Improving ethanol yields with deacetylated and two-stage pretreated corn stover and sugarcane bagasse by blending commercial xylose-fermenting and wild type Saccharomyces yeast
Abstract
Corn stover and sugarcane bagasse are the most widely available agriculture processing biomass and could serve as feedstocks for production of biofuel. In this study, three different technologies are combined to develop a more efficient conversion process for each of these feedstocks. The three technologies are diluted alkaline deacetylation process, combined thermochemical and mechanical shear pretreatment, and fermentation using a combined inoculum of two commercial Saccharomyces yeast strains. The two yeast strains used were a non-GMO and GMO strain engineered for xylose fermentation. The final ethanol concentrations obtained were 35.7 g/L from deacetylated corn stover and 32.9 g/L from sugarcane bagasse. Blending the two yeast reduced residual xylose content from 1.24 g/L to 0.48g/L and increased ethanol production by 6.5% compared to solely using the C5/C6 yeast. In conclusion, the optimized yeast blend also lowered the amount of C5/C6 yeast required for inoculation by 80%.
- Authors:
-
- Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States)
- National Center for Agricultural Utilization Research, USDA, Peoria, IL (United States)
- Publication Date:
- Research Org.:
- Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1498057
- Alternate Identifier(s):
- OSTI ID: 1547670
- Grant/Contract Number:
- SC0018420
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Bioresource Technology
- Additional Journal Information:
- Journal Volume: 282; Journal Issue: C; Journal ID: ISSN 0960-8524
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; corn stover; sugarcane bagasse; deacetylation; bioethanol; mixed yeast culture; two-stage pretreatment
Citation Formats
Wang, Zhaoqin, Dien, Bruce S., Rausch, Kent D., Tumbleson, M. E., and Singh, Vijay. Improving ethanol yields with deacetylated and two-stage pretreated corn stover and sugarcane bagasse by blending commercial xylose-fermenting and wild type Saccharomyces yeast. United States: N. p., 2019.
Web. doi:10.1016/j.biortech.2019.02.123.
Wang, Zhaoqin, Dien, Bruce S., Rausch, Kent D., Tumbleson, M. E., & Singh, Vijay. Improving ethanol yields with deacetylated and two-stage pretreated corn stover and sugarcane bagasse by blending commercial xylose-fermenting and wild type Saccharomyces yeast. United States. https://doi.org/10.1016/j.biortech.2019.02.123
Wang, Zhaoqin, Dien, Bruce S., Rausch, Kent D., Tumbleson, M. E., and Singh, Vijay. Fri .
"Improving ethanol yields with deacetylated and two-stage pretreated corn stover and sugarcane bagasse by blending commercial xylose-fermenting and wild type Saccharomyces yeast". United States. https://doi.org/10.1016/j.biortech.2019.02.123. https://www.osti.gov/servlets/purl/1498057.
@article{osti_1498057,
title = {Improving ethanol yields with deacetylated and two-stage pretreated corn stover and sugarcane bagasse by blending commercial xylose-fermenting and wild type Saccharomyces yeast},
author = {Wang, Zhaoqin and Dien, Bruce S. and Rausch, Kent D. and Tumbleson, M. E. and Singh, Vijay},
abstractNote = {Corn stover and sugarcane bagasse are the most widely available agriculture processing biomass and could serve as feedstocks for production of biofuel. In this study, three different technologies are combined to develop a more efficient conversion process for each of these feedstocks. The three technologies are diluted alkaline deacetylation process, combined thermochemical and mechanical shear pretreatment, and fermentation using a combined inoculum of two commercial Saccharomyces yeast strains. The two yeast strains used were a non-GMO and GMO strain engineered for xylose fermentation. The final ethanol concentrations obtained were 35.7 g/L from deacetylated corn stover and 32.9 g/L from sugarcane bagasse. Blending the two yeast reduced residual xylose content from 1.24 g/L to 0.48g/L and increased ethanol production by 6.5% compared to solely using the C5/C6 yeast. In conclusion, the optimized yeast blend also lowered the amount of C5/C6 yeast required for inoculation by 80%.},
doi = {10.1016/j.biortech.2019.02.123},
journal = {Bioresource Technology},
number = C,
volume = 282,
place = {United States},
year = {Fri Mar 01 00:00:00 EST 2019},
month = {Fri Mar 01 00:00:00 EST 2019}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 47 works
