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Title: Morphological and chemical changes of aerosolized E. coli treated with a dielectric barrier discharge

This paper presents the morphological and chemical modification of the cell structure of aerosolized Escherichia coli treated with a dielectric barrier discharge (DBD). Exposure to DBD results in severe oxidation of the bacteria, leading to the formation of hydroxyl groups and carbonyl groups and a significant reduction in amine functionalities and phosphate groups. Near edge x-ray absorption fine structure(NEXAFS) measurements confirm the presence of additional oxide bonds upon DBD treatment, suggesting oxidation of the outer layer of the cell wall. Electron microscopy images show that the bacteria undergo physical distortion to varying degrees, resulting in deformation of the bacterial structure. The electromagnetic field around the DBD coil causes severe damage to the cell structure, possibly resulting in leakage of vital cellular materials. The oxidation and chemical modification of the bacterial components are evident from the Fourier transform infrared spectroscopy and NEXAFS results. The bacterial reculture experiments confirm inactivation of airborne E. coli upon treating with DBD.
 [1] ;  [2] ;  [3] ;  [3] ;  [3] ;  [4] ;  [5] ;  [5] ;  [1] ;  [1] ;  [1] ;  [1] ;  [6]
  1. NASA Ames Research Center, Moffett Field, CA (United States)
  2. SLAC National Accelerator Lab., Menlo Park, CA (United States)
  3. Novaerus, Inc., Chicago, IL (United States)
  4. Aalto Univ., Espoo (Finland)
  5. Dublin City Univ., Dublin (Ireland)
  6. NASA Ames Research Center, Moffett Field, CA (United States); Univ. Space Research Association, Mountain View, CA (United States)
Publication Date:
OSTI Identifier:
Grant/Contract Number:
Accepted Manuscript
Journal Name:
Additional Journal Information:
Journal Volume: 11; Journal Issue: 1; Journal ID: ISSN 1934-8630
Research Org:
SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
59 BASIC BIOLOGICAL SCIENCES bacteria; X-ray absorption spectroscopy; X-ray absorption near edge structure; carbon; Fourier transform infrared spectroscopy