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Title: Surface Water Microbial Community Response to the Biocide 2,2-Dibromo-3-Nitrilopropionamide, Used in Unconventional Oil and Gas Extraction

Abstract

Production of unconventional oil and gas continues to rise, but the effects of high-density hydraulic fracturing (HF) activity near aquatic ecosystems are not fully understood. A commonly used biocide in HF, 2,2-dibromo-3-nitrilopropionamide (DBNPA), was studied in microcosms of HF-impacted (HF+) versus HF-unimpacted (HF-) surface water streams to (i) compare the microbial community response, (ii) investigate DBNPA degradation products based on past HF exposure, and (iii) compare the microbial community response differences and similarities between the HF biocides DBNPA and glutaraldehyde. The microbial community responded to DBNPA differently in HF-impacted versus HF-unimpacted microcosms in terms of the number of 16S rRNA gene copies quantified, alpha and beta diversity, and differential abundance analyses of microbial community composition through time. The differences in microbial community changes affected degradation dynamics. HF-impacted microbial communities were more sensitive to DBNPA, causing the biocide and by-products of the degradation to persist for longer than in HF-unimpacted microcosms. A total of 17 DBNPA by-products were detected, many of them not widely known as DBNPA by-products. Many of the brominated by-products detected that are believed to be uncharacterized may pose environmental and health impacts. Similar taxa were able to tolerate glutaraldehyde and DBNPA; however, DBNPA was not as effectivemore » for microbial control, as indicated by a smaller overall decrease of 16S rRNA gene copies/ml after exposure to the biocide, and a more diverse set of taxa was able to tolerate it. These findings suggest that past HF activity in streams can affect the microbial community response to environmental perturbation such as that caused by the biocide DBNPA.« less

Authors:
 [1]; ORCiD logo [2];  [1];  [3];  [4];  [4];  [4];  [5];  [5];  [1];  [5]; ORCiD logo [6]
+ Show Author Affiliations
  1. Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  2. Michigan Technological Univ., Houghton, MI (United States)
  3. Chinese Academy of Sciences (CAS), Liaoning, Shenyang (China). Key Lab. of Pollution Ecology and Environmental Engineering, Inst. of Applied Ecology; Univ. of Tennessee, Knoxville, TN (United States)
  4. Univ. of Tennessee, Knoxville, TN (United States)
  5. Juniata College, Huntingdon, PA (United States)
  6. Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Inst. for a Secure and Sustainable Environment, Knoxville, TN (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC); National Science Foundation (NSF); Methane Center in the Institute for a Secure and Sustainable Environment
OSTI Identifier:
1580966
Alternate Identifier(s):
OSTI ID: 1608200
Grant/Contract Number:  
AC02-05CH11231; AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
Applied and Environmental Microbiology
Additional Journal Information:
Journal Volume: 85; Journal Issue: 21; Journal ID: ISSN 0099-2240
Publisher:
American Society for Microbiology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Campa, Maria Fernanda, Techtmann, Stephen M., Ladd, Mallory P., Yan, Jun, Patterson, Megan, Garcia de Matos Amaral, Amanda, Carter, Kimberly E., Ulrich, Nikea, Grant, Christopher J., Hettich, Robert L., Lamendella, Regina, and Hazen, Terry C. Surface Water Microbial Community Response to the Biocide 2,2-Dibromo-3-Nitrilopropionamide, Used in Unconventional Oil and Gas Extraction. United States: N. p., 2019. Web. doi:10.1128/AEM.01336-19.
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Campa, Maria Fernanda, Techtmann, Stephen M., Ladd, Mallory P., Yan, Jun, Patterson, Megan, Garcia de Matos Amaral, Amanda, Carter, Kimberly E., Ulrich, Nikea, Grant, Christopher J., Hettich, Robert L., Lamendella, Regina, & Hazen, Terry C. Surface Water Microbial Community Response to the Biocide 2,2-Dibromo-3-Nitrilopropionamide, Used in Unconventional Oil and Gas Extraction. United States. https://doi.org/10.1128/AEM.01336-19
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Campa, Maria Fernanda, Techtmann, Stephen M., Ladd, Mallory P., Yan, Jun, Patterson, Megan, Garcia de Matos Amaral, Amanda, Carter, Kimberly E., Ulrich, Nikea, Grant, Christopher J., Hettich, Robert L., Lamendella, Regina, and Hazen, Terry C. Fri . "Surface Water Microbial Community Response to the Biocide 2,2-Dibromo-3-Nitrilopropionamide, Used in Unconventional Oil and Gas Extraction". United States. https://doi.org/10.1128/AEM.01336-19. https://www.osti.gov/servlets/purl/1580966.
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@article{osti_1580966,
title = {Surface Water Microbial Community Response to the Biocide 2,2-Dibromo-3-Nitrilopropionamide, Used in Unconventional Oil and Gas Extraction},
author = {Campa, Maria Fernanda and Techtmann, Stephen M. and Ladd, Mallory P. and Yan, Jun and Patterson, Megan and Garcia de Matos Amaral, Amanda and Carter, Kimberly E. and Ulrich, Nikea and Grant, Christopher J. and Hettich, Robert L. and Lamendella, Regina and Hazen, Terry C.},
abstractNote = {Production of unconventional oil and gas continues to rise, but the effects of high-density hydraulic fracturing (HF) activity near aquatic ecosystems are not fully understood. A commonly used biocide in HF, 2,2-dibromo-3-nitrilopropionamide (DBNPA), was studied in microcosms of HF-impacted (HF+) versus HF-unimpacted (HF-) surface water streams to (i) compare the microbial community response, (ii) investigate DBNPA degradation products based on past HF exposure, and (iii) compare the microbial community response differences and similarities between the HF biocides DBNPA and glutaraldehyde. The microbial community responded to DBNPA differently in HF-impacted versus HF-unimpacted microcosms in terms of the number of 16S rRNA gene copies quantified, alpha and beta diversity, and differential abundance analyses of microbial community composition through time. The differences in microbial community changes affected degradation dynamics. HF-impacted microbial communities were more sensitive to DBNPA, causing the biocide and by-products of the degradation to persist for longer than in HF-unimpacted microcosms. A total of 17 DBNPA by-products were detected, many of them not widely known as DBNPA by-products. Many of the brominated by-products detected that are believed to be uncharacterized may pose environmental and health impacts. Similar taxa were able to tolerate glutaraldehyde and DBNPA; however, DBNPA was not as effective for microbial control, as indicated by a smaller overall decrease of 16S rRNA gene copies/ml after exposure to the biocide, and a more diverse set of taxa was able to tolerate it. These findings suggest that past HF activity in streams can affect the microbial community response to environmental perturbation such as that caused by the biocide DBNPA.},
doi = {10.1128/AEM.01336-19},
journal = {Applied and Environmental Microbiology},
number = 21,
volume = 85,
place = {United States},
year = {Fri Aug 23 00:00:00 EDT 2019},
month = {Fri Aug 23 00:00:00 EDT 2019}
}
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https://doi.org/10.1128/AEM.01336-19
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