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Title: Conversion of corn stover alkaline pre-treatment waste streams into biodiesel via Rhodococci

We present the bioconversion of second-generation cellulosic ethanol waste streams into biodiesel via oleaginous bacteria is a novel optimization strategy for biorefineries with substantial potential for rapid development. In this study, one- and two-stage alkali/alkali-peroxide pretreatment waste streams of corn stover were separately implemented as feedstocks in 96 h batch reactor fermentations with wild-type Rhodococcus opacus PD 630, R. opacus DSM 1069, and R. jostii DSM 44719 T . Here we show using 31P-NMR, HPAECPAD, and SEC analyses, that the more rigorous and chemically-efficient two-stage chemical pretreatment effluent provided higher concentrations of solubilized glucose and lower molecular weight (70 300 g mol1 ) lignin degradation products thereby enabling improved cellular density, viability, and oleaginicity in each respective strain. The most significant yields were by R. opacus PD 630, which converted 6.2% of organic content with a maximal total lipid production of 1.3 g L1 and accumulated 42.1% in oils based on cell dry weight after 48 h.
Authors:
 [1] ;  [1] ;  [1] ;  [1] ;  [2] ;  [3] ;  [4] ;  [5] ;  [6]
  1. Univ. of Tennessee, Knoxville, TN (United States). Department of Chemical & Biomolecular Engineering; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Bioscience Division
  2. Michigan State Univ., East Lansing, MI (United States). Department of Chemical Engineering & Materials Science and Great Lakes Bioenergy Research Center
  3. Michigan State Univ., East Lansing, MI (United States). Great Lakes Bioenergy Research Center and Department of Biochemistry
  4. Michigan State Univ., East Lansing, MI (United States). Department of Chemical Engineering & Materials Science, Great Lakes Bioenergy Research Center and Department of Biosystems & Agricultural Engineering
  5. Texas A & M Univ., College Station, TX (United States). Synthetic and Systems Biology Innovation Hub, Department of Plant Pathology and Microbiology
  6. Univ. of Tennessee, Knoxville, TN (United States). Department of Chemical & Biomolecular Engineering and Department of Forestry, Wildlife, and Fisheries, Center for Renewable Carbon; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Bioscience Division
Publication Date:
Grant/Contract Number:
AC05-00OR22725; EE0006112
Type:
Accepted Manuscript
Journal Name:
RSC Advances
Additional Journal Information:
Journal Volume: 7; Journal Issue: 7; Journal ID: ISSN 2046-2069
Publisher:
Royal Society of Chemistry
Research Org:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Joint Institute for Biological Sciences (JIBS)
Sponsoring Org:
USDOE Office of Science (SC)
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
OSTI Identifier:
1356963