Correlative 3D X-ray fluorescence and ptychographic tomography of frozen-hydrated green algae
Abstract
Accurate knowledge of elemental distributions within biological organisms is critical for understanding their cellular roles. The ability to couple this knowledge with overall cellular architecture in three dimensions (3D) deepens our understanding of cellular chemistry. Using a whole, frozen-hydrated Chlamydomonas reinhardtii cell as an example, we report the development of 3D correlative microscopy through a combination of simultaneous cryogenic x-ray ptychography and x-ray fluorescence microscopy. By taking advantage of a recently developed tomographic reconstruction algorithm, termed GENeralized Fourier Iterative REconstruction (GENFIRE), we produce high-quality 3D maps of the unlabeled alga’s cellular ultrastructure and elemental distributions within the cell. We demonstrate GENFIRE’s ability to outperform conventional tomography algorithms and to further improve the reconstruction quality by refining the experimentally intended tomographic angles. As this method continues to advance with brighter coherent light sources and more efficient data handling, we expect correlative 3D x-ray fluorescence and ptychographic tomography to be a powerful tool for probing a wide range of frozen-hydrated biological specimens, ranging from small prokaryotes such as bacteria, algae, and parasites to large eukaryotes such as mammalian cells, with applications that include understanding cellular responses to environmental stimuli and cell-to-cell interactions.
- Authors:
-
[1];
[2];
[3];
[1];
[4];
[4];
[1];
[1];
[2];
[5]
- Argonne National Lab. (ANL), Argonne, IL (United States)
- Univ. of California, Los Angeles, CA (United States)
- Univ. of California, Los Angeles, CA (United States); UCLA-DOE Institute for Genomics and Proteomics, Los Angeles, CA (United States)
- Northwestern Univ., Evanston, IL (United States)
- Argonne National Lab. (ANL), Argonne, IL (United States); Northwestern Univ., Evanston, IL (United States)
- Publication Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- National Science Foundation (NSF); National Institutes of Health (NIH), National Institute of General Medical Sciences; USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1492393
- Grant/Contract Number:
- AC02-06CH11357
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Science Advances
- Additional Journal Information:
- Journal Volume: 4; Journal Issue: 11; Journal ID: ISSN 2375-2548
- Publisher:
- AAAS
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Deng, Junjing, Lo, Yuan Hung, Gallagher-Jones, Marcus, Chen, Si, Pryor, Jr., Alan, Jin, Qiaoling, Hong, Young Pyo, Nashed, Youssef S. G., Vogt, Stefan, Miao, Jianwei, and Jacobsen, Chris. Correlative 3D X-ray fluorescence and ptychographic tomography of frozen-hydrated green algae. United States: N. p., 2018.
Web. doi:10.1126/sciadv.aau4548.
Deng, Junjing, Lo, Yuan Hung, Gallagher-Jones, Marcus, Chen, Si, Pryor, Jr., Alan, Jin, Qiaoling, Hong, Young Pyo, Nashed, Youssef S. G., Vogt, Stefan, Miao, Jianwei, & Jacobsen, Chris. Correlative 3D X-ray fluorescence and ptychographic tomography of frozen-hydrated green algae. United States. https://doi.org/10.1126/sciadv.aau4548
Deng, Junjing, Lo, Yuan Hung, Gallagher-Jones, Marcus, Chen, Si, Pryor, Jr., Alan, Jin, Qiaoling, Hong, Young Pyo, Nashed, Youssef S. G., Vogt, Stefan, Miao, Jianwei, and Jacobsen, Chris. Fri .
"Correlative 3D X-ray fluorescence and ptychographic tomography of frozen-hydrated green algae". United States. https://doi.org/10.1126/sciadv.aau4548. https://www.osti.gov/servlets/purl/1492393.
@article{osti_1492393,
title = {Correlative 3D X-ray fluorescence and ptychographic tomography of frozen-hydrated green algae},
author = {Deng, Junjing and Lo, Yuan Hung and Gallagher-Jones, Marcus and Chen, Si and Pryor, Jr., Alan and Jin, Qiaoling and Hong, Young Pyo and Nashed, Youssef S. G. and Vogt, Stefan and Miao, Jianwei and Jacobsen, Chris},
abstractNote = {Accurate knowledge of elemental distributions within biological organisms is critical for understanding their cellular roles. The ability to couple this knowledge with overall cellular architecture in three dimensions (3D) deepens our understanding of cellular chemistry. Using a whole, frozen-hydrated Chlamydomonas reinhardtii cell as an example, we report the development of 3D correlative microscopy through a combination of simultaneous cryogenic x-ray ptychography and x-ray fluorescence microscopy. By taking advantage of a recently developed tomographic reconstruction algorithm, termed GENeralized Fourier Iterative REconstruction (GENFIRE), we produce high-quality 3D maps of the unlabeled alga’s cellular ultrastructure and elemental distributions within the cell. We demonstrate GENFIRE’s ability to outperform conventional tomography algorithms and to further improve the reconstruction quality by refining the experimentally intended tomographic angles. As this method continues to advance with brighter coherent light sources and more efficient data handling, we expect correlative 3D x-ray fluorescence and ptychographic tomography to be a powerful tool for probing a wide range of frozen-hydrated biological specimens, ranging from small prokaryotes such as bacteria, algae, and parasites to large eukaryotes such as mammalian cells, with applications that include understanding cellular responses to environmental stimuli and cell-to-cell interactions.},
doi = {10.1126/sciadv.aau4548},
journal = {Science Advances},
number = 11,
volume = 4,
place = {United States},
year = {Fri Nov 02 00:00:00 EDT 2018},
month = {Fri Nov 02 00:00:00 EDT 2018}
}
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text, January 2015
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Three-dimensional mass density mapping of cellular ultrastructure by ptychographic X-ray nanotomography
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