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Title: Influence of Light on Particulate Organic Matter Utilization by Attached and Free-Living Marine Bacteria

Abstract

Light takes on a central role on primary productivity of aquatic systems. However, its potential impact on the degradation of photosynthetically produced biomass is not well understood. We investigated the patterns of light-induced particle breakdown and bacterial assimilation of detrital C and N using 13C and 15N labeled freeze-thawed diatom cells incubated in laboratory microcosms with a marine microbial community freshly collected from the Pacific Ocean. Particles incubated in the dark resulted in increased bacterial counts and dissolved organic carbon concentrations compared to those incubated in the light. Light also influenced the attached and free-living microbial community structure as detected by 16S rRNA gene amplicon sequencing. For example, Sphingobacteriia were enriched on dark-incubated particles and taxa from the family Flavobacteriaceae and the genus Pseudoalteromonas were numerically enriched on particles in the light. Isotope incorporation analysis by phylogenetic microarray and NanoSIMS (a method called Chip-SIP) identified free-living and attached microbial taxa able to incorporate N and C from the particles. Some taxa, including members of the Flavobacteriaceae and Cryomorphaceae, exhibited increased isotope incorporation in the light, suggesting the use of photoheterotrophic metabolisms. Yet, some members of Oceanospirillales and Rhodospirillales showed decreased isotope incorporation in the light, suggesting that their heterotrophic metabolism,more » particularly when occurring on particles, might increase at night or may be inhibited by sunlight. These results show that light influences particle degradation and C and N incorporation by attached bacteria, suggesting that the transfer between particulate and free-living phases are likely affected by external factors that change with the light regime, such as time of day, water column depth and season.« less

Authors:
 [1];  [2];  [3];  [2];  [3]
  1. Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE), Ensenada (Mexico); Univ. of Southern California, Los Angeles, CA (United States)
  2. Univ. of Southern California, Los Angeles, CA (United States)
  3. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE National Nuclear Security Administration (NNSA); USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); National Science Foundation (NSF)
OSTI Identifier:
1548317
Report Number(s):
LLNL-JRNL-767293
Journal ID: ISSN 1664-302X; 957764
Grant/Contract Number:  
AC52-07NA27344
Resource Type:
Accepted Manuscript
Journal Name:
Frontiers in Microbiology
Additional Journal Information:
Journal Volume: 10; Journal Issue: na; Journal ID: ISSN 1664-302X
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; photoheterotrophy; proteorhodopsin; aerobic anoxygenic phototrophy; microbial loop; free-living bacterioplankton; particle-attached bacterioplankton

Citation Formats

Gómez-Consarnau, Laura, Needham, David M., Weber, Peter K., Fuhrman, Jed A., and Mayali, Xavier. Influence of Light on Particulate Organic Matter Utilization by Attached and Free-Living Marine Bacteria. United States: N. p., 2019. Web. doi:10.3389/fmicb.2019.01204.
Gómez-Consarnau, Laura, Needham, David M., Weber, Peter K., Fuhrman, Jed A., & Mayali, Xavier. Influence of Light on Particulate Organic Matter Utilization by Attached and Free-Living Marine Bacteria. United States. doi:10.3389/fmicb.2019.01204.
Gómez-Consarnau, Laura, Needham, David M., Weber, Peter K., Fuhrman, Jed A., and Mayali, Xavier. Tue . "Influence of Light on Particulate Organic Matter Utilization by Attached and Free-Living Marine Bacteria". United States. doi:10.3389/fmicb.2019.01204. https://www.osti.gov/servlets/purl/1548317.
@article{osti_1548317,
title = {Influence of Light on Particulate Organic Matter Utilization by Attached and Free-Living Marine Bacteria},
author = {Gómez-Consarnau, Laura and Needham, David M. and Weber, Peter K. and Fuhrman, Jed A. and Mayali, Xavier},
abstractNote = {Light takes on a central role on primary productivity of aquatic systems. However, its potential impact on the degradation of photosynthetically produced biomass is not well understood. We investigated the patterns of light-induced particle breakdown and bacterial assimilation of detrital C and N using 13C and 15N labeled freeze-thawed diatom cells incubated in laboratory microcosms with a marine microbial community freshly collected from the Pacific Ocean. Particles incubated in the dark resulted in increased bacterial counts and dissolved organic carbon concentrations compared to those incubated in the light. Light also influenced the attached and free-living microbial community structure as detected by 16S rRNA gene amplicon sequencing. For example, Sphingobacteriia were enriched on dark-incubated particles and taxa from the family Flavobacteriaceae and the genus Pseudoalteromonas were numerically enriched on particles in the light. Isotope incorporation analysis by phylogenetic microarray and NanoSIMS (a method called Chip-SIP) identified free-living and attached microbial taxa able to incorporate N and C from the particles. Some taxa, including members of the Flavobacteriaceae and Cryomorphaceae, exhibited increased isotope incorporation in the light, suggesting the use of photoheterotrophic metabolisms. Yet, some members of Oceanospirillales and Rhodospirillales showed decreased isotope incorporation in the light, suggesting that their heterotrophic metabolism, particularly when occurring on particles, might increase at night or may be inhibited by sunlight. These results show that light influences particle degradation and C and N incorporation by attached bacteria, suggesting that the transfer between particulate and free-living phases are likely affected by external factors that change with the light regime, such as time of day, water column depth and season.},
doi = {10.3389/fmicb.2019.01204},
journal = {Frontiers in Microbiology},
number = na,
volume = 10,
place = {United States},
year = {2019},
month = {6}
}

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