative phase microscopy images using deep learning Rivenson, Yair; Liu, Tairan; Wei, Zhensong Light: Science & Applications, Vol. 8, Issue 1 https://doi.org/10.1038/s41377-019-0129-y	journal	February 2019
Quantitative Phase Imaging and Artificial Intelligence: A Review Jo, YoungJu; Cho, Hyungjoo; Lee, Sang Yun IEEE Journal of Selected Topics in Quantum Electronics, Vol. 25, Issue 1 https://doi.org/10.1109/JSTQE.2018.2859234	journal	January 2019
Triacylglycerol Synthesis Enzymes Mediate Lipid Droplet Growth by Relocalizing from the ER to Lipid Droplets Wilfling, Florian; Wang, Huajin; Haas, Joel T. Developmental Cell, Vol. 24, Issue 4 https://doi.org/10.1016/j.devcel.2013.01.013	journal	February 2013
Swept confocally-aligned planar excitation (SCAPE) microscopy for high-speed volumetric imaging of behaving organisms Bouchard, Matthew B.; Voleti, Venkatakaushik; Mendes, César S. Nature Photonics, Vol. 9, Issue 2 https://doi.org/10.1038/nphoton.2014.323	journal	January 2015
Integrated one- and two-photon scanned oblique plane illumination (SOPi) microscopy for rapid volumetric imaging Kumar, Manish; Kishore, Sandeep; Nasenbeny, Jordan Optics Express, Vol. 26, Issue 10 https://doi.org/10.1364/OE.26.013027	journal	January 2018
Stochasticity of metabolism and growth at the single-cell level Kiviet, Daniel J.; Nghe, Philippe; Walker, Noreen Nature, Vol. 514, Issue 7522 https://doi.org/10.1038/nature13582	journal	September 2014
Spatial light interference microscopy (SLIM) Wang, Zhuo; Millet, Larry; Mir, Mustafa Optics Express, Vol. 19, Issue 2 https://doi.org/10.1364/OE.19.001016	journal	January 2011
Optical measurement of cycle-dependent cell growth Mir, Mustafa; Wang, Zhuo; Shen, Zhen Proceedings of the National Academy of Sciences, Vol. 108, Issue 32 https://doi.org/10.1073/pnas.1100506108	journal	July 2011
Observation of Accelerating Airy Beams Siviloglou, G. A.; Broky, J.; Dogariu, A. Physical Review Letters, Vol. 99, Issue 21 https://doi.org/10.1103/PhysRevLett.99.213901	journal	November 2007
sideSPIM – selective plane illumination based on a conventional inverted microscope Hedde, Per Niklas; Malacrida, Leonel; Ahrar, Siavash Biomedical Optics Express, Vol. 8, Issue 9 https://doi.org/10.1364/BOE.8.003918	journal	January 2017
Lipid production in Yarrowia lipolytica is maximized by engineering cytosolic redox metabolism Qiao, Kangjian; Wasylenko, Thomas M.; Zhou, Kang Nature Biotechnology, Vol. 35, Issue 2 https://doi.org/10.1038/nbt.3763	journal	January 2017
Harnessing Yarrowia lipolytica lipogenesis to create a platform for lipid and biofuel production Blazeck, John; Hill, Andrew; Liu, Leqian Nature Communications, Vol. 5, Issue 1 https://doi.org/10.1038/ncomms4131	journal	January 2014
Yarrowia lipolytica as a biotechnological chassis to produce usual and unusual fatty acids Ledesma-Amaro, Rodrigo; Nicaud, Jean-Marc Progress in Lipid Research, Vol. 61 https://doi.org/10.1016/j.plipres.2015.12.001	journal	January 2016
Regulation of amino-acid metabolism controls flux to lipid accumulation in Yarrowia lipolytica Kerkhoven, Eduard J.; Pomraning, Kyle R.; Baker, Scott E. npj Systems Biology and Applications, Vol. 2, Issue 1 https://doi.org/10.1038/npjsba.2016.5	journal	March 2016
Biodiesel fuel production by transesterification of oils Fukuda, Hideki; Kondo, Akihiko; Noda, Hideo Journal of Bioscience and Bioengineering, Vol. 92, Issue 5 https://doi.org/10.1016/S1389-1723(01)80288-7	journal	January 2001
