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Title: Combined Falling Drop/Open Port Sampling Interface System for Automated Flow Injection Mass Spectrometry

The aim of this work was to demonstrate and to evaluate the analytical performance of a combined falling drop/open port sampling interface (OPSI) system as a simple noncontact, no-carryover, automated system for flow injection analysis with mass spectrometry. The falling sample drops were introduced into the OPSI using a widely available autosampler platform utilizing low cost disposable pipet tips and conventional disposable microtiter well plates. The volume of the drops that fell onto the OPSI was in the 7–15 μL range with an injected sample volume of several hundred nanoliters. Sample drop height, positioning of the internal capillary on the sampling end of the probe, and carrier solvent flow rate were optimized for maximum signal. Sample throughput, signal reproducibility, matrix effects, and quantitative analysis capability of the system were established using the drug molecule propranolol and its isotope labeled internal standard in water, unprocessed river water and two commercially available buffer matrices. A sample-to-sample throughput of ~45 s with a ~4.5 s base-to-base flow injection peak profile was obtained in these experiments. In addition, quantitation with minimally processed rat plasma samples was demonstrated with three different statin drugs (atorvastatin, rosuvastatin, and fluvastatin). Direct characterization capability of unprocessed samples was demonstratedmore » by the analysis of neat vegetable oils. Employing the autosampler system for spatially resolved liquid extraction surface sampling exemplified by the analysis of propranolol and its hydroxypropranolol glucuronide phase II metabolites from a rat thin tissue section was also illustrated.« less
Authors:
ORCiD logo [1] ; ORCiD logo [1] ;  [2] ;  [3] ;  [3] ;  [3]
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  2. Resolution Labs, New Haven, IN (United States)
  3. Novartis Inst. for Biomedical Sciences Drug Metabolism & Pharmacokinetics, East Hanover, NJ (United States)
Publication Date:
Grant/Contract Number:
AC05-00OR22725
Type:
Accepted Manuscript
Journal Name:
Analytical Chemistry
Additional Journal Information:
Journal Volume: 89; Journal Issue: 22; Journal ID: ISSN 0003-2700
Publisher:
American Chemical Society (ACS)
Research Org:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION
OSTI Identifier:
1435291

Van Berkel, Gary J., Kertesz, Vilmos, Orcutt, Matt, Bentley, Adam, Glick, Jim, and Flarakos, Jimmy. Combined Falling Drop/Open Port Sampling Interface System for Automated Flow Injection Mass Spectrometry. United States: N. p., Web. doi:10.1021/acs.analchem.7b03899.
Van Berkel, Gary J., Kertesz, Vilmos, Orcutt, Matt, Bentley, Adam, Glick, Jim, & Flarakos, Jimmy. Combined Falling Drop/Open Port Sampling Interface System for Automated Flow Injection Mass Spectrometry. United States. doi:10.1021/acs.analchem.7b03899.
Van Berkel, Gary J., Kertesz, Vilmos, Orcutt, Matt, Bentley, Adam, Glick, Jim, and Flarakos, Jimmy. 2017. "Combined Falling Drop/Open Port Sampling Interface System for Automated Flow Injection Mass Spectrometry". United States. doi:10.1021/acs.analchem.7b03899. https://www.osti.gov/servlets/purl/1435291.
@article{osti_1435291,
title = {Combined Falling Drop/Open Port Sampling Interface System for Automated Flow Injection Mass Spectrometry},
author = {Van Berkel, Gary J. and Kertesz, Vilmos and Orcutt, Matt and Bentley, Adam and Glick, Jim and Flarakos, Jimmy},
abstractNote = {The aim of this work was to demonstrate and to evaluate the analytical performance of a combined falling drop/open port sampling interface (OPSI) system as a simple noncontact, no-carryover, automated system for flow injection analysis with mass spectrometry. The falling sample drops were introduced into the OPSI using a widely available autosampler platform utilizing low cost disposable pipet tips and conventional disposable microtiter well plates. The volume of the drops that fell onto the OPSI was in the 7–15 μL range with an injected sample volume of several hundred nanoliters. Sample drop height, positioning of the internal capillary on the sampling end of the probe, and carrier solvent flow rate were optimized for maximum signal. Sample throughput, signal reproducibility, matrix effects, and quantitative analysis capability of the system were established using the drug molecule propranolol and its isotope labeled internal standard in water, unprocessed river water and two commercially available buffer matrices. A sample-to-sample throughput of ~45 s with a ~4.5 s base-to-base flow injection peak profile was obtained in these experiments. In addition, quantitation with minimally processed rat plasma samples was demonstrated with three different statin drugs (atorvastatin, rosuvastatin, and fluvastatin). Direct characterization capability of unprocessed samples was demonstrated by the analysis of neat vegetable oils. Employing the autosampler system for spatially resolved liquid extraction surface sampling exemplified by the analysis of propranolol and its hydroxypropranolol glucuronide phase II metabolites from a rat thin tissue section was also illustrated.},
doi = {10.1021/acs.analchem.7b03899},
journal = {Analytical Chemistry},
number = 22,
volume = 89,
place = {United States},
year = {2017},
month = {11}
}