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Title: Preventing Bio-Bloopers and XFEL Follies: Best Practices from your Friendly Instrument Staff

Abstract

Serial Femtosecond Crystallography (SFX) at X-ray Free electron Lasers (XFELs) is a relatively new field promising to deliver unparalleled spatial and temporal resolution on biological systems and there dynamics. Over the past decade, though, there have been a handful of results that have truly delivered on these promises. Why? SFX has many paradigm shifting techniques not seen in typical structural biology arenas, such as creating a concentrated slurry of microcrystals rather than a handful of loopable prisms worthy of a catalog photo. Then taking that slurry and high speed jetting them towards the vacuum or helium interation region to destroy less than 1% of your sample and waste the other 99. The literature is full of techniques and methods promising to cure what ails your experiment, yet as an instrument scientist will tell you –and a first author might admit after a few drinks at the conference happy hour—is that there are a lot more failures than the success we published, results may vary. We will walk through a best practices on how to prepare your sample and chose a sample delivery technique that will amerliorate your studies rather than undermine your hardwork and hopefully lead to better experimental planningmore » and execution, inching you closer to that scientific goal and that call from Stockholm. This will be written in a more editorialized fashion and is meant to give the reader an idea of what to try or how they should be thinking. Welcome to SFX, now what?« less

Authors:
ORCiD logo [1]; ORCiD logo [1]
  1. SLAC National Accelerator Lab., Menlo Park, CA (United States)
Publication Date:
Research Org.:
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1630899
Grant/Contract Number:  
AC02-76SF00515
Resource Type:
Accepted Manuscript
Journal Name:
Crystals
Additional Journal Information:
Journal Volume: 10; Journal Issue: 4; Journal ID: ISSN 2073-4352
Publisher:
MDPI
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION; serial femtosecond crystalography; x-ray free electron laser; sample delivery; sample preparation; new users

Citation Formats

Kupitz, Christopher, and Sierra, Raymond G. Preventing Bio-Bloopers and XFEL Follies: Best Practices from your Friendly Instrument Staff. United States: N. p., 2020. Web. doi:10.3390/cryst10040251.
Kupitz, Christopher, & Sierra, Raymond G. Preventing Bio-Bloopers and XFEL Follies: Best Practices from your Friendly Instrument Staff. United States. https://doi.org/10.3390/cryst10040251
Kupitz, Christopher, and Sierra, Raymond G. Fri . "Preventing Bio-Bloopers and XFEL Follies: Best Practices from your Friendly Instrument Staff". United States. https://doi.org/10.3390/cryst10040251. https://www.osti.gov/servlets/purl/1630899.
@article{osti_1630899,
title = {Preventing Bio-Bloopers and XFEL Follies: Best Practices from your Friendly Instrument Staff},
author = {Kupitz, Christopher and Sierra, Raymond G.},
abstractNote = {Serial Femtosecond Crystallography (SFX) at X-ray Free electron Lasers (XFELs) is a relatively new field promising to deliver unparalleled spatial and temporal resolution on biological systems and there dynamics. Over the past decade, though, there have been a handful of results that have truly delivered on these promises. Why? SFX has many paradigm shifting techniques not seen in typical structural biology arenas, such as creating a concentrated slurry of microcrystals rather than a handful of loopable prisms worthy of a catalog photo. Then taking that slurry and high speed jetting them towards the vacuum or helium interation region to destroy less than 1% of your sample and waste the other 99. The literature is full of techniques and methods promising to cure what ails your experiment, yet as an instrument scientist will tell you –and a first author might admit after a few drinks at the conference happy hour—is that there are a lot more failures than the success we published, results may vary. We will walk through a best practices on how to prepare your sample and chose a sample delivery technique that will amerliorate your studies rather than undermine your hardwork and hopefully lead to better experimental planning and execution, inching you closer to that scientific goal and that call from Stockholm. This will be written in a more editorialized fashion and is meant to give the reader an idea of what to try or how they should be thinking. Welcome to SFX, now what?},
doi = {10.3390/cryst10040251},
journal = {Crystals},
number = 4,
volume = 10,
place = {United States},
year = {2020},
month = {3}
}