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Title: Immediate drop on demand technology (I-DOT) coupled with mass spectrometry via an open port sampling interface

Abstract

Here, the immediate drop on demand technology to a mass spectrometer via the recently introduced open port sampling interface and ESI. A maximum sample analysis throughput of 5 s per sample was demonstrated. Signal reproducibility was 10% or better as demonstrated by the quantitative analysis of propranolol and its stable isotope-labeled internal standard propranolol-d7. The ability of the system to multiply charge and analyze macromolecules was demonstrated using the protein cytochrome c. In conclusion, this immediate drop on demand technology/open port sampling interface/ESI–MS combination allowed for the quantitative analysis of relatively small mass analytes and was used for the identification of macromolecules like proteins.

Authors:
ORCiD logo [1]; ORCiD logo [1];  [2]
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  2. Dispendix GmbH, Stuttgart (Germany)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1465070
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
Bioanalysis
Additional Journal Information:
Journal Volume: 9; Journal Issue: 21; Journal ID: ISSN 1757-6180
Publisher:
Future Science Group
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION; drop dispensing; electrospray ionization; immediate drop on demand; mass spectrometry; microtiter plates; noncontact dispensing; open port sampling interface

Citation Formats

Van Berkel, Gary J., Kertesz, Vilmos, and Boeltz, Harry. Immediate drop on demand technology (I-DOT) coupled with mass spectrometry via an open port sampling interface. United States: N. p., 2017. Web. doi:10.4155/bio-2017-0104.
Van Berkel, Gary J., Kertesz, Vilmos, & Boeltz, Harry. Immediate drop on demand technology (I-DOT) coupled with mass spectrometry via an open port sampling interface. United States. doi:10.4155/bio-2017-0104.
Van Berkel, Gary J., Kertesz, Vilmos, and Boeltz, Harry. Thu . "Immediate drop on demand technology (I-DOT) coupled with mass spectrometry via an open port sampling interface". United States. doi:10.4155/bio-2017-0104. https://www.osti.gov/servlets/purl/1465070.
@article{osti_1465070,
title = {Immediate drop on demand technology (I-DOT) coupled with mass spectrometry via an open port sampling interface},
author = {Van Berkel, Gary J. and Kertesz, Vilmos and Boeltz, Harry},
abstractNote = {Here, the immediate drop on demand technology to a mass spectrometer via the recently introduced open port sampling interface and ESI. A maximum sample analysis throughput of 5 s per sample was demonstrated. Signal reproducibility was 10% or better as demonstrated by the quantitative analysis of propranolol and its stable isotope-labeled internal standard propranolol-d7. The ability of the system to multiply charge and analyze macromolecules was demonstrated using the protein cytochrome c. In conclusion, this immediate drop on demand technology/open port sampling interface/ESI–MS combination allowed for the quantitative analysis of relatively small mass analytes and was used for the identification of macromolecules like proteins.},
doi = {10.4155/bio-2017-0104},
journal = {Bioanalysis},
number = 21,
volume = 9,
place = {United States},
year = {2017},
month = {11}
}

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