Proximity-based proteomics reveals the thylakoid lumen proteome in the cyanobacterium Synechococcus sp. PCC 7002
Abstract
Abstract Cyanobacteria possess unique intracellular organization. Many proteomic studies have examined different features of cyanobacteria to learn about the intracellular structures and their respective functions. While these studies have made great progress in understanding cyanobacterial physiology, the conventional fractionation methods used to purify cellular structures have limitations; specifically, certain regions of cells cannot be purified with existing fractionation methods. Proximity-based proteomics techniques were developed to overcome the limitations of biochemical fractionation for proteomics. Proximity-based proteomics relies on spatiotemporal protein labeling followed by mass spectrometry of the labeled proteins to determine the proteome of the region of interest. We performed proximity-based proteomics in the cyanobacterium Synechococcus sp. PCC 7002 with the APEX2 enzyme, an engineered ascorbate peroxidase. We determined the proteome of the thylakoid lumen, a region of the cell that has remained challenging to study with existing methods, using a translational fusion between APEX2 and PsbU, a lumenal subunit of photosystem II. Our results demonstrate the power of APEX2 as a tool to study the cell biology of intracellular features and processes, including photosystem II assembly in cyanobacteria, with enhanced spatiotemporal resolution.
- Publication Date:
- Research Org.:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF); National Institutes of Health (NIH)
- OSTI Identifier:
- 1734387
- Alternate Identifier(s):
- OSTI ID: 1755713
- Report Number(s):
- NREL/JA-2800-78686
Journal ID: ISSN 0166-8595; PII: 806
- Grant/Contract Number:
- SC0019306; AC36-08GO28308; 1144807; S10-OD025267
- Resource Type:
- Published Article
- Journal Name:
- Photosynthesis Research
- Additional Journal Information:
- Journal Name: Photosynthesis Research Journal Volume: 147 Journal Issue: 2; Journal ID: ISSN 0166-8595
- Publisher:
- Springer
- Country of Publication:
- Netherlands
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; APEX2; proximity-based proteomics; thylakoid lumen; photosynthesis; photosystem II; cyanobacteria
Citation Formats
Dahlgren, Kelsey K., Gates, Colin, Lee, Thomas, and Cameron, Jeffrey C. Proximity-based proteomics reveals the thylakoid lumen proteome in the cyanobacterium Synechococcus sp. PCC 7002. Netherlands: N. p., 2020.
Web. doi:10.1007/s11120-020-00806-y.
Dahlgren, Kelsey K., Gates, Colin, Lee, Thomas, & Cameron, Jeffrey C. Proximity-based proteomics reveals the thylakoid lumen proteome in the cyanobacterium Synechococcus sp. PCC 7002. Netherlands. https://doi.org/10.1007/s11120-020-00806-y
Dahlgren, Kelsey K., Gates, Colin, Lee, Thomas, and Cameron, Jeffrey C. Sun .
"Proximity-based proteomics reveals the thylakoid lumen proteome in the cyanobacterium Synechococcus sp. PCC 7002". Netherlands. https://doi.org/10.1007/s11120-020-00806-y.
@article{osti_1734387,
title = {Proximity-based proteomics reveals the thylakoid lumen proteome in the cyanobacterium Synechococcus sp. PCC 7002},
author = {Dahlgren, Kelsey K. and Gates, Colin and Lee, Thomas and Cameron, Jeffrey C.},
abstractNote = {Abstract Cyanobacteria possess unique intracellular organization. Many proteomic studies have examined different features of cyanobacteria to learn about the intracellular structures and their respective functions. While these studies have made great progress in understanding cyanobacterial physiology, the conventional fractionation methods used to purify cellular structures have limitations; specifically, certain regions of cells cannot be purified with existing fractionation methods. Proximity-based proteomics techniques were developed to overcome the limitations of biochemical fractionation for proteomics. Proximity-based proteomics relies on spatiotemporal protein labeling followed by mass spectrometry of the labeled proteins to determine the proteome of the region of interest. We performed proximity-based proteomics in the cyanobacterium Synechococcus sp. PCC 7002 with the APEX2 enzyme, an engineered ascorbate peroxidase. We determined the proteome of the thylakoid lumen, a region of the cell that has remained challenging to study with existing methods, using a translational fusion between APEX2 and PsbU, a lumenal subunit of photosystem II. Our results demonstrate the power of APEX2 as a tool to study the cell biology of intracellular features and processes, including photosystem II assembly in cyanobacteria, with enhanced spatiotemporal resolution.},
doi = {10.1007/s11120-020-00806-y},
journal = {Photosynthesis Research},
number = 2,
volume = 147,
place = {Netherlands},
year = {Sun Dec 06 00:00:00 EST 2020},
month = {Sun Dec 06 00:00:00 EST 2020}
}
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