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Title: Observation of yttrium oxide nanoparticles in cabbage (Brassica oleracea) through dual energy K-edge subtraction imaging

Abstract

Background: The potential transfer of engineered nanoparticles (ENPs) from plants into the food chain has raised widespread concerns. In order to investigate the effects of ENPs on plants, young cabbage plants (Brassica oleracea) were exposed to a hydroponic system containing yttrium oxide (yttria) ENPs. The objective of this study was to reveal the impacts of NPs on plants by using K-edge subtraction imaging technique. Results: Using synchrotron dual-e nergy X-ray micro-tomography with K-edge subtraction technique, we studied the uptake, accumulation, distribution and concentration mapping of yttria ENPs in cabbage plants. It was found that yttria ENPs were uptaken by the cabbage roots but did not effectively transferred and mobilized through the cabbage stem and leaves. This could be due to the accumulation of yttria ENPs blocked at primary-lateral-root junction. Instead, non-yttria minerals were found in the xylem vessels of roots and stem. Conclusions: Synchrotron dual-energy X-ray micro-tomography is an effective method to observe yttria NPs inside the cabbage plants in both whole body and microscale level. Furthermore, the blockage of a plant's roots by nanoparticles is likely the first and potentially fatal environmental effect of such type of nanoparticles.

Authors:
 [1];  [2];  [1];  [3];  [3];  [4];  [5]
  1. Texas A & M Univ., College Station, TX (United States). Materials Science and Engineering
  2. Texas A & M Univ., College Station, TX (United States). Mechanical Engineering
  3. California State Univ., Fresno, CA (United States). Dept. of Plant Science
  4. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Advanced Light Source
  5. Texas A & M Univ., College Station, TX (United States). Materials Science and Engineering, Mechanical Engineering
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1379247
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
Journal of Nanobiotechnology
Additional Journal Information:
Journal Volume: 14; Journal Issue: 1; Journal ID: ISSN 1477-3155
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; synchrotron x-ray micro-tomography; k-edge subtraction imaging; ytrria nanoparticles; cabbage; acumulation

Citation Formats

Chen, Yunyun, Sanchez, Carlos, Yue, Yuan, de Almeida, Mauricio, González, Jorge M., Parkinson, Dilworth Y., and Liang, Hong. Observation of yttrium oxide nanoparticles in cabbage (Brassica oleracea) through dual energy K-edge subtraction imaging. United States: N. p., 2016. Web. doi:10.1186/s12951-016-0175-z.
Chen, Yunyun, Sanchez, Carlos, Yue, Yuan, de Almeida, Mauricio, González, Jorge M., Parkinson, Dilworth Y., & Liang, Hong. Observation of yttrium oxide nanoparticles in cabbage (Brassica oleracea) through dual energy K-edge subtraction imaging. United States. doi:10.1186/s12951-016-0175-z.
Chen, Yunyun, Sanchez, Carlos, Yue, Yuan, de Almeida, Mauricio, González, Jorge M., Parkinson, Dilworth Y., and Liang, Hong. Fri . "Observation of yttrium oxide nanoparticles in cabbage (Brassica oleracea) through dual energy K-edge subtraction imaging". United States. doi:10.1186/s12951-016-0175-z. https://www.osti.gov/servlets/purl/1379247.
@article{osti_1379247,
title = {Observation of yttrium oxide nanoparticles in cabbage (Brassica oleracea) through dual energy K-edge subtraction imaging},
author = {Chen, Yunyun and Sanchez, Carlos and Yue, Yuan and de Almeida, Mauricio and González, Jorge M. and Parkinson, Dilworth Y. and Liang, Hong},
abstractNote = {Background: The potential transfer of engineered nanoparticles (ENPs) from plants into the food chain has raised widespread concerns. In order to investigate the effects of ENPs on plants, young cabbage plants (Brassica oleracea) were exposed to a hydroponic system containing yttrium oxide (yttria) ENPs. The objective of this study was to reveal the impacts of NPs on plants by using K-edge subtraction imaging technique. Results: Using synchrotron dual-e nergy X-ray micro-tomography with K-edge subtraction technique, we studied the uptake, accumulation, distribution and concentration mapping of yttria ENPs in cabbage plants. It was found that yttria ENPs were uptaken by the cabbage roots but did not effectively transferred and mobilized through the cabbage stem and leaves. This could be due to the accumulation of yttria ENPs blocked at primary-lateral-root junction. Instead, non-yttria minerals were found in the xylem vessels of roots and stem. Conclusions: Synchrotron dual-energy X-ray micro-tomography is an effective method to observe yttria NPs inside the cabbage plants in both whole body and microscale level. Furthermore, the blockage of a plant's roots by nanoparticles is likely the first and potentially fatal environmental effect of such type of nanoparticles.},
doi = {10.1186/s12951-016-0175-z},
journal = {Journal of Nanobiotechnology},
number = 1,
volume = 14,
place = {United States},
year = {2016},
month = {3}
}

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    Works referencing / citing this record:

    High efficiency upconversion nanophosphors for high-contrast bioimaging
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