Efficacy of atmospheric pressure dielectric barrier discharge for inactivating airborne pathogens

Romero-Mangado, Jaione; Dey, Avishek; Diaz-Cartagena, Diana C.; Solis-Marcano, Nadja E.; López-Nieves, Marjorie; Santiago-García, Vilynette; Nordlund, Dennis; Krishnamurthy, Satheesh; Meyyappan, M.; Koehne, Jessica E.; Gandhiraman, Ram P.

doi:10.1116/1.4990654

Title: Efficacy of atmospheric pressure dielectric barrier discharge for inactivating airborne pathogens

Abstract

Atmospheric pressure plasmas have gained attention in recent years for several environmental applications. This technology could potentially be used to deactivate airborne microorganisms, surface-bound microorganisms, and biofilms. Here, the authors explore the efficacy of the atmospheric pressure dielectric barrier discharge (DBD) to inactivate airborne Staphylococcus epidermidis and Aspergillus niger that are opportunistic pathogens associated with nosocomial infections. This technology uses air as the source of gas and does not require any process gas such as helium, argon, nitrogen, or hydrogen. Moreover, the effect of DBD was studied on aerosolized S. epidermidis and aerosolized A. niger spores via scanning electron microscopy (SEM). The morphology observed on the SEM micrographs showed deformations in the cellular structure of both microorganisms. Cell structure damage upon interaction with the DBD suggests leakage of vital cellular materials, which is a key mechanism for microbial inactivation. The chemical structure of the cell surface of S. epidermidis was also analyzed by near edge x-ray absorption fine structure spectroscopy before and after DBD exposure. Our results from surface analysis revealed that reactive oxygen species from the DBD discharge contributed to alterations on the chemistry of the cell membrane/cell wall of S. epidermidis.

Authors:

Romero-Mangado, Jaione ^[1]; Dey, Avishek ^[2]; Diaz-Cartagena, Diana C. ^[3]; Solis-Marcano, Nadja E. ^[3]; López-Nieves, Marjorie ^[3]; Santiago-García, Vilynette ^[3]; Nordlund, Dennis ^[4]; Krishnamurthy, Satheesh ^[5]; Meyyappan, M. ^[6]; Koehne, Jessica E. ^[6]; Gandhiraman, Ram P. ^[7]

Science and Technology Corporation, Moffett Field, CA (United States)
Open Univ., Milton Keynes (United Kingdom). Universities Space Research Association, Materials Engineering
Univ. of Puerto Rico, San Juan, PR (United States). Dept. of Chemistry
SLAC National Accelerator Lab., Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource
Open Univ. Milton Keynes (United Kingdom). Materials Engineering
NASA Ames Research Center (ARC), Moffett Field, Mountain View, CA (United States)
Universities Space Research Association, Mountain View, CA (United States)

Publication Date:: Wed Jul 05 00:00:00 EDT 2017

Research Org.:: SLAC National Accelerator Lab., Menlo Park, CA (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

OSTI Identifier:: 1374386

Grant/Contract Number:: AC02-76SF00515

Resource Type:: Accepted Manuscript

Journal Name:: Journal of Vacuum Science and Technology A

Additional Journal Information:: Journal Volume: 35; Journal Issue: 4; Journal ID: ISSN 0734-2101

Publisher:: American Vacuum Society

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Citation Formats


                    Romero-Mangado, Jaione, Dey, Avishek, Diaz-Cartagena, Diana C., Solis-Marcano, Nadja E., López-Nieves, Marjorie, Santiago-García, Vilynette, Nordlund, Dennis, Krishnamurthy, Satheesh, Meyyappan, M., Koehne, Jessica E., and Gandhiraman, Ram P. Efficacy of atmospheric pressure dielectric barrier discharge for inactivating airborne pathogens.  United States: N. p., 2017. 
Web.  doi:10.1116/1.4990654.

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                    Romero-Mangado, Jaione, Dey, Avishek, Diaz-Cartagena, Diana C., Solis-Marcano, Nadja E., López-Nieves, Marjorie, Santiago-García, Vilynette, Nordlund, Dennis, Krishnamurthy, Satheesh, Meyyappan, M., Koehne, Jessica E., & Gandhiraman, Ram P. Efficacy of atmospheric pressure dielectric barrier discharge for inactivating airborne pathogens.  United States.  https://doi.org/10.1116/1.4990654

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                    Romero-Mangado, Jaione, Dey, Avishek, Diaz-Cartagena, Diana C., Solis-Marcano, Nadja E., López-Nieves, Marjorie, Santiago-García, Vilynette, Nordlund, Dennis, Krishnamurthy, Satheesh, Meyyappan, M., Koehne, Jessica E., and Gandhiraman, Ram P. Wed .  
"Efficacy of atmospheric pressure dielectric barrier discharge for inactivating airborne pathogens".  United States.  https://doi.org/10.1116/1.4990654.  https://www.osti.gov/servlets/purl/1374386.

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@article{osti_1374386,

  title        = {Efficacy of atmospheric pressure dielectric barrier discharge for inactivating airborne pathogens},

  author       = {Romero-Mangado, Jaione and Dey, Avishek and Diaz-Cartagena, Diana C. and Solis-Marcano, Nadja E. and López-Nieves, Marjorie and Santiago-García, Vilynette and Nordlund, Dennis and Krishnamurthy, Satheesh and Meyyappan, M. and Koehne, Jessica E. and Gandhiraman, Ram P.},

  abstractNote = {Atmospheric pressure plasmas have gained attention in recent years for several environmental applications. This technology could potentially be used to deactivate airborne microorganisms, surface-bound microorganisms, and biofilms. Here, the authors explore the efficacy of the atmospheric pressure dielectric barrier discharge (DBD) to inactivate airborne Staphylococcus epidermidis and Aspergillus niger that are opportunistic pathogens associated with nosocomial infections. This technology uses air as the source of gas and does not require any process gas such as helium, argon, nitrogen, or hydrogen. Moreover, the effect of DBD was studied on aerosolized S. epidermidis and aerosolized A. niger spores via scanning electron microscopy (SEM). The morphology observed on the SEM micrographs showed deformations in the cellular structure of both microorganisms. Cell structure damage upon interaction with the DBD suggests leakage of vital cellular materials, which is a key mechanism for microbial inactivation. The chemical structure of the cell surface of S. epidermidis was also analyzed by near edge x-ray absorption fine structure spectroscopy before and after DBD exposure. Our results from surface analysis revealed that reactive oxygen species from the DBD discharge contributed to alterations on the chemistry of the cell membrane/cell wall of S. epidermidis.},

  doi          = {10.1116/1.4990654},

  journal      = {Journal of Vacuum Science and Technology A},

  number       = 4,

  volume       = 35,

  place        = {United States},

  year         = {Wed Jul 05 00:00:00 EDT 2017},

  month        = {Wed Jul 05 00:00:00 EDT 2017}

}

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Title: Efficacy of atmospheric pressure dielectric barrier discharge for inactivating airborne pathogens

Abstract

Citation Formats

Microwave micro torch generated in argon based mixtures for biomedical applications journal, August 2018

Microwave micro torch generated in argon based mixtures for biomedical applications
journal, August 2018