Correlative 3D X-ray fluorescence and ptychographic tomography of frozen-hydrated green algae

Deng, Junjing; Lo, Yuan Hung; Gallagher-Jones, Marcus; Chen, Si; Pryor, Jr., Alan; Jin, Qiaoling; Hong, Young Pyo; Nashed, Youssef S. G.; Vogt, Stefan; Miao, Jianwei; Jacobsen, Chris

doi:10.1126/sciadv.aau4548

Title: Correlative 3D X-ray fluorescence and ptychographic tomography of frozen-hydrated green algae

Journal Article · 01 November 2018 · Science Advances

DOI: https://doi.org/10.1126/sciadv.aau4548 · OSTI ID:1492393

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Argonne National Lab. (ANL), Argonne, IL (United States)
Univ. of California, Los Angeles, CA (United States)
Univ. of California, Los Angeles, CA (United States); UCLA-DOE Institute for Genomics and Proteomics, Los Angeles, CA (United States)
Northwestern Univ., Evanston, IL (United States)
Argonne National Lab. (ANL), Argonne, IL (United States); Northwestern Univ., Evanston, IL (United States)

Accurate knowledge of elemental distributions within biological organisms is critical for understanding their cellular roles. The ability to couple this knowledge with overall cellular architecture in three dimensions (3D) deepens our understanding of cellular chemistry. Using a whole, frozen-hydrated Chlamydomonas reinhardtii cell as an example, we report the development of 3D correlative microscopy through a combination of simultaneous cryogenic x-ray ptychography and x-ray fluorescence microscopy. By taking advantage of a recently developed tomographic reconstruction algorithm, termed GENeralized Fourier Iterative REconstruction (GENFIRE), we produce high-quality 3D maps of the unlabeled alga’s cellular ultrastructure and elemental distributions within the cell. We demonstrate GENFIRE’s ability to outperform conventional tomography algorithms and to further improve the reconstruction quality by refining the experimentally intended tomographic angles. As this method continues to advance with brighter coherent light sources and more efficient data handling, we expect correlative 3D x-ray fluorescence and ptychographic tomography to be a powerful tool for probing a wide range of frozen-hydrated biological specimens, ranging from small prokaryotes such as bacteria, algae, and parasites to large eukaryotes such as mammalian cells, with applications that include understanding cellular responses to environmental stimuli and cell-to-cell interactions.

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Research Organization:: Argonne National Lab. (ANL), Argonne, IL (United States)

Sponsoring Organization:: National Institutes of Health (NIH), National Institute of General Medical Sciences; National Science Foundation (NSF); USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1492393

Journal Information:: Science Advances, Journal Name: Science Advances Journal Issue: 11 Vol. 4; ISSN 2375-2548

Publisher:: AAASCopyright Statement

Country of Publication:: United States

Language:: English

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Spiral scanning X-ray fluorescence computed tomography de Jonge, Martin D.; Kingston, Andrew M.; Afshar, Nader Optics Express, Vol. 25, Issue 19 https://doi.org/10.1364/OE.25.023424	journal	January 2017
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Influence of Electron Noise on Three-dimensional Image Reconstruction Hegerl, R.; Hoppe, W. Zeitschrift für Naturforschung A, Vol. 31, Issue 12 https://doi.org/10.1515/zna-1976-1241	journal	December 1976
Ptychographic transmission microscopy in three dimensions using a multi-slice approach Maiden, A. M.; Humphry, M. J.; Rodenburg, J. M. Figshare https://doi.org/10.6084/m9.figshare.c.3753704.v1	collection	January 2012
Multi-slice ptychography with large numerical aperture multilayer Laue lenses Ozturk, Hande; Yan, Hanfei; He, Yan Figshare, vp https://doi.org/10.6084/m9.figshare.c.4042277	dataset	May 2018
Native architecture of the Chlamydomonas chloroplast revealed by in situ cryo-electron tomography Engel, Benjamin D.; Schaffer, Miroslava; Kuhn Cuellar, Luis eLife, Vol. 4 https://doi.org/10.7554/eLife.04889	journal	January 2015

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