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Title: The Mars1 kinase confers photoprotection through signaling in the chloroplast unfolded protein response

Abstract

In response to proteotoxic stress, chloroplasts communicate with the nuclear gene expression system through a chloroplast unfolded protein response (cpUPR). We isolated Chlamydomonas reinhardtii mutants that disrupt cpUPR signaling and identified a gene encoding a previously uncharacterized cytoplasmic protein kinase, termed Mars1—for mutant affected in chloroplast-to-nucleus retrograde signaling—as the first known component in cpUPR signal transmission. Lack of cpUPR induction in MARS1 mutant cells impaired their ability to cope with chloroplast stress, including exposure to excessive light. Conversely, transgenic activation of cpUPR signaling conferred an advantage to cells undergoing photooxidative stress. Our results indicate that the cpUPR mitigates chloroplast photodamage and that manipulation of this pathway is a potential avenue for engineering photosynthetic organisms with increased tolerance to chloroplast stress.

Authors:
ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1];  [1]; ORCiD logo [2]; ORCiD logo [3]; ORCiD logo [1]; ORCiD logo [1]
  1. Univ. of California, San Francisco, CA (United States). Howard Hughes Medical Inst., and Dept. of Biochemistry and Biophysics
  2. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Molecular Biophysics and Integrated Bioimaging Division
  3. Princeton Univ. NJ (United States). Dept. of Molecular Biology
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1572856
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
eLife
Additional Journal Information:
Journal Volume: 8; Journal ID: ISSN 2050-084X
Publisher:
eLife Sciences Publications, Ltd.
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Perlaza, Karina, Toutkoushian, Hannah, Boone, Morgane, Lam, Mable, Iwai, Masakazu, Jonikas, Martin C., Walter, Peter, and Ramundo, Silvia. The Mars1 kinase confers photoprotection through signaling in the chloroplast unfolded protein response. United States: N. p., 2019. Web. doi:10.7554/eLife.49577.
Perlaza, Karina, Toutkoushian, Hannah, Boone, Morgane, Lam, Mable, Iwai, Masakazu, Jonikas, Martin C., Walter, Peter, & Ramundo, Silvia. The Mars1 kinase confers photoprotection through signaling in the chloroplast unfolded protein response. United States. doi:10.7554/eLife.49577.
Perlaza, Karina, Toutkoushian, Hannah, Boone, Morgane, Lam, Mable, Iwai, Masakazu, Jonikas, Martin C., Walter, Peter, and Ramundo, Silvia. Tue . "The Mars1 kinase confers photoprotection through signaling in the chloroplast unfolded protein response". United States. doi:10.7554/eLife.49577. https://www.osti.gov/servlets/purl/1572856.
@article{osti_1572856,
title = {The Mars1 kinase confers photoprotection through signaling in the chloroplast unfolded protein response},
author = {Perlaza, Karina and Toutkoushian, Hannah and Boone, Morgane and Lam, Mable and Iwai, Masakazu and Jonikas, Martin C. and Walter, Peter and Ramundo, Silvia},
abstractNote = {In response to proteotoxic stress, chloroplasts communicate with the nuclear gene expression system through a chloroplast unfolded protein response (cpUPR). We isolated Chlamydomonas reinhardtii mutants that disrupt cpUPR signaling and identified a gene encoding a previously uncharacterized cytoplasmic protein kinase, termed Mars1—for mutant affected in chloroplast-to-nucleus retrograde signaling—as the first known component in cpUPR signal transmission. Lack of cpUPR induction in MARS1 mutant cells impaired their ability to cope with chloroplast stress, including exposure to excessive light. Conversely, transgenic activation of cpUPR signaling conferred an advantage to cells undergoing photooxidative stress. Our results indicate that the cpUPR mitigates chloroplast photodamage and that manipulation of this pathway is a potential avenue for engineering photosynthetic organisms with increased tolerance to chloroplast stress.},
doi = {10.7554/eLife.49577},
journal = {eLife},
number = ,
volume = 8,
place = {United States},
year = {2019},
month = {10}
}

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