skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Deep Eutectic Solvent Pretreatment of Transgenic Biomass With Increased C6C1 Lignin Monomers

Abstract

The complex and heterogeneous polyphenolic structure of lignin confers recalcitrance to plant cell walls and challenges biomass processing for agroindustrial applications. Recently, significant efforts have been made to alter lignin composition to overcome its inherent intractability. In this work, to overcome technical difficulties related to biomass recalcitrance, we report an integrated strategy combining biomass genetic engineering with a pretreatment using a bio-derived deep eutectic solvent (DES). In particular, we employed biomass from an Arabidopsis line that expressed a bacterial hydroxycinnamoyl-CoA hydratase-lyase (HCHL) in lignifying tissues, which results in the accumulation of unusual C6C1 lignin monomers and a slight decrease in lignin molecular weight. The transgenic biomass was pretreated with renewable DES that can be synthesized from lignin-derived phenols. Biomass from the HCHL plant line containing C6C1 monomers showed increased pretreatment efficiency and released more fermentable sugars up to 34% compared to WT biomass. The enhanced biomass saccharification of the HCHL line is likely due to a reduction of lignin recalcitrance caused by the overproduction of C6C1 aromatics that act as degree of polymerization (DP) reducers and higher chemical reactivity of lignin structures with such C6C1 aromatics. Overall, our findings demonstrate that strategic plant genetic engineering, along with renewable DES pretreatment,more » could enable the development of sustainable biorefinery.« less

Authors:
; ; ; ; ; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC); National Institutes of Health (NIH)
OSTI Identifier:
1595852
Alternate Identifier(s):
OSTI ID: 1604664
Grant/Contract Number:  
AC02-05CH11231; 1S10OD012254
Resource Type:
Published Article
Journal Name:
Frontiers in Plant Science
Additional Journal Information:
Journal Name: Frontiers in Plant Science Journal Volume: 10; Journal ID: ISSN 1664-462X
Publisher:
Frontiers Research Foundation
Country of Publication:
Switzerland
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; lignocellulosic biomass; lignin engineering; hydroxycinnamoyl-CoA hydrates-lyase; bioenergy; saccharification

Citation Formats

Kim, Kwang Ho, Wang, Yunxuan, Takada, Masatsugu, Eudes, Aymerick, Yoo, Chang Geun, Kim, Chang Soo, and Saddler, Jack. Deep Eutectic Solvent Pretreatment of Transgenic Biomass With Increased C6C1 Lignin Monomers. Switzerland: N. p., 2020. Web. doi:10.3389/fpls.2019.01774.
Kim, Kwang Ho, Wang, Yunxuan, Takada, Masatsugu, Eudes, Aymerick, Yoo, Chang Geun, Kim, Chang Soo, & Saddler, Jack. Deep Eutectic Solvent Pretreatment of Transgenic Biomass With Increased C6C1 Lignin Monomers. Switzerland. doi:10.3389/fpls.2019.01774.
Kim, Kwang Ho, Wang, Yunxuan, Takada, Masatsugu, Eudes, Aymerick, Yoo, Chang Geun, Kim, Chang Soo, and Saddler, Jack. Wed . "Deep Eutectic Solvent Pretreatment of Transgenic Biomass With Increased C6C1 Lignin Monomers". Switzerland. doi:10.3389/fpls.2019.01774.
@article{osti_1595852,
title = {Deep Eutectic Solvent Pretreatment of Transgenic Biomass With Increased C6C1 Lignin Monomers},
author = {Kim, Kwang Ho and Wang, Yunxuan and Takada, Masatsugu and Eudes, Aymerick and Yoo, Chang Geun and Kim, Chang Soo and Saddler, Jack},
abstractNote = {The complex and heterogeneous polyphenolic structure of lignin confers recalcitrance to plant cell walls and challenges biomass processing for agroindustrial applications. Recently, significant efforts have been made to alter lignin composition to overcome its inherent intractability. In this work, to overcome technical difficulties related to biomass recalcitrance, we report an integrated strategy combining biomass genetic engineering with a pretreatment using a bio-derived deep eutectic solvent (DES). In particular, we employed biomass from an Arabidopsis line that expressed a bacterial hydroxycinnamoyl-CoA hydratase-lyase (HCHL) in lignifying tissues, which results in the accumulation of unusual C6C1 lignin monomers and a slight decrease in lignin molecular weight. The transgenic biomass was pretreated with renewable DES that can be synthesized from lignin-derived phenols. Biomass from the HCHL plant line containing C6C1 monomers showed increased pretreatment efficiency and released more fermentable sugars up to 34% compared to WT biomass. The enhanced biomass saccharification of the HCHL line is likely due to a reduction of lignin recalcitrance caused by the overproduction of C6C1 aromatics that act as degree of polymerization (DP) reducers and higher chemical reactivity of lignin structures with such C6C1 aromatics. Overall, our findings demonstrate that strategic plant genetic engineering, along with renewable DES pretreatment, could enable the development of sustainable biorefinery.},
doi = {10.3389/fpls.2019.01774},
journal = {Frontiers in Plant Science},
number = ,
volume = 10,
place = {Switzerland},
year = {2020},
month = {1}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.3389/fpls.2019.01774

Citation Metrics:
Cited by: 1 work
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

Expression of a bacterial 3-dehydroshikimate dehydratase reduces lignin content and improves biomass saccharification efficiency
journal, January 2015

