Deep Eutectic Solvent Pretreatment of Transgenic Biomass With Increased C6C1 Lignin Monomers
Abstract
The complex and heterogeneous polyphenolic structure of lignin confers recalcitrance to plant cell walls and challenges biomass processing for agroindustrial applications. Recently, significant efforts have been made to alter lignin composition to overcome its inherent intractability. In this work, to overcome technical difficulties related to biomass recalcitrance, we report an integrated strategy combining biomass genetic engineering with a pretreatment using a bio-derived deep eutectic solvent (DES). In particular, we employed biomass from an Arabidopsis line that expressed a bacterial hydroxycinnamoyl-CoA hydratase-lyase (HCHL) in lignifying tissues, which results in the accumulation of unusual C6C1 lignin monomers and a slight decrease in lignin molecular weight. The transgenic biomass was pretreated with renewable DES that can be synthesized from lignin-derived phenols. Biomass from the HCHL plant line containing C6C1 monomers showed increased pretreatment efficiency and released more fermentable sugars up to 34% compared to WT biomass. The enhanced biomass saccharification of the HCHL line is likely due to a reduction of lignin recalcitrance caused by the overproduction of C6C1 aromatics that act as degree of polymerization (DP) reducers and higher chemical reactivity of lignin structures with such C6C1 aromatics. Overall, our findings demonstrate that strategic plant genetic engineering, along with renewable DES pretreatment,more »
- Authors:
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC); National Institutes of Health (NIH)
- OSTI Identifier:
- 1595852
- Alternate Identifier(s):
- OSTI ID: 1604664
- Grant/Contract Number:
- AC02-05CH11231; 1S10OD012254
- Resource Type:
- Published Article
- Journal Name:
- Frontiers in Plant Science
- Additional Journal Information:
- Journal Name: Frontiers in Plant Science Journal Volume: 10; Journal ID: ISSN 1664-462X
- Publisher:
- Frontiers Research Foundation
- Country of Publication:
- Switzerland
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; lignocellulosic biomass; lignin engineering; hydroxycinnamoyl-CoA hydrates-lyase; bioenergy; saccharification
Citation Formats
Kim, Kwang Ho, Wang, Yunxuan, Takada, Masatsugu, Eudes, Aymerick, Yoo, Chang Geun, Kim, Chang Soo, and Saddler, Jack. Deep Eutectic Solvent Pretreatment of Transgenic Biomass With Increased C6C1 Lignin Monomers. Switzerland: N. p., 2020.
Web. doi:10.3389/fpls.2019.01774.
Kim, Kwang Ho, Wang, Yunxuan, Takada, Masatsugu, Eudes, Aymerick, Yoo, Chang Geun, Kim, Chang Soo, & Saddler, Jack. Deep Eutectic Solvent Pretreatment of Transgenic Biomass With Increased C6C1 Lignin Monomers. Switzerland. doi:10.3389/fpls.2019.01774.
Kim, Kwang Ho, Wang, Yunxuan, Takada, Masatsugu, Eudes, Aymerick, Yoo, Chang Geun, Kim, Chang Soo, and Saddler, Jack. Wed .
"Deep Eutectic Solvent Pretreatment of Transgenic Biomass With Increased C6C1 Lignin Monomers". Switzerland. doi:10.3389/fpls.2019.01774.
@article{osti_1595852,
title = {Deep Eutectic Solvent Pretreatment of Transgenic Biomass With Increased C6C1 Lignin Monomers},
author = {Kim, Kwang Ho and Wang, Yunxuan and Takada, Masatsugu and Eudes, Aymerick and Yoo, Chang Geun and Kim, Chang Soo and Saddler, Jack},
abstractNote = {The complex and heterogeneous polyphenolic structure of lignin confers recalcitrance to plant cell walls and challenges biomass processing for agroindustrial applications. Recently, significant efforts have been made to alter lignin composition to overcome its inherent intractability. In this work, to overcome technical difficulties related to biomass recalcitrance, we report an integrated strategy combining biomass genetic engineering with a pretreatment using a bio-derived deep eutectic solvent (DES). In particular, we employed biomass from an Arabidopsis line that expressed a bacterial hydroxycinnamoyl-CoA hydratase-lyase (HCHL) in lignifying tissues, which results in the accumulation of unusual C6C1 lignin monomers and a slight decrease in lignin molecular weight. The transgenic biomass was pretreated with renewable DES that can be synthesized from lignin-derived phenols. Biomass from the HCHL plant line containing C6C1 monomers showed increased pretreatment efficiency and released more fermentable sugars up to 34% compared to WT biomass. The enhanced biomass saccharification of the HCHL line is likely due to a reduction of lignin recalcitrance caused by the overproduction of C6C1 aromatics that act as degree of polymerization (DP) reducers and higher chemical reactivity of lignin structures with such C6C1 aromatics. Overall, our findings demonstrate that strategic plant genetic engineering, along with renewable DES pretreatment, could enable the development of sustainable biorefinery.},
doi = {10.3389/fpls.2019.01774},
journal = {Frontiers in Plant Science},
number = ,
volume = 10,
place = {Switzerland},
year = {2020},
month = {1}
}
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.3389/fpls.2019.01774
DOI: 10.3389/fpls.2019.01774
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 1 work
Citation information provided by
Web of Science
Web of Science
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Expression of a bacterial 3-dehydroshikimate dehydratase reduces lignin content and improves biomass saccharification efficiency
journal, January 2015
- Eudes, Aymerick; Sathitsuksanoh, Noppadon; Baidoo, Edward E. K.