Citation information provided by
Web of Science
Web of Science
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Evolved hexose transporter enhances xylose uptake and glucose/xylose co-utilization in Saccharomyces cerevisiae
journal, January 2016
- Reider Apel, Amanda; Ouellet, Mario; Szmidt-Middleton, Heather
- Scientific Reports, Vol. 6, Issue 1
Improved Xylan Hydrolysis of Corn Stover by Deacetylation with High Solids Dilute Acid Pretreatment
journal, December 2011
- Chen, Xiaowen; Shekiro, Joseph; Elander, Rick
- Industrial & Engineering Chemistry Research, Vol. 51, Issue 1
The impacts of deacetylation prior to dilute acid pretreatment on the bioethanol process
journal, January 2012
- Chen, Xiaowen; Shekiro, Joseph; Franden, Mary Ann
- Biotechnology for Biofuels, Vol. 5, Issue 1
Improved ethanol yield and reduced Minimum Ethanol Selling Price (MESP) by modifying low severity dilute acid pretreatment with deacetylation and mechanical refining: 1) Experimental
journal, January 2012
- Chen, Xiaowen; Tao, Ling; Shekiro, Joseph
- Biotechnology for Biofuels, Vol. 5, Issue 1
Use of tropical maize for bioethanol production
journal, March 2013
- Chen, Ming-Hsu; Kaur, Prabhjot; Dien, Bruce
- World Journal of Microbiology and Biotechnology, Vol. 29, Issue 8
DMR (deacetylation and mechanical refining) processing of corn stover achieves high monomeric sugar concentrations (230 g L −1 ) during enzymatic hydrolysis and high ethanol concentrations (>10% v/v) during fermentation without hydrolysate purification or concentration
journal, January 2016
- Chen, Xiaowen; Kuhn, Erik; Jennings, Edward W.
- Energy & Environmental Science, Vol. 9, Issue 4
Association of wet disk milling and ozonolysis as pretreatment for enzymatic saccharification of sugarcane bagasse and straw
journal, May 2013
- Barros, Rodrigo da Rocha Olivieri de; Paredes, Raquel de Sousa; Endo, Takashi
- Bioresource Technology, Vol. 136
Combination of hot compressed water treatment and wet disk milling for high sugar recovery yield in enzymatic hydrolysis of rice straw
journal, January 2012
- Hideno, Akihiro; Inoue, Hiroyuki; Yanagida, Takashi
- Bioresource Technology, Vol. 104
Biomass Recalcitrance: Engineering Plants and Enzymes for Biofuels Production
journal, February 2007
- Himmel, M. E.; Ding, S.-Y.; Johnson, D. K.
- Science, Vol. 315, Issue 5813, p. 804-807
Pretreatment and Lignocellulosic Chemistry
journal, May 2012
- Hu, Fan; Ragauskas, Art
- BioEnergy Research, Vol. 5, Issue 4
Metabolic engineering for improved fermentation of pentoses by yeasts
journal, May 2004
- Jeffries, T. W.; Jin, Y. -S.
- Applied Microbiology and Biotechnology, Vol. 63, Issue 5
Changes on structural properties of biomass pretreated by combined deacetylation with liquid hot water and its effect on enzymatic hydrolysis
journal, November 2016
- Jiang, Wei; Chang, Senlin; Qu, Yongshui
- Bioresource Technology, Vol. 220
Pretreatment of lignocellulose: Formation of inhibitory by-products and strategies for minimizing their effects
journal, January 2016
- Jönsson, Leif J.; Martín, Carlos
- Bioresource Technology, Vol. 199
Improvement of sugar yields from corn stover using sequential hot water pretreatment and disk milling
journal, September 2016
- Kim, Sun Min; Dien, Bruce S.; Tumbleson, M. E.
- Bioresource Technology, Vol. 216
Yield-determining factors in high-solids enzymatic hydrolysis of lignocellulose
journal, January 2009
- Kristensen, Jan B.; Felby, Claus; Jørgensen, Henning
- Biotechnology for Biofuels, Vol. 2, Issue 1
Value-added biotransformation of cellulosic sugars by engineered Saccharomyces cerevisiae
journal, July 2018
- Lane, Stephan; Dong, Jia; Jin, Yong-Su
- Bioresource Technology, Vol. 260
The generation of fermentation inhibitors during dilute acid hydrolysis of softwood
journal, February 1999
- Larsson, Simona; Palmqvist, Eva; Hahn-Hägerdal, Bärbel
- Enzyme and Microbial Technology, Vol. 24, Issue 3-4
Optimization of microwave-assisted calcium chloride pretreatment of corn stover
journal, January 2013
- Li, Hongqiang; Xu, Jian
- Bioresource Technology, Vol. 127
Recent advances in metabolic engineering of Saccharomyces cerevisiae: New tools and their applications
journal, November 2018
- Lian, Jiazhang; Mishra, Shekhar; Zhao, Huimin
- Metabolic Engineering, Vol. 50
Dilute acid hydrolysis of paper birch: Kinetics studies of xylan and acetyl-group hydrolysis
journal, March 1985
- Maloney, Mark T.; Chapman, Thomas W.; Baker, Andrew J.
