Resolving Mixtures in Solution by Single-Molecule Rotational Diffusivity
Abstract
Sensing the size of individual molecules in an ensemble has proven to be a powerful tool to investigate biomolecular interactions and association-dissociation processes. In biologically relevant solution environments, molecular size is often sensed by translational or rotational diffusivity. The rotational diffusivity is more sensitive to the size and conformation of the molecules as it is inversely proportional to the cube of the hydrodynamic radius, as opposed to the inverse linear dependence of the translational diffusion coefficient. Single-molecule rotational diffusivity has been measured with time-resolved fluorescence anisotropy decay, but the ability to sense different sizes has been restricted by the limited number of photons available or has required surface attachment to observe each molecule longer, and the attachment may be perturbative. To address these limitations, we show how to measure and monitor single-molecule rotational diffusivity by combining the solution-phase AntiBrownian ELectrokinetic (ABEL) trap and maximum likelihood analysis of time-resolved fluorescence anisotropy based on the information inherent in each detected photon. We demonstrate this approach by resolving a mixture of single- and doublestranded fluorescently labeled DNA molecules at equilibrium, freely rotating in a native solution environment. The rotational diffusivity, fluorescence brightness and lifetime, and initial and steady-state anisotropy are simultaneously determined formore »
- Authors:
-
- Department of Chemistry, Stanford University, Stanford, California 94305-4401, United States
- Publication Date:
- Research Org.:
- Stanford Univ., CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1465622
- Alternate Identifier(s):
- OSTI ID: 1481013
- Grant/Contract Number:
- FG02-07ER15892
- Resource Type:
- Published Article
- Journal Name:
- Nano Letters
- Additional Journal Information:
- Journal Name: Nano Letters Journal Volume: 18 Journal Issue: 8; Journal ID: ISSN 1530-6984
- Publisher:
- American Chemical Society
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Yang, Hsiang-Yu, and Moerner, W. E. Resolving Mixtures in Solution by Single-Molecule Rotational Diffusivity. United States: N. p., 2018.
Web. doi:10.1021/acs.nanolett.8b02280.
Yang, Hsiang-Yu, & Moerner, W. E. Resolving Mixtures in Solution by Single-Molecule Rotational Diffusivity. United States. https://doi.org/10.1021/acs.nanolett.8b02280
Yang, Hsiang-Yu, and Moerner, W. E. Thu .
"Resolving Mixtures in Solution by Single-Molecule Rotational Diffusivity". United States. https://doi.org/10.1021/acs.nanolett.8b02280.
@article{osti_1465622,
title = {Resolving Mixtures in Solution by Single-Molecule Rotational Diffusivity},
author = {Yang, Hsiang-Yu and Moerner, W. E.},
abstractNote = {Sensing the size of individual molecules in an ensemble has proven to be a powerful tool to investigate biomolecular interactions and association-dissociation processes. In biologically relevant solution environments, molecular size is often sensed by translational or rotational diffusivity. The rotational diffusivity is more sensitive to the size and conformation of the molecules as it is inversely proportional to the cube of the hydrodynamic radius, as opposed to the inverse linear dependence of the translational diffusion coefficient. Single-molecule rotational diffusivity has been measured with time-resolved fluorescence anisotropy decay, but the ability to sense different sizes has been restricted by the limited number of photons available or has required surface attachment to observe each molecule longer, and the attachment may be perturbative. To address these limitations, we show how to measure and monitor single-molecule rotational diffusivity by combining the solution-phase AntiBrownian ELectrokinetic (ABEL) trap and maximum likelihood analysis of time-resolved fluorescence anisotropy based on the information inherent in each detected photon. We demonstrate this approach by resolving a mixture of single- and doublestranded fluorescently labeled DNA molecules at equilibrium, freely rotating in a native solution environment. The rotational diffusivity, fluorescence brightness and lifetime, and initial and steady-state anisotropy are simultaneously determined for each trapped single DNA molecule. The time resolution and precision of this method are analyzed using statistical signal analysis and simulations. We present key parameters that define the usefulness of a particular fluorescent label for extracting molecular size information from single-molecule rotational diffusivity measurements.},
doi = {10.1021/acs.nanolett.8b02280},
journal = {Nano Letters},
number = 8,
volume = 18,
place = {United States},
year = {Thu Jul 12 00:00:00 EDT 2018},
month = {Thu Jul 12 00:00:00 EDT 2018}
}
https://doi.org/10.1021/acs.nanolett.8b02280
Web of Science
Works referencing / citing this record:
Time-resolved multirotational dynamics of single solution-phase tau proteins reveals details of conformational variation
journal, January 2019
- Foote, Alexander K.; Manger, Lydia H.; Holden, Michael R.
- Physical Chemistry Chemical Physics, Vol. 21, Issue 4
In situ detection of protein corona on single particle by rotational diffusivity
journal, January 2019
- Lin, Xijian; Pan, Qi; He, Yan
- Nanoscale, Vol. 11, Issue 39