Chlamydomonas mutants lacking chloroplast TRIOSE PHOSPHATE TRANSPORTER3 are metabolically compromised and light sensitive

Huang, (黄伟超), Weichao; Krishnan, Anagha; Plett, Anastasija; Meagher, Michelle; Linka, Nicole; Wang, Yongsheng; Ren, Bijie; Findinier, Justin; Redekop, Petra; Fakhimi, Neda; Kim, Rick G.; Karns, Devin A.; Boyle, Nanette; Posewitz, Matthew C.; Grossman, Arthur R.

doi:10.1093/plcell/koad095

Title: Chlamydomonas mutants lacking chloroplast TRIOSE PHOSPHATE TRANSPORTER3 are metabolically compromised and light sensitive

Journal Article · 26 March 2023 · The Plant Cell

DOI: https://doi.org/10.1093/plcell/koad095 · OSTI ID:1986579

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Abstract Modulation of photoassimilate export from the chloroplast is essential for controlling the distribution of fixed carbon in the cell and maintaining optimum photosynthetic rates. In this study, we identified chloroplast TRIOSE PHOSPHATE/PHOSPHATE TRANSLOCATOR 2 (CreTPT2) and CreTPT3 in the green alga Chlamydomonas (Chlamydomonas reinhardtii), which exhibit similar substrate specificities but whose encoding genes are differentially expressed over the diurnal cycle. We focused mostly on CreTPT3 because of its high level of expression and the severe phenotype exhibited by tpt3 relative to tpt2 mutants. Null mutants for CreTPT3 had a pleiotropic phenotype that affected growth, photosynthetic activities, metabolite profiles, carbon partitioning, and organelle-specific accumulation of H2O2. These analyses demonstrated that CreTPT3 is a dominant conduit on the chloroplast envelope for the transport of photoassimilates. In addition, CreTPT3 can serve as a safety valve that moves excess reductant out of the chloroplast and appears to be essential for preventing cells from experiencing oxidative stress and accumulating reactive oxygen species, even under low/moderate light intensities. Finally, our studies indicate subfunctionalization of the TRIOSE PHOSPHATE/PHOSPHATE TRANSLOCATOR (CreTPT) transporters and suggest that there are differences in managing the export of photoassimilates from the chloroplasts of Chlamydomonas and vascular plants.

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Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0018301; SC0019341; SC0019417

OSTI ID:: 1986579

Journal Information:: The Plant Cell, Journal Name: The Plant Cell Journal Issue: 7 Vol. 35; ISSN 1040-4651

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

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