The biophysics and cell biology of lipid droplets Thiam, Abdou Rachid; Farese Jr, Robert V.; Walther, Tobias C. Nature Reviews Molecular Cell Biology, Vol. 14, Issue 12 https://doi.org/10.1038/nrm3699	journal	November 2013
The life cycle of lipid droplets Hashemi, Hayaa F.; Goodman, Joel M. Current Opinion in Cell Biology, Vol. 33 https://doi.org/10.1016/j.ceb.2015.02.002	journal	April 2015
Lipid droplets: a unified view of a dynamic organelle Martin, Sally; Parton, Robert G. Nature Reviews Molecular Cell Biology, Vol. 7, Issue 5 https://doi.org/10.1038/nrm1912	journal	March 2006
Lipid droplets, lipophagy, and beyond Wang, Chao-Wen Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Vol. 1861, Issue 8 https://doi.org/10.1016/j.bbalip.2015.12.010	journal	August 2016
Lipid droplet hijacking by intracellular pathogens: Lipid droplet hijacking by intracellular pathogens Roingeard, Philippe; Melo, Rossana C. N. Cellular Microbiology, Vol. 19, Issue 1 https://doi.org/10.1111/cmi.12688	journal	November 2016
The lipid droplet is an important organelle for hepatitis C virus production Miyanari, Yusuke; Atsuzawa, Kimie; Usuda, Nobuteru Nature Cell Biology, Vol. 9, Issue 9 https://doi.org/10.1038/ncb1631	journal	August 2007
Lipid droplets: platforms with multiple functions in cancer hallmarks Cruz, André L. S.; Barreto, Ester de A.; Fazolini, Narayana P. B. Cell Death & Disease, Vol. 11, Issue 2 https://doi.org/10.1038/s41419-020-2297-3	journal	February 2020
Lipid Droplets Control the Maternal Histone Supply of Drosophila Embryos Li, Zhihuan; Thiel, Katharina; Thul, Peter J. Current Biology, Vol. 22, Issue 22 https://doi.org/10.1016/j.cub.2012.09.018	journal	November 2012
The Lipid-Droplet Proteome Reveals that Droplets Are a Protein-Storage Depot Cermelli, Silvia; Guo, Yi; Gross, Steven P. Current Biology, Vol. 16, Issue 18 https://doi.org/10.1016/j.cub.2006.07.062	journal	September 2006
Fat-specific protein 27 modulates nuclear factor of activated T cells 5 and the cellular response to stress Ueno, Masami; Shen, Wen-Jun; Patel, Shailja Journal of Lipid Research, Vol. 54, Issue 3 https://doi.org/10.1194/jlr.M033365	journal	December 2012
A lipid-based model for the creation of an escape hatch from the endoplasmic reticulum Ploegh, Hidde L. Nature, Vol. 448, Issue 7152 https://doi.org/10.1038/nature06004	journal	July 2007
A molecular genetic toolbox for Yarrowia lipolytica Bredeweg, Erin L.; Pomraning, Kyle R.; Dai, Ziyu Biotechnology for Biofuels, Vol. 10, Issue 1 https://doi.org/10.1186/s13068-016-0687-7	journal	January 2017
Robust microbial cell segmentation by optical-phase thresholding with minimal processing requirements: Robust Microbial Cell Segmentation Alanazi, H.; Canul, A. J.; Garman, A. Cytometry Part A, Vol. 91, Issue 5 https://doi.org/10.1002/cyto.a.23099	journal	March 2017
Three-dimensional label-free imaging and quantification of lipid droplets in live hepatocytes Kim, Kyoohyun; Lee, SeoEun; Yoon, Jonghee Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep36815	journal	November 2016
A Yeast Strain Lacking Lipid Particles Bears a Defect in Ergosterol Formation Sorger, Daniel; Athenstaedt, Karin; Hrastnik, Claudia Journal of Biological Chemistry, Vol. 279, Issue 30 https://doi.org/10.1074/jbc.M403251200	journal	May 2004