- Plant Biotechnology Journal, Vol. 13, Issue 9
Lignin Valorization: Improving Lignin Processing in the Biorefinery
journal, May 2014
- Ragauskas, A. J.; Beckham, G. T.; Biddy, M. J.
- Science, Vol. 344, Issue 6185, p. 1246843-1246843
Strong decrease in lignin content without significant alteration of plant development is induced by simultaneous down-regulation of cinnamoyl CoA reductase (CCR) and cinnamyl alcohol dehydrogenase (CAD) in tobacco plants: Simultaneous down-regulation of CAD and CCR
journal, November 2001
- Chabannes, Matthieu; Barakate, Abdellah; Lapierre, Catherine
- The Plant Journal, Vol. 28, Issue 3
Natural genetic variability reduces recalcitrance in poplar
journal, May 2016
- Bhagia, Samarthya; Muchero, Wellington; Kumar, Rajeev
- Biotechnology for Biofuels, Vol. 9, Issue 1
Opportunities, recent trends and challenges of integrated biorefinery: Part I
journal, March 2015
- Maity, Sunil K.
- Renewable and Sustainable Energy Reviews, Vol. 43
Solution-state 2D NMR of ball-milled plant cell wall gels in DMSO-d6/pyridine-d5
journal, January 2010
- Kim, Hoon; Ralph, John
- Org. Biomol. Chem., Vol. 8, Issue 3
Integration of renewable deep eutectic solvents with engineered biomass to achieve a closed-loop biorefinery
journal, June 2019
- Kim, Kwang Ho; Eudes, Aymerick; Jeong, Keunhong
- Proceedings of the National Academy of Sciences, Vol. 116, Issue 28
Lignin structure and its engineering
journal, April 2019
- Ralph, John; Lapierre, Catherine; Boerjan, Wout
- Current Opinion in Biotechnology, Vol. 56
Lignin modification improves fermentable sugar yields for biofuel production
journal, June 2007
- Chen, Fang; Dixon, Richard A.
- Nature Biotechnology, Vol. 25, Issue 7, p. 759-761
Computational Study of Bond Dissociation Enthalpies for a Large Range of Native and Modified Lignins
journal, October 2011
- Kim, Seonah; Chmely, Stephen C.; Nimlos, Mark R.
- The Journal of Physical Chemistry Letters, Vol. 2, Issue 22
Surface and ultrastructural characterization of raw and pretreated switchgrass
journal, December 2011
- Donohoe, Bryon S.; Vinzant, Todd B.; Elander, Richard T.
- Bioresource Technology, Vol. 102, Issue 24
Ionic liquid solvent properties as predictors of lignocellulose pretreatment efficacy
journal, January 2010
- Doherty, Thomas V.; Mora-Pale, Mauricio; Foley, Sage E.
- Green Chemistry, Vol. 12, Issue 11
How Does Plant Cell Wall Nanoscale Architecture Correlate with Enzymatic Digestibility?
journal, November 2012
- Ding, S. -Y.; Liu, Y. -S.; Zeng, Y.
