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Title: Coexpressed subunits of dual genetic origin define a conserved supercomplex mediating essential protein import into chloroplasts

Journal Article · · Proceedings of the National Academy of Sciences of the United States of America
ORCiD logo [1];  [2]; ORCiD logo [3];  [3]; ORCiD logo [1];  [4];  [5];  [6];  [6];  [6];  [7]; ORCiD logo [8]; ORCiD logo [9]; ORCiD logo [10];  [3]; ORCiD logo [2]; ORCiD logo [6]; ORCiD logo [1]
  1. Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143,, Howard Hughes Medical Institute, Chevy Chase, MD 20815,
  2. Laboratory of Organelle Biology, Institute for Protein Research, Osaka University, Osaka 565-0871, Japan,
  3. Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095,
  4. Department of Biology, University of York, York YO10 5DD, United Kingdom,
  5. Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan,
  6. Department of Molecular Biology, University of Geneva, Geneva CH-1211, Switzerland,, Department of Plant Biology, University of Geneva, Geneva CH-1211, Switzerland,
  7. Institute of Plant Biology and Biotechnology, University of Münster, Münster 48143, Germany,
  8. Department of Biochemistry, University of Geneva, Geneva CH-1211, Switzerland,
  9. Institute of Plant Biology and Biotechnology, University of Münster, Münster 48143, Germany,, Institute of Plant Science and Resources, Okayama University, Kurashiki 710-0046, Japan,
  10. Howard Hughes Medical Institute, Chevy Chase, MD 20815,, Department of Molecular Biology, Princeton University, Princeton, NJ 08540
+ Show Author Affiliations

Significance Chloroplasts are of vital importance in photosynthetic eukaryotic organisms. Like mitochondria, they contain their own genomes. Nevertheless, most chloroplast proteins are encoded by nuclear genes, translated in the cytosol, and must cross the chloroplast envelope membranes to reach their proper destinations inside the organelle. Despite its fundamental role, our knowledge of the machinery catalyzing chloroplast protein import is incomplete and controversial. Here, we address the evolutionary conservation, composition, and function of the chloroplast protein import machinery using the green alga Chlamydomonas reinhardtii as a model system. Our findings help clarify the current debate regarding the composition of the chloroplast protein import machinery, provide evidence for cross-compartmental coordination of its biogenesis, and open promising avenues for its structural characterization.

Sponsoring Organization:
USDOE
Grant/Contract Number:
FC02-02ER63421
OSTI ID:
1730999
Alternate ID(s):
OSTI ID: 1816800
Journal Information:
Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Issue: 51 Vol. 117; ISSN 0027-8424
Publisher:
Proceedings of the National Academy of SciencesCopyright Statement
Country of Publication:
United States
Language:
English

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