The endosomal sorting complex required for transport complex negatively regulates Erg6 degradation under specific glucose restriction conditions Zhang, Ao; Meng, Ying; Li, Qunli Traffic, Vol. 21, Issue 7 https://doi.org/10.1111/tra.12732	journal	June 2020
Stationary phase lipophagy as a cellular mechanism to recycle sterols during quiescence Wang, Chao-Wen Autophagy, Vol. 10, Issue 11 https://doi.org/10.4161/auto.36137	journal	October 2014
Interdependence of Cell Growth and Gene Expression: Origins and Consequences Scott, M.; Gunderson, C. W.; Mateescu, E. M. Science, Vol. 330, Issue 6007 https://doi.org/10.1126/science.1192588	journal	November 2010
Stochastic Gene Expression in a Single Cell Elowitz, M. B. Science, Vol. 297, Issue 5584 https://doi.org/10.1126/science.1070919	journal	August 2002
Stochastic protein expression in individual cells at the single molecule level Cai, Long; Friedman, Nir; Xie, X. Sunney Nature, Vol. 440, Issue 7082 https://doi.org/10.1038/nature04599	journal	March 2006
Nature, Nurture, or Chance: Stochastic Gene Expression and Its Consequences Raj, Arjun; van Oudenaarden, Alexander Cell, Vol. 135, Issue 2 https://doi.org/10.1016/j.cell.2008.09.050	journal	October 2008
Stochasticity in gene expression: from theories to phenotypes Kærn, Mads; Elston, Timothy C.; Blake, William J. Nature Reviews Genetics, Vol. 6, Issue 6 https://doi.org/10.1038/nrg1615	journal	May 2005
Bacterial Persistence as a Phenotypic Switch Balaban, Nathalie Q.; Merrin, Jack; Chait, Remy Science, Vol. 305, Issue 5690, p. 1622-1625 https://doi.org/10.1126/science.1099390	journal	September 2004
Single-cell genome sequencing: current state of the science Gawad, Charles; Koh, Winston; Quake, Stephen R. Nature Reviews Genetics, Vol. 17, Issue 3 https://doi.org/10.1038/nrg.2015.16	journal	January 2016
Multiplexed quantification of proteins and transcripts in single cells Peterson, Vanessa M.; Zhang, Kelvin Xi; Kumar, Namit Nature Biotechnology, Vol. 35, Issue 10 https://doi.org/10.1038/nbt.3973	journal	August 2017
Single-Cell Metabolomics: Analytical and Biological Perspectives Zenobi, R. Science, Vol. 342, Issue 6163 https://doi.org/10.1126/science.1243259	journal	December 2013
Gradient light interference microscopy for 3D imaging of unlabeled specimens Nguyen, Tan H.; Kandel, Mikhail E.; Rubessa, Marcello Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/s41467-017-00190-7	journal	August 2017
High-fidelity optical diffraction tomography of multiple scattering samples Lim, Joowon; Ayoub, Ahmed B.; Antoine, Elizabeth E. Light: Science & Applications, Vol. 8, Issue 1 https://doi.org/10.1038/s41377-019-0195-1	journal	September 2019
Advanced methods of microscope control using μManager software Edelstein, Arthur D.; Tsuchida, Mark A.; Amodaj, Nenad Journal of Biological Methods, Vol. 1, Issue 2 https://doi.org/10.14440/jbm.2014.36	journal	July 2014
DeconvolutionLab2: An open-source software for deconvolution microscopy Sage, Daniel; Donati, Lauréne; Soulez, Ferréol Methods, Vol. 115 https://doi.org/10.1016/j.ymeth.2016.12.015	journal	February 2017
Ergosterol Biosynthesis: a Fungal Pathway for life on Land? Dupont, Sebastien; Lemetais, Guillaume; Ferreira, Thierry Evolution, Vol. 66, Issue 9 https://doi.org/10.1111/j.1558-5646.2012.01667.x	journal	May 2012
NIH Image to ImageJ: 25 years of image analysis Schneider, Caroline A.; Rasband, Wayne S.; Eliceiri, Kevin W. Nature Methods, Vol. 9, Issue 7 https://doi.org/10.1038/nmeth.2089	journal	June 2012

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