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Title: Effect of mono- and dichromatic light quality on growth rates and photosynthetic performance of Synechococcus sp. PCC 7002

Abstract

Synechococcus sp. PCC 7002 was grown to steady state in optically thin turbidostat cultures under conditions for which light quantity and quality was systematically varied by modulating the output of narrow-band LEDs. Cells were provided photons absorbed primarily by chlorophyll (680 nm) or phycocyanin (630 nm) as the organism was subjected to four distinct mono- and dichromatic regimes. During cultivation with dichromatic light, growth rates displayed by Synechococcus sp. PCC 7002 were generally proportional to the total incident irradiance at values < 275 µmol photons m-2 s-1 and were not affected by the ratio of 630:680 nm wavelengths. Notably, under monochromatic light conditions, cultures exhibited similar growth rates only when they were irradiated with 630 nm light; cultures irradiated with only 680 nm light grew at rates that were 60 – 70% of those under other light quality regimes at equivalent irradiances. The functionality of photosystem II and associated processes such as maximum rate of photosynthetic electron transport, rate of cyclic electron flow, and rate of dark respiration generally increased as a function of growth rate. Nonetheless, some of the photophysiological parameters measured here displayed distinct patterns with respect to growth rate of cultures adapted to a single wavelength includingmore » phycobiliprotein content, which increased under severely light-limited growth conditions. Additionally, the ratio of photosystem II to photosystem I increased approximately 40% over the range of growth rates, although cells grown with 680 nm light only had the highest ratios. These results suggest the presence of effective mechanisms which allow acclimation of Synechococcus sp. PCC 7002 acclimation to different irradiance conditions.« less

Authors:
; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1170060
Report Number(s):
PNNL-SA-101364
KP1601010
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Frontiers in Microbiology, 5:Article No. 488
Additional Journal Information:
Journal Name: Frontiers in Microbiology, 5:Article No. 488
Country of Publication:
United States
Language:
English
Subject:
CYANOBACTERIA; PHOTOSYNTHESIS; CHLOROPHYLL; PHYCOBILIPROTEINS; FLUORESCENCE; cyanobacteria; photosynthesis; chlorophyll; phycobiliprotein; turbidostat; fluorescence

Citation Formats

Bernstein, Hans C., Konopka, Allan, Melnicki, Matthew R., Hill, Eric A., Kucek, Leo A., Zhang, Shuyi, Shen, Gaozhong, Bryant, Donald A., and Beliaev, Alex S. Effect of mono- and dichromatic light quality on growth rates and photosynthetic performance of Synechococcus sp. PCC 7002. United States: N. p., 2014. Web. doi:10.3389/fmicb.2014.00488.
Bernstein, Hans C., Konopka, Allan, Melnicki, Matthew R., Hill, Eric A., Kucek, Leo A., Zhang, Shuyi, Shen, Gaozhong, Bryant, Donald A., & Beliaev, Alex S. Effect of mono- and dichromatic light quality on growth rates and photosynthetic performance of Synechococcus sp. PCC 7002. United States. doi:10.3389/fmicb.2014.00488.
Bernstein, Hans C., Konopka, Allan, Melnicki, Matthew R., Hill, Eric A., Kucek, Leo A., Zhang, Shuyi, Shen, Gaozhong, Bryant, Donald A., and Beliaev, Alex S. Fri . "Effect of mono- and dichromatic light quality on growth rates and photosynthetic performance of Synechococcus sp. PCC 7002". United States. doi:10.3389/fmicb.2014.00488.
@article{osti_1170060,
title = {Effect of mono- and dichromatic light quality on growth rates and photosynthetic performance of Synechococcus sp. PCC 7002},
author = {Bernstein, Hans C. and Konopka, Allan and Melnicki, Matthew R. and Hill, Eric A. and Kucek, Leo A. and Zhang, Shuyi and Shen, Gaozhong and Bryant, Donald A. and Beliaev, Alex S.},
abstractNote = {Synechococcus sp. PCC 7002 was grown to steady state in optically thin turbidostat cultures under conditions for which light quantity and quality was systematically varied by modulating the output of narrow-band LEDs. Cells were provided photons absorbed primarily by chlorophyll (680 nm) or phycocyanin (630 nm) as the organism was subjected to four distinct mono- and dichromatic regimes. During cultivation with dichromatic light, growth rates displayed by Synechococcus sp. PCC 7002 were generally proportional to the total incident irradiance at values < 275 µmol photons m-2 s-1 and were not affected by the ratio of 630:680 nm wavelengths. Notably, under monochromatic light conditions, cultures exhibited similar growth rates only when they were irradiated with 630 nm light; cultures irradiated with only 680 nm light grew at rates that were 60 – 70% of those under other light quality regimes at equivalent irradiances. The functionality of photosystem II and associated processes such as maximum rate of photosynthetic electron transport, rate of cyclic electron flow, and rate of dark respiration generally increased as a function of growth rate. Nonetheless, some of the photophysiological parameters measured here displayed distinct patterns with respect to growth rate of cultures adapted to a single wavelength including phycobiliprotein content, which increased under severely light-limited growth conditions. Additionally, the ratio of photosystem II to photosystem I increased approximately 40% over the range of growth rates, although cells grown with 680 nm light only had the highest ratios. These results suggest the presence of effective mechanisms which allow acclimation of Synechococcus sp. PCC 7002 acclimation to different irradiance conditions.},
doi = {10.3389/fmicb.2014.00488},
journal = {Frontiers in Microbiology, 5:Article No. 488},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {9}
}