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Title: Integration of renewable deep eutectic solvents with engineered biomass to achieve a closed-loop biorefinery

Abstract

Despite the enormous potential shown by recent biorefineries, the current bioeconomy still encounters multifaceted challenges. To develop a sustainable biorefinery in the future, multidisciplinary research will be essential to tackle technical difficulties. Herein, we leveraged a known plant genetic engineering approach that results in aldehyde-rich lignin via down-regulation of cinnamyl alcohol dehydrogenase ( CAD ) and disruption of monolignol biosynthesis. We also report on renewable deep eutectic solvents (DESs) synthesized from phenolic aldehydes that can be obtained from CAD mutant biomass. The transgenic Arabidopsis thaliana CAD mutant was pretreated with the DESs and showed a twofold increase in the yield of fermentable sugars compared with wild type (WT) upon enzymatic saccharification. Integrated use of low-recalcitrance engineered biomass, characterized by its aldehyde-type lignin subunits, in combination with a DES-based pretreatment, was found to be an effective approach for producing a high yield of sugars typically used for cellulosic biofuels and biobased chemicals. This study demonstrates that integration of renewable DES with plant genetic engineering is a promising strategy in developing a closed-loop process.

Authors:
ORCiD logo; ; ; ; ; ORCiD logo
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1529169
Alternate Identifier(s):
OSTI ID: 1545201; OSTI ID: 1561928
Grant/Contract Number:  
AC05-00OR22725; AC02-05CH11231
Resource Type:
Published Article
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America
Additional Journal Information:
Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Volume: 116 Journal Issue: 28; Journal ID: ISSN 0027-8424
Publisher:
Proceedings of the National Academy of Sciences
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; green solvent; bioenergy; cinnamyl alcohol dehydrogenase; lignocellulosic biomass

Citation Formats

Kim, Kwang Ho, Eudes, Aymerick, Jeong, Keunhong, Yoo, Chang Geun, Kim, Chang Soo, and Ragauskas, Arthur. Integration of renewable deep eutectic solvents with engineered biomass to achieve a closed-loop biorefinery. United States: N. p., 2019. Web. doi:10.1073/pnas.1904636116.
Kim, Kwang Ho, Eudes, Aymerick, Jeong, Keunhong, Yoo, Chang Geun, Kim, Chang Soo, & Ragauskas, Arthur. Integration of renewable deep eutectic solvents with engineered biomass to achieve a closed-loop biorefinery. United States. doi:10.1073/pnas.1904636116.
Kim, Kwang Ho, Eudes, Aymerick, Jeong, Keunhong, Yoo, Chang Geun, Kim, Chang Soo, and Ragauskas, Arthur. Mon . "Integration of renewable deep eutectic solvents with engineered biomass to achieve a closed-loop biorefinery". United States. doi:10.1073/pnas.1904636116.
@article{osti_1529169,
title = {Integration of renewable deep eutectic solvents with engineered biomass to achieve a closed-loop biorefinery},
author = {Kim, Kwang Ho and Eudes, Aymerick and Jeong, Keunhong and Yoo, Chang Geun and Kim, Chang Soo and Ragauskas, Arthur},
abstractNote = {Despite the enormous potential shown by recent biorefineries, the current bioeconomy still encounters multifaceted challenges. To develop a sustainable biorefinery in the future, multidisciplinary research will be essential to tackle technical difficulties. Herein, we leveraged a known plant genetic engineering approach that results in aldehyde-rich lignin via down-regulation of cinnamyl alcohol dehydrogenase ( CAD ) and disruption of monolignol biosynthesis. We also report on renewable deep eutectic solvents (DESs) synthesized from phenolic aldehydes that can be obtained from CAD mutant biomass. The transgenic Arabidopsis thaliana CAD mutant was pretreated with the DESs and showed a twofold increase in the yield of fermentable sugars compared with wild type (WT) upon enzymatic saccharification. Integrated use of low-recalcitrance engineered biomass, characterized by its aldehyde-type lignin subunits, in combination with a DES-based pretreatment, was found to be an effective approach for producing a high yield of sugars typically used for cellulosic biofuels and biobased chemicals. This study demonstrates that integration of renewable DES with plant genetic engineering is a promising strategy in developing a closed-loop process.},
doi = {10.1073/pnas.1904636116},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
number = 28,
volume = 116,
place = {United States},
year = {2019},
month = {6}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1073/pnas.1904636116

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Cited by: 10 works
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