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Title: Inclusion of Dietary Defatted Microalgae Dose-Dependently Enriches ω-3 Fatty Acids in Egg Yolk and Tissues of Laying Hens

Abstract

ABSTRACT Background The potential for dietary microalgae to enrich eggs of laying hens with ω-3 (n–3) fatty acids, and the mechanisms involved, are unclear. Objectives The aim of this study was to determine the effects and molecular regulation of a defatted Nannochloropsis oceanica microalgae (DNOM) biomass on the enrichment of the eggs and tissues of laying hens with ω-3 fatty acids. Methods Fifty Shaver-White Leghorn hens (46 wk of age, body weight: 1.70 ± 0.27 kg) were individually caged (n = 10) and fed a corn-soy–based diet supplemented with DNOM at 0% (control), 2.86%, 5.75%, 11.5%, and 23% for 6 wk. Fatty acid profiles, health status, and related gene expression in eggs, blood, and tissues were performed at weeks 0, 2, 4, and 6. Data were analyzed by a combination of 1-factor ANOVA and correlation between DNOM doses and measures. Results The DNOM produced linear (P < 0.01) enrichments of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and total ω-3 fatty acids in the egg yolk (R2 > 0.9) and of DHA in the liver, breast, and thigh (R2 = 0.66–0.82). Concentrations of EPA + DHA in the egg yolk and these 3 tissues of hens fed 11.5% and 23% DNOM were 1.4–2.1, 0.6–1, 3.3–5.3, and 6–7 times greater (P < 0.001) than those inmore » the controls, respectively. The DNOM caused dose-dependent elevations (P < 0.01) of malic enzyme and elongases 3, 4, and 5 mRNA levels (R2 = 0.97, 0.78, 0.97, and 0.86, respectively), along with increased (P < 0.01) Δ5- and Δ6-desaturases and decreased (P < 0.01) Δ9-desaturase and acyl-coenzyme A thioesterase 4 mRNA levels in the liver. Conclusions Feeding DNOM to laying hens produced dose-dependent enrichments of DHA in their eggs, liver, and muscles by regulating key genes involved in the elongation and desaturation of polyunsaturated fatty acids. Our findings will help produce DHA-enriched eggs.« less

Authors:
 [1];  [1];  [1];  [1];  [1]
  1. Department of Animal Science, Cornell University, Ithaca, NY
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1508180
Grant/Contract Number:  
201110006-30361; EE0007091
Resource Type:
Published Article
Journal Name:
Journal of Nutrition
Additional Journal Information:
Journal Name: Journal of Nutrition Journal Volume: 149 Journal Issue: 6; Journal ID: ISSN 0022-3166
Publisher:
Oxford University Press
Country of Publication:
United States
Language:
English

Citation Formats

Manor, Meghan L., Derksen, Theodore J., Magnuson, Andrew D., Raza, Fahad, and Lei, Xin Gen. Inclusion of Dietary Defatted Microalgae Dose-Dependently Enriches ω-3 Fatty Acids in Egg Yolk and Tissues of Laying Hens. United States: N. p., 2019. Web. doi:10.1093/jn/nxz032.
Manor, Meghan L., Derksen, Theodore J., Magnuson, Andrew D., Raza, Fahad, & Lei, Xin Gen. Inclusion of Dietary Defatted Microalgae Dose-Dependently Enriches ω-3 Fatty Acids in Egg Yolk and Tissues of Laying Hens. United States. doi:10.1093/jn/nxz032.
Manor, Meghan L., Derksen, Theodore J., Magnuson, Andrew D., Raza, Fahad, and Lei, Xin Gen. Mon . "Inclusion of Dietary Defatted Microalgae Dose-Dependently Enriches ω-3 Fatty Acids in Egg Yolk and Tissues of Laying Hens". United States. doi:10.1093/jn/nxz032.
@article{osti_1508180,
title = {Inclusion of Dietary Defatted Microalgae Dose-Dependently Enriches ω-3 Fatty Acids in Egg Yolk and Tissues of Laying Hens},
author = {Manor, Meghan L. and Derksen, Theodore J. and Magnuson, Andrew D. and Raza, Fahad and Lei, Xin Gen},
abstractNote = {ABSTRACT Background The potential for dietary microalgae to enrich eggs of laying hens with ω-3 (n–3) fatty acids, and the mechanisms involved, are unclear. Objectives The aim of this study was to determine the effects and molecular regulation of a defatted Nannochloropsis oceanica microalgae (DNOM) biomass on the enrichment of the eggs and tissues of laying hens with ω-3 fatty acids. Methods Fifty Shaver-White Leghorn hens (46 wk of age, body weight: 1.70 ± 0.27 kg) were individually caged (n = 10) and fed a corn-soy–based diet supplemented with DNOM at 0% (control), 2.86%, 5.75%, 11.5%, and 23% for 6 wk. Fatty acid profiles, health status, and related gene expression in eggs, blood, and tissues were performed at weeks 0, 2, 4, and 6. Data were analyzed by a combination of 1-factor ANOVA and correlation between DNOM doses and measures. Results The DNOM produced linear (P < 0.01) enrichments of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and total ω-3 fatty acids in the egg yolk (R2 > 0.9) and of DHA in the liver, breast, and thigh (R2 = 0.66–0.82). Concentrations of EPA + DHA in the egg yolk and these 3 tissues of hens fed 11.5% and 23% DNOM were 1.4–2.1, 0.6–1, 3.3–5.3, and 6–7 times greater (P < 0.001) than those in the controls, respectively. The DNOM caused dose-dependent elevations (P < 0.01) of malic enzyme and elongases 3, 4, and 5 mRNA levels (R2 = 0.97, 0.78, 0.97, and 0.86, respectively), along with increased (P < 0.01) Δ5- and Δ6-desaturases and decreased (P < 0.01) Δ9-desaturase and acyl-coenzyme A thioesterase 4 mRNA levels in the liver. Conclusions Feeding DNOM to laying hens produced dose-dependent enrichments of DHA in their eggs, liver, and muscles by regulating key genes involved in the elongation and desaturation of polyunsaturated fatty acids. Our findings will help produce DHA-enriched eggs.},
doi = {10.1093/jn/nxz032},
journal = {Journal of Nutrition},
number = 6,
volume = 149,
place = {United States},
year = {2019},
month = {4}
}

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DOI: 10.1093/jn/nxz032

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