Integration of renewable deep eutectic solvents with engineered biomass to achieve a closed-loop biorefinery
Abstract
Despite the enormous potential shown by recent biorefineries, the current bioeconomy still encounters multifaceted challenges. To develop a sustainable biorefinery in the future, multidisciplinary research will be essential to tackle technical difficulties. Herein, we leveraged a known plant genetic engineering approach that results in aldehyde-rich lignin via down-regulation of cinnamyl alcohol dehydrogenase ( CAD ) and disruption of monolignol biosynthesis. We also report on renewable deep eutectic solvents (DESs) synthesized from phenolic aldehydes that can be obtained from CAD mutant biomass. The transgenic Arabidopsis thaliana CAD mutant was pretreated with the DESs and showed a twofold increase in the yield of fermentable sugars compared with wild type (WT) upon enzymatic saccharification. Integrated use of low-recalcitrance engineered biomass, characterized by its aldehyde-type lignin subunits, in combination with a DES-based pretreatment, was found to be an effective approach for producing a high yield of sugars typically used for cellulosic biofuels and biobased chemicals. This study demonstrates that integration of renewable DES with plant genetic engineering is a promising strategy in developing a closed-loop process.
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1529169
- Grant/Contract Number:
- AC05-00OR22725; AC02-05CH11231
- Resource Type:
- Published Article
- Journal Name:
- Proceedings of the National Academy of Sciences of the United States of America
- Additional Journal Information:
- Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Volume: 116 Journal Issue: 28; Journal ID: ISSN 0027-8424
- Publisher:
- Proceedings of the National Academy of Sciences
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Kim, Kwang Ho, Eudes, Aymerick, Jeong, Keunhong, Yoo, Chang Geun, Kim, Chang Soo, and Ragauskas, Arthur. Integration of renewable deep eutectic solvents with engineered biomass to achieve a closed-loop biorefinery. United States: N. p., 2019.
Web. doi:10.1073/pnas.1904636116.
Kim, Kwang Ho, Eudes, Aymerick, Jeong, Keunhong, Yoo, Chang Geun, Kim, Chang Soo, & Ragauskas, Arthur. Integration of renewable deep eutectic solvents with engineered biomass to achieve a closed-loop biorefinery. United States. doi:10.1073/pnas.1904636116.
Kim, Kwang Ho, Eudes, Aymerick, Jeong, Keunhong, Yoo, Chang Geun, Kim, Chang Soo, and Ragauskas, Arthur. Mon .
"Integration of renewable deep eutectic solvents with engineered biomass to achieve a closed-loop biorefinery". United States. doi:10.1073/pnas.1904636116.
@article{osti_1529169,
title = {Integration of renewable deep eutectic solvents with engineered biomass to achieve a closed-loop biorefinery},
author = {Kim, Kwang Ho and Eudes, Aymerick and Jeong, Keunhong and Yoo, Chang Geun and Kim, Chang Soo and Ragauskas, Arthur},
abstractNote = {Despite the enormous potential shown by recent biorefineries, the current bioeconomy still encounters multifaceted challenges. To develop a sustainable biorefinery in the future, multidisciplinary research will be essential to tackle technical difficulties. Herein, we leveraged a known plant genetic engineering approach that results in aldehyde-rich lignin via down-regulation of cinnamyl alcohol dehydrogenase ( CAD ) and disruption of monolignol biosynthesis. We also report on renewable deep eutectic solvents (DESs) synthesized from phenolic aldehydes that can be obtained from CAD mutant biomass. The transgenic Arabidopsis thaliana CAD mutant was pretreated with the DESs and showed a twofold increase in the yield of fermentable sugars compared with wild type (WT) upon enzymatic saccharification. Integrated use of low-recalcitrance engineered biomass, characterized by its aldehyde-type lignin subunits, in combination with a DES-based pretreatment, was found to be an effective approach for producing a high yield of sugars typically used for cellulosic biofuels and biobased chemicals. This study demonstrates that integration of renewable DES with plant genetic engineering is a promising strategy in developing a closed-loop process.},
doi = {10.1073/pnas.1904636116},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
number = 28,
volume = 116,
place = {United States},
year = {2019},
month = {6}
}
DOI: 10.1073/pnas.1904636116
Search WorldCat to find libraries that may hold this journalWorks referenced in this record:
Lignin modification improves fermentable sugar yields for biofuel production
journal, June 2007
- Chen, Fang; Dixon, Richard A.
- Nature Biotechnology, Vol. 25, Issue 7, p. 759-761
Downregulation of Cinnamyl Alcohol Dehydrogenase (CAD) Leads to Improved Saccharification Efficiency in Switchgrass
journal, January 2011
- Fu, Chunxiang; Xiao, Xirong; Xi, Yajun
- BioEnergy Research, Vol. 4, Issue 3, p. 153-164
Lignin Valorization: Improving Lignin Processing in the Biorefinery
journal, May 2014
- Ragauskas, A. J.; Beckham, G. T.; Biddy, M. J.
- Science, Vol. 344, Issue 6185, p. 1246843-1246843
Monolignol Ferulate Transferase Introduces Chemically Labile Linkages into the Lignin Backbone
journal, April 2014
- Wilkerson, C. G.; Mansfield, S. D.; Lu, F.
- Science, Vol. 344, Issue 6179, p. 90-93
Enhancing alfalfa conversion efficiencies for sugar recovery and ethanol production by altering lignin composition
journal, June 2011
- Dien, Bruce S.; Miller, David J.; Hector, Ronald E.
- Bioresource Technology, Vol. 102, Issue 11, p. 6479-6486