  • Eudes, Aymerick; Sathitsuksanoh, Noppadon; Baidoo, Edward E. K.
  • Plant Biotechnology Journal, Vol. 13, Issue 9
  • DOI: 10.1111/pbi.12310

Lignin Valorization: Improving Lignin Processing in the Biorefinery
journal, May 2014

  • Ragauskas, A. J.; Beckham, G. T.; Biddy, M. J.
  • Science, Vol. 344, Issue 6185, p. 1246843-1246843
  • DOI: 10.1126/science.1246843

Natural genetic variability reduces recalcitrance in poplar
journal, May 2016

  • Bhagia, Samarthya; Muchero, Wellington; Kumar, Rajeev
  • Biotechnology for Biofuels, Vol. 9, Issue 1
  • DOI: 10.1186/s13068-016-0521-2

Opportunities, recent trends and challenges of integrated biorefinery: Part I
journal, March 2015


Solution-state 2D NMR of ball-milled plant cell wall gels in DMSO-d6/pyridine-d5
journal, January 2010

  • Kim, Hoon; Ralph, John
  • Org. Biomol. Chem., Vol. 8, Issue 3
  • DOI: 10.1039/b916070a

Integration of renewable deep eutectic solvents with engineered biomass to achieve a closed-loop biorefinery
journal, June 2019

  • Kim, Kwang Ho; Eudes, Aymerick; Jeong, Keunhong
  • Proceedings of the National Academy of Sciences, Vol. 116, Issue 28
  • DOI: 10.1073/pnas.1904636116

Lignin structure and its engineering
journal, April 2019


Lignin modification improves fermentable sugar yields for biofuel production
journal, June 2007

  • Chen, Fang; Dixon, Richard A.
  • Nature Biotechnology, Vol. 25, Issue 7, p. 759-761
  • DOI: 10.1038/nbt1316

Computational Study of Bond Dissociation Enthalpies for a Large Range of Native and Modified Lignins
journal, October 2011

  • Kim, Seonah; Chmely, Stephen C.; Nimlos, Mark R.
  • The Journal of Physical Chemistry Letters, Vol. 2, Issue 22
  • DOI: 10.1021/jz201182w

Surface and ultrastructural characterization of raw and pretreated switchgrass
journal, December 2011


Ionic liquid solvent properties as predictors of lignocellulose pretreatment efficacy
journal, January 2010

  • Doherty, Thomas V.; Mora-Pale, Mauricio; Foley, Sage E.
  • Green Chemistry, Vol. 12, Issue 11
  • DOI: 10.1039/c0gc00206b

How Does Plant Cell Wall Nanoscale Architecture Correlate with Enzymatic Digestibility?
journal, November 2012


Natural deep eutectic solvents as new potential media for green technology
journal, March 2013


Efficient biomass pretreatment using ionic liquids derived from lignin and hemicellulose
journal, August 2014

  • Socha, A. M.; Parthasarathi, R.; Shi, J.
  • Proceedings of the National Academy of Sciences, Vol. 111, Issue 35
  • DOI: 10.1073/pnas.1405685111

Silencing of 4-coumarate:coenzyme A ligase in switchgrass leads to reduced lignin content and improved fermentable sugar yields for biofuel production
journal, July 2011


Biomass resource facilities and biomass conversion processing for fuels and chemicals
journal, July 2001


Biomass pretreatment: Fundamentals toward application
journal, November 2011


Impact of lignin polymer backbone esters on ionic liquid pretreatment of poplar
journal, April 2017


Comparative evaluation of Populus variants total sugar release and structural features following pretreatment and digestion by two distinct biological systems
journal, November 2017

  • Thomas, Vanessa A.; Kothari, Ninad; Bhagia, Samarthya
  • Biotechnology for Biofuels, Vol. 10, Issue 1
  • DOI: 10.1186/s13068-017-0975-x

Take a Closer Look: Biofuels Can Support Environmental, Economic and Social Goals
journal, June 2014

  • Dale, Bruce E.; Anderson, James E.; Brown, Robert C.
  • Environmental Science & Technology, Vol. 48, Issue 13
  • DOI: 10.1021/es5025433

Can genetic engineering of lignin deposition be accomplished without an unacceptable yield penalty?
journal, April 2013


Monolignol Ferulate Transferase Introduces Chemically Labile Linkages into the Lignin Backbone
journal, April 2014


Evolution and current status of research in phenolic compounds
journal, November 2007


Disruption of Mediator rescues the stunted growth of a lignin-deficient Arabidopsis mutant
journal, March 2014

  • Bonawitz, Nicholas D.; Kim, Jeong Im; Tobimatsu, Yuki
  • Nature, Vol. 509, Issue 7500, p. 376-380
  • DOI: 10.1038/nature13084

Mutations in the cinnamate 4-hydroxylase gene impact metabolism, growth and development in Arabidopsis: Arabidopsis C4H mutants
journal, December 2009


Contribution of Deep Eutectic Solvents for Biomass Processing: Opportunities, Challenges, and Limitations
journal, March 2015

  • Vigier, Karine De Oliveira; Chatel, Gregory; Jérôme, François
  • ChemCatChem, Vol. 7, Issue 8
  • DOI: 10.1002/cctc.201500134

Comparative sugar recovery data from laboratory scale application of leading pretreatment technologies to corn stover
journal, December 2005