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Title: Impacts of Glutaraldehyde on Microbial Community Structure and Degradation Potential in Streams Impacted by Hydraulic Fracturing

Abstract

The environmental impacts of hydraulic fracturing, particularly those of surface spills in aquatic ecosystems, are not fully understood. The goals of this study were to (1) understand the effect of previous exposure to hydraulic fracturing fluids on aquatic microbial community structure and (2) examine the impacts exposure has on biodegradation potential of the biocide glutaraldehyde. Microcosms were constructed from hydraulic fracturing-impacted and nonhydraulic fracturing-impacted streamwater within the Marcellus shale region in Pennsylvania. Microcosms were amended with glutaraldehyde and incubated aerobically for 56 days. Microbial community adaptation to glutaraldehyde was monitored using 16S rRNA gene amplicon sequencing and quantification by qPCR. Abiotic and biotic glutaraldehyde degradation was measured using ultra-performance liquid chromatography--high resolution mass spectrometry and total organic carbon. It was found that nonhydraulic fracturing-impacted microcosms biodegraded glutaraldehyde faster than the hydraulic fracturing-impacted microcosms, showing a decrease in degradation potential after exposure to hydraulic fracturing activity. Hydraulic fracturing-impacted microcosms showed higher richness after glutaraldehyde exposure compared to unimpacted streams, indicating an increased tolerance to glutaraldehyde in hydraulic fracturing impacted streams. Beta diversity and differential abundance analysis of sequence count data showed different bacterial enrichment for hydraulic fracturing-impacted and nonhydraulic fracturing-impacted microcosms after glutaraldehyde addition. These findings demonstrated a lasting effect onmore » microbial community structure and glutaraldehyde degradation potential in streams impacted by hydraulic fracturing operations.« less

Authors:
 [1];  [2];  [3];  [3];  [3];  [3];  [4];  [3];  [4];  [4]; ORCiD logo [1]
  1. Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  2. Michigan Technological Univ., Houghton, MI (United States)
  3. Univ. of Tennessee, Knoxville, TN (United States)
  4. Juniata College, Huntingdon, PA (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1474525
Alternate Identifier(s):
OSTI ID: 1479407
Grant/Contract Number:  
AC05-00OR22725; AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
Environmental Science and Technology
Additional Journal Information:
Journal Volume: 52; Journal Issue: 10; Journal ID: ISSN 0013-936X
Publisher:
American Chemical Society (ACS)
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES

Citation Formats

Campa, Maria Fernanda, Techtmann, Stephen M., Gibson, Caleb M., Zhu, Xiaojuan, Patterson, Megan, Garcia de Matos Amaral, Amanda, Ulrich, Nikea, Campagna, Shawn R., Grant, Christopher J., Lamendella, Regina, and Hazen, Terry C. Impacts of Glutaraldehyde on Microbial Community Structure and Degradation Potential in Streams Impacted by Hydraulic Fracturing. United States: N. p., 2018. Web. doi:10.1021/acs.est.8b00239.
Campa, Maria Fernanda, Techtmann, Stephen M., Gibson, Caleb M., Zhu, Xiaojuan, Patterson, Megan, Garcia de Matos Amaral, Amanda, Ulrich, Nikea, Campagna, Shawn R., Grant, Christopher J., Lamendella, Regina, & Hazen, Terry C. Impacts of Glutaraldehyde on Microbial Community Structure and Degradation Potential in Streams Impacted by Hydraulic Fracturing. United States. doi:10.1021/acs.est.8b00239.
Campa, Maria Fernanda, Techtmann, Stephen M., Gibson, Caleb M., Zhu, Xiaojuan, Patterson, Megan, Garcia de Matos Amaral, Amanda, Ulrich, Nikea, Campagna, Shawn R., Grant, Christopher J., Lamendella, Regina, and Hazen, Terry C. Mon . "Impacts of Glutaraldehyde on Microbial Community Structure and Degradation Potential in Streams Impacted by Hydraulic Fracturing". United States. doi:10.1021/acs.est.8b00239. https://www.osti.gov/servlets/purl/1474525.
@article{osti_1474525,
title = {Impacts of Glutaraldehyde on Microbial Community Structure and Degradation Potential in Streams Impacted by Hydraulic Fracturing},
author = {Campa, Maria Fernanda and Techtmann, Stephen M. and Gibson, Caleb M. and Zhu, Xiaojuan and Patterson, Megan and Garcia de Matos Amaral, Amanda and Ulrich, Nikea and Campagna, Shawn R. and Grant, Christopher J. and Lamendella, Regina and Hazen, Terry C.},
abstractNote = {The environmental impacts of hydraulic fracturing, particularly those of surface spills in aquatic ecosystems, are not fully understood. The goals of this study were to (1) understand the effect of previous exposure to hydraulic fracturing fluids on aquatic microbial community structure and (2) examine the impacts exposure has on biodegradation potential of the biocide glutaraldehyde. Microcosms were constructed from hydraulic fracturing-impacted and nonhydraulic fracturing-impacted streamwater within the Marcellus shale region in Pennsylvania. Microcosms were amended with glutaraldehyde and incubated aerobically for 56 days. Microbial community adaptation to glutaraldehyde was monitored using 16S rRNA gene amplicon sequencing and quantification by qPCR. Abiotic and biotic glutaraldehyde degradation was measured using ultra-performance liquid chromatography--high resolution mass spectrometry and total organic carbon. It was found that nonhydraulic fracturing-impacted microcosms biodegraded glutaraldehyde faster than the hydraulic fracturing-impacted microcosms, showing a decrease in degradation potential after exposure to hydraulic fracturing activity. Hydraulic fracturing-impacted microcosms showed higher richness after glutaraldehyde exposure compared to unimpacted streams, indicating an increased tolerance to glutaraldehyde in hydraulic fracturing impacted streams. Beta diversity and differential abundance analysis of sequence count data showed different bacterial enrichment for hydraulic fracturing-impacted and nonhydraulic fracturing-impacted microcosms after glutaraldehyde addition. These findings demonstrated a lasting effect on microbial community structure and glutaraldehyde degradation potential in streams impacted by hydraulic fracturing operations.},
doi = {10.1021/acs.est.8b00239},
journal = {Environmental Science and Technology},
number = 10,
volume = 52,
place = {United States},
year = {2018},
month = {4}
}

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