- Science, Vol. 338, Issue 6110
Natural deep eutectic solvents as new potential media for green technology
journal, March 2013
- Dai, Yuntao; van Spronsen, Jaap; Witkamp, Geert-Jan
- Analytica Chimica Acta, Vol. 766
Efficient biomass pretreatment using ionic liquids derived from lignin and hemicellulose
journal, August 2014
- Socha, A. M.; Parthasarathi, R.; Shi, J.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 35
Biosynthesis and incorporation of side-chain-truncated lignin monomers to reduce lignin polymerization and enhance saccharification: Reduction in lignin DP enhances saccharification
journal, March 2012
- Eudes, Aymerick; George, Anthe; Mukerjee, Purba
- Plant Biotechnology Journal, Vol. 10, Issue 5
Silencing of 4-coumarate:coenzyme A ligase in switchgrass leads to reduced lignin content and improved fermentable sugar yields for biofuel production
journal, July 2011
- Xu, Bin; Escamilla-Treviño, Luis L.; Sathitsuksanoh, Noppadon
- New Phytologist, Vol. 192, Issue 3
Biomass resource facilities and biomass conversion processing for fuels and chemicals
journal, July 2001
- Demirbaş, Ayhan
- Energy Conversion and Management, Vol. 42, Issue 11
Biomass pretreatment: Fundamentals toward application
journal, November 2011
- Agbor, Valery B.; Cicek, Nazim; Sparling, Richard
- Biotechnology Advances, Vol. 29, Issue 6
Impact of lignin polymer backbone esters on ionic liquid pretreatment of poplar
journal, April 2017
- Kim, Kwang Ho; Dutta, Tanmoy; Ralph, John
- Biotechnology for Biofuels, Vol. 10, Issue 1
Comparative evaluation of Populus variants total sugar release and structural features following pretreatment and digestion by two distinct biological systems
journal, November 2017
- Thomas, Vanessa A.; Kothari, Ninad; Bhagia, Samarthya
- Biotechnology for Biofuels, Vol. 10, Issue 1
Take a Closer Look: Biofuels Can Support Environmental, Economic and Social Goals
journal, June 2014
- Dale, Bruce E.; Anderson, James E.; Brown, Robert C.
- Environmental Science & Technology, Vol. 48, Issue 13
Can genetic engineering of lignin deposition be accomplished without an unacceptable yield penalty?
journal, April 2013
- Bonawitz, Nicholas D.; Chapple, Clint
- Current Opinion in Biotechnology, Vol. 24, Issue 2
Monolignol Ferulate Transferase Introduces Chemically Labile Linkages into the Lignin Backbone
journal, April 2014
- Wilkerson, C. G.; Mansfield, S. D.; Lu, F.
- Science, Vol. 344, Issue 6179, p. 90-93
Evolution and current status of research in phenolic compounds
journal, November 2007
- Boudet, Alain-Michel
- Phytochemistry, Vol. 68, Issue 22-24
Disruption of Mediator rescues the stunted growth of a lignin-deficient Arabidopsis mutant
journal, March 2014
- Bonawitz, Nicholas D.; Kim, Jeong Im; Tobimatsu, Yuki
- Nature, Vol. 509, Issue 7500, p. 376-380
Recent Efforts to Prevent Undesirable Reactions From Fractionation to Depolymerization of Lignin: Toward Maximizing the Value From Lignin
journal, September 2018
- Kim, Kwang Ho; Kim, Chang Soo
- Frontiers in Energy Research, Vol. 6
Mutations in the cinnamate 4-hydroxylase gene impact metabolism, growth and development in Arabidopsis: Arabidopsis C4H mutants
journal, December 2009
- Schilmiller, Anthony L.; Stout, Jake; Weng, Jing-Ke
- The Plant Journal, Vol. 60, Issue 5
Contribution of Deep Eutectic Solvents for Biomass Processing: Opportunities, Challenges, and Limitations
journal, March 2015
- Vigier, Karine De Oliveira; Chatel, Gregory; Jérôme, François
- ChemCatChem, Vol. 7, Issue 8
4-Hydroxycinnamoyl-CoA hydratase/lyase, an enzyme of phenylpropanoid cleavage from Pseudomonas , causes formation of C 6 -C 1 acid and alcohol glucose conjugates when expressed in hairy roots of Datura stramonium L.
journal, May 2002
- Mitra, Adinpunya; Mayer, Melinda; Mellon, Fred
- Planta, Vol. 215, Issue 1
Elucidating Structural Characteristics of Biomass using Solution-State 2 D NMR with a Mixture of Deuterated Dimethylsulfoxide and Hexamethylphosphoramide
journal, April 2016
- Yoo, Chang Geun; Pu, Yunqiao; Li, Mi
- ChemSusChem, Vol. 9, Issue 10
Comparative sugar recovery data from laboratory scale application of leading pretreatment technologies to corn stover
journal, December 2005
- Wyman, Charles E.; Dale, Bruce E.; Elander, Richard T.
- Bioresource Technology, Vol. 96, Issue 18, p. 2026-2032