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Title: Isolation of sugarcane bagasse hydrolyzate-tolerant yeast mutants by continuous selection

Abstract

Hemicellulose, one of the major constituents of plant cell-wall materials, comprises up to 40% of agricultural residues and hardwoods. Upon hydrolysis, hemicellulose yields a mixture of carbohydrates of which D-xylose is the major component. Hemicellulose-derived carbohydrates can easily be obtained by use of dilute acids under mild hydrolysis conditions. These sugars as well as cellulose-derived carbohydrates, are potential substrates for ethanol production. Often during acid hydrolysis many potentially toxic chemicals are formed which have been found to inhibit yeast growth and ethanol production. It is, therefore, necessary to overcome the inhibitory effect before a fermentation can be implemented. In addition to these fermentation inhibitors, salts formed as a result of neutralization of acid hydrolyzate can also affect the yeasts, thereby decreasing the fermentation rate. Previously, we have shown that ethanol can be produced from sugarcane bagasse hemicellulose hydrolyzate by a xylose-fermentating yeast, Candida species XF217, after the hydrolyzate had been treated with ion-exchange resins. This communication describes the isolation of hydrolyzate-tolerant yeast strains by a continuous adaptation and selection technique and also the growth and fermentative abilities of the strain, P11-20 in neutralized hydrolyzate.

Authors:
;
Publication Date:
Research Org.:
Purdue Univ., West Lafayette, IN
OSTI Identifier:
6407265
Report Number(s):
CONF-840509-
Journal ID: CODEN: BIBSB
Resource Type:
Conference
Journal Name:
Biotechnol. Bioeng. Symp.; (United States)
Additional Journal Information:
Journal Volume: 16:14; Conference: 6. symposium on biotechnology for fuels and chemicals, Gatlinburg, TN, USA, 15 May 1984
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; BAGASSE; ACID HYDROLYSIS; HEMICELLULOSE; YEASTS; BIOLOGICAL ADAPTATION; CELL WALL; CELLULOSE; ETHANOL; INHIBITION; PRODUCTION; SUBSTRATES; TOLERANCE; XYLOSE; AGRICULTURAL WASTES; ALCOHOLS; ALDEHYDES; CARBOHYDRATES; CELL CONSTITUENTS; CHEMICAL REACTIONS; DECOMPOSITION; FUNGI; HYDROLYSIS; HYDROXY COMPOUNDS; LYSIS; MICROORGANISMS; MONOSACCHARIDES; ORGANIC COMPOUNDS; ORGANIC WASTES; PENTOSES; PLANTS; POLYSACCHARIDES; SACCHARIDES; SOLVOLYSIS; WASTES; 090222* - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989); 140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989)

Citation Formats

Lodics, T A, and Gong, C S. Isolation of sugarcane bagasse hydrolyzate-tolerant yeast mutants by continuous selection. United States: N. p., 1984. Web.
Lodics, T A, & Gong, C S. Isolation of sugarcane bagasse hydrolyzate-tolerant yeast mutants by continuous selection. United States.
Lodics, T A, and Gong, C S. Sun . "Isolation of sugarcane bagasse hydrolyzate-tolerant yeast mutants by continuous selection". United States.
@article{osti_6407265,
title = {Isolation of sugarcane bagasse hydrolyzate-tolerant yeast mutants by continuous selection},
author = {Lodics, T A and Gong, C S},
abstractNote = {Hemicellulose, one of the major constituents of plant cell-wall materials, comprises up to 40% of agricultural residues and hardwoods. Upon hydrolysis, hemicellulose yields a mixture of carbohydrates of which D-xylose is the major component. Hemicellulose-derived carbohydrates can easily be obtained by use of dilute acids under mild hydrolysis conditions. These sugars as well as cellulose-derived carbohydrates, are potential substrates for ethanol production. Often during acid hydrolysis many potentially toxic chemicals are formed which have been found to inhibit yeast growth and ethanol production. It is, therefore, necessary to overcome the inhibitory effect before a fermentation can be implemented. In addition to these fermentation inhibitors, salts formed as a result of neutralization of acid hydrolyzate can also affect the yeasts, thereby decreasing the fermentation rate. Previously, we have shown that ethanol can be produced from sugarcane bagasse hemicellulose hydrolyzate by a xylose-fermentating yeast, Candida species XF217, after the hydrolyzate had been treated with ion-exchange resins. This communication describes the isolation of hydrolyzate-tolerant yeast strains by a continuous adaptation and selection technique and also the growth and fermentative abilities of the strain, P11-20 in neutralized hydrolyzate.},
doi = {},
journal = {Biotechnol. Bioeng. Symp.; (United States)},
number = ,
volume = 16:14,
place = {United States},
year = {1984},
month = {1}
}

Conference:
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