X-ray ptychographic and fluorescence microscopy of frozen-hydrated cells using continuous scanning
Abstract
X-ray microscopy can be used to image whole, unsectioned cells in their native hydrated state. It complements the higher resolution of electron microscopy for submicrometer thick specimens, and the molecule-specific imaging capabilites of fluorescence light microscopy. We describe here the first use of fast, continuous x-ray scanning of frozen hydrated cells for simultaneous sub-20 nm resolution ptychographic transmission imaging with high contrast, and sub-100 nm resolution deconvolved x-ray fluorescence imaging of diffusible and bound ions at native concentrations, without the need to add specific labels. Here, by working with cells that have been rapidly frozen without the use of chemical fixatives, and imaging them under cryogenic conditions, we are able to obtain images with well preserved structural and chemical composition, and sufficient stability against radiation damage to allow for multiple images to be obtained with no observable change.
- Authors:
-
[5]
- Northwestern Univ., Evanston, IL (United States); Argonne National Lab. (ANL), Argonne, IL (United States)
- Argonne National Lab. (ANL), Argonne, IL (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Argonne National Lab. (ANL), Argonne, IL (United States)
- Northwestern Univ., Evanston, IL (United States)
- Argonne National Lab. (ANL), Argonne, IL (United States); Northwestern Univ., Evanston, IL (United States)
- Publication Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); National Institutes of Health (NIH)
- OSTI Identifier:
- 1363812
- Alternate Identifier(s):
- OSTI ID: 1411588
- Grant/Contract Number:
- AC02-06CH11357; AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Scientific Reports
- Additional Journal Information:
- Journal Volume: 7; Journal Issue: 1; Journal ID: ISSN 2045-2322
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 73 NUCLEAR PHYSICS AND RADIATION PHYSICS; microscopy; optical techniques
Citation Formats
Deng, Junjing, Vine, David J., Chen, Si, Jin, Qiaoling, Nashed, Youssef S. G., Peterka, Tom, Vogt, Stefan, and Jacobsen, Chris. X-ray ptychographic and fluorescence microscopy of frozen-hydrated cells using continuous scanning. United States: N. p., 2017.
Web. doi:10.1038/s41598-017-00569-y.
Deng, Junjing, Vine, David J., Chen, Si, Jin, Qiaoling, Nashed, Youssef S. G., Peterka, Tom, Vogt, Stefan, & Jacobsen, Chris. X-ray ptychographic and fluorescence microscopy of frozen-hydrated cells using continuous scanning. United States. https://doi.org/10.1038/s41598-017-00569-y
Deng, Junjing, Vine, David J., Chen, Si, Jin, Qiaoling, Nashed, Youssef S. G., Peterka, Tom, Vogt, Stefan, and Jacobsen, Chris. Mon .
"X-ray ptychographic and fluorescence microscopy of frozen-hydrated cells using continuous scanning". United States. https://doi.org/10.1038/s41598-017-00569-y. https://www.osti.gov/servlets/purl/1363812.
@article{osti_1363812,
title = {X-ray ptychographic and fluorescence microscopy of frozen-hydrated cells using continuous scanning},
author = {Deng, Junjing and Vine, David J. and Chen, Si and Jin, Qiaoling and Nashed, Youssef S. G. and Peterka, Tom and Vogt, Stefan and Jacobsen, Chris},
abstractNote = {X-ray microscopy can be used to image whole, unsectioned cells in their native hydrated state. It complements the higher resolution of electron microscopy for submicrometer thick specimens, and the molecule-specific imaging capabilites of fluorescence light microscopy. We describe here the first use of fast, continuous x-ray scanning of frozen hydrated cells for simultaneous sub-20 nm resolution ptychographic transmission imaging with high contrast, and sub-100 nm resolution deconvolved x-ray fluorescence imaging of diffusible and bound ions at native concentrations, without the need to add specific labels. Here, by working with cells that have been rapidly frozen without the use of chemical fixatives, and imaging them under cryogenic conditions, we are able to obtain images with well preserved structural and chemical composition, and sufficient stability against radiation damage to allow for multiple images to be obtained with no observable change.},
doi = {10.1038/s41598-017-00569-y},
journal = {Scientific Reports},
number = 1,
volume = 7,
place = {United States},
year = {Mon Mar 27 00:00:00 EDT 2017},
month = {Mon Mar 27 00:00:00 EDT 2017}
}
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