- Biotechnology and Bioengineering, Vol. 27, Issue 3
Trends in bioconversion of lignocellulose: Biofuels, platform chemicals & biorefinery concept
journal, August 2012
- Menon, Vishnu; Rao, Mala
- Progress in Energy and Combustion Science, Vol. 38, Issue 4
Characterization of the effectiveness of hexose transporters for transporting xylose during glucose and xylose co‐fermentation by a recombinant Saccharomyces yeast
journal, June 2004
- Sedlak, Miroslav; Ho, Nancy W. Y.
- Yeast, Vol. 21, Issue 8, p. 671-684
Conversion of D-xylose into ethanol by the yeast Pachysolen tannophilus
journal, February 1981
- Schneider, H.; Wang, P. Y.; Chan, Y. K.
- Biotechnology Letters, Vol. 3, Issue 2
Lime Pretreatment of Sugarcane Bagasse for Bioethanol Production
journal, December 2008
- Rabelo, Sarita C.; Filho, Rubens Maciel; Costa, Aline Carvalho
- Applied Biochemistry and Biotechnology, Vol. 153, Issue 1-3
Product inhibition of cellulases studied with 14C-labeled cellulose substrates
journal, January 2013
- Teugjas, Hele; Väljamäe, Priit
- Biotechnology for Biofuels, Vol. 6, Issue 1
Directed evolution of xylose specific transporters to facilitate glucose-xylose co-utilization: Engineering Xylose Specific Transporters
journal, September 2015
- Wang, Meng; Yu, Chenzhao; Zhao, Huimin
- Biotechnology and Bioengineering, Vol. 113, Issue 3
Fermentation of undetoxified sugarcane bagasse hydrolyzates using a two stage hydrothermal and mechanical refining pretreatment
journal, August 2018
- Wang, Zhaoqin; Dien, Bruce S.; Rausch, Kent D.
- Bioresource Technology, Vol. 261
Comparative sugar recovery data from laboratory scale application of leading pretreatment technologies to corn stover
journal, December 2005
- Wyman, Charles E.; Dale, Bruce E.; Elander, Richard T.
- Bioresource Technology, Vol. 96, Issue 18, p. 2026-2032
Pretreatment: the key to unlocking low-cost cellulosic ethanol
journal, January 2008
- Yang, Bin; Wyman, Charles E.
- Biofuels, Bioproducts and Biorefining, Vol. 2, Issue 1
Strengths, challenges, and opportunities for hydrothermal pretreatment in lignocellulosic biorefineries
journal, October 2017
- Yang, Bin; Tao, Ling; Wyman, Charles E.
- Biofuels, Bioproducts and Biorefining, Vol. 12, Issue 1
Structural features affecting biomass enzymatic digestibility
journal, June 2008
- Zhu, Li; O’Dwyer, Jonathan P.; Chang, Vincent S.
- Bioresource Technology, Vol. 99, Issue 9, p. 3817-3828
Liquid hot water pretreatment of lignocellulosic biomass for bioethanol production accompanying with high valuable products
journal, January 2016
- Zhuang, Xinshu; Wang, Wen; Yu, Qiang
- Bioresource Technology, Vol. 199
Works referencing / citing this record:
Lignocellulosic biomass from agro‐industrial residues in South America: current developments and perspectives
journal, August 2019
- Magalhães, Antonio Irineudo; Carvalho, Júlio Cesar; Melo Pereira, Gilberto Vinícius
- Biofuels, Bioproducts and Biorefining, Vol. 13, Issue 6
Economic Analysis of Cellulosic Ethanol Production from Sugarcane Bagasse Using a Sequential Deacetylation, Hot Water and Disk-Refining Pretreatment
journal, September 2019
- Cheng, Ming-Hsun; Wang, Zhaoqin; Dien, Bruce S.
- Processes, Vol. 7, Issue 10, p. 642