Conversion of corn stover alkaline pre-treatment waste streams into biodiesel via Rhodococci

Le, Rosemary K.; Wells Jr., Tyrone; Das, Parthapratim; Meng, Xianzhi; Stoklosa, Ryan J.; Bhalla, Aditya; Hodge, David B.; Yuan, Joshua S.; Ragauskas, Arthur J.

doi:10.1039/c6ra28033a

Title: Conversion of corn stover alkaline pre-treatment waste streams into biodiesel via Rhodococci

Journal Article · 12 January 2017 · RSC Advances

DOI: https://doi.org/10.1039/c6ra28033a · OSTI ID:1356963

Le, Rosemary K. ^[1]; Wells Jr., Tyrone ^[1]; Das, Parthapratim ^[1]; Meng, Xianzhi ^[1]; Stoklosa, Ryan J. ^[2]; Bhalla, Aditya ^[3]; Hodge, David B. ^[4]; Yuan, Joshua S. ^[5]; Ragauskas, Arthur J. ^[6]

Univ. of Tennessee, Knoxville, TN (United States). Department of Chemical & Biomolecular Engineering; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Bioscience Division
Michigan State Univ., East Lansing, MI (United States). Department of Chemical Engineering & Materials Science and Great Lakes Bioenergy Research Center
Michigan State Univ., East Lansing, MI (United States). Great Lakes Bioenergy Research Center and Department of Biochemistry
Michigan State Univ., East Lansing, MI (United States). Department of Chemical Engineering & Materials Science, Great Lakes Bioenergy Research Center and Department of Biosystems & Agricultural Engineering
Texas A & M Univ., College Station, TX (United States). Synthetic and Systems Biology Innovation Hub, Department of Plant Pathology and Microbiology
Univ. of Tennessee, Knoxville, TN (United States). Department of Chemical & Biomolecular Engineering and Department of Forestry, Wildlife, and Fisheries, Center for Renewable Carbon; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Bioscience Division

We present the bioconversion of second-generation cellulosic ethanol waste streams into biodiesel via oleaginous bacteria is a novel optimization strategy for biorefineries with substantial potential for rapid development. In this study, one- and two-stage alkali/alkali-peroxide pretreatment waste streams of corn stover were separately implemented as feedstocks in 96 h batch reactor fermentations with wild-type Rhodococcus opacus PD 630, R. opacus DSM 1069, and R. jostii DSM 44719^T . Here we show using ³¹P-NMR, HPAECPAD, and SEC analyses, that the more rigorous and chemically-efficient two-stage chemical pretreatment effluent provided higher concentrations of solubilized glucose and lower molecular weight (70 300 g mol1 ) lignin degradation products thereby enabling improved cellular density, viability, and oleaginicity in each respective strain. The most significant yields were by R. opacus PD 630, which converted 6.2% of organic content with a maximal total lipid production of 1.3 g L1 and accumulated 42.1% in oils based on cell dry weight after 48 h.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Joint Institute for Biological Sciences (JIBS); Texas A&M AgriLife Research, College Station, TX (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Office of Sustainable Transportation. Bioenergy Technologies Office (BETO); USDOE Office of Science (SC)

Grant/Contract Number:: AC05-00OR22725; EE0006112; EE0007104

OSTI ID:: 1356963

Journal Information:: RSC Advances, Journal Name: RSC Advances Journal Issue: 7 Vol. 7; ISSN 2046-2069; ISSN RSCACL

Publisher:: Royal Society of ChemistryCopyright Statement

Country of Publication:: United States

Language:: English

References (50)

Production of Biofuels from Cellulose and Corn Stover Using Alkylphenol Solvents Alonso, David Martin; Wettstein, Stephanie G.; Bond, Jesse Q. ChemSusChem, Vol. 4, Issue 8 https://doi.org/10.1002/cssc.201100256	journal	June 2011
Triacylglycerols in prokaryotic microorganisms H., Alvarez; A., Steinbüchel Applied Microbiology and Biotechnology, Vol. 60, Issue 4 https://doi.org/10.1007/s00253-002-1135-0	journal	December 2002
Bioconversion of lignin model compounds with oleaginous Rhodococci Kosa, Matyas; Ragauskas, Arthur J. Applied Microbiology and Biotechnology, Vol. 93, Issue 2 https://doi.org/10.1007/s00253-011-3743-z	journal	December 2011
Accumulation and mobilization of storage lipids by Rhodococcus opacus PD630 and Rhodococcus ruber NCIMB 40126 Alvarez, H. M.; Kalscheuer, R.; Steinbüchel, A. Applied Microbiology and Biotechnology, Vol. 54, Issue 2 https://doi.org/10.1007/s002530000395	journal	August 2000
Policies for the Sustainable Development of Biofuels in the Pan American Region: A Review and Synthesis of Five Countries Solomon, Barry D.; Banerjee, Aparajita; Acevedo, Alberto Environmental Management, Vol. 56, Issue 6 https://doi.org/10.1007/s00267-014-0424-6	journal	December 2014
Biofuels and Climate Change Mitigation: A CGE Analysis Incorporating Land-Use Change Timilsina, Govinda R.; Mevel, Simon Environmental and Resource Economics, Vol. 55, Issue 1 https://doi.org/10.1007/s10640-012-9609-8	journal	October 2012
Ethanol Production Using Corn, Switchgrass, and Wood; Biodiesel Production Using Soybean and Sunflower Pimentel, David; Patzek, Tad W. Natural Resources Research, Vol. 14, Issue 1 https://doi.org/10.1007/s11053-005-4679-8	journal	March 2005
Pyrolysis Oil-Based Lipid Production as Biodiesel Feedstock by Rhodococcus opacus Wei, Zhen; Zeng, Guangming; Kosa, Matyas Applied Biochemistry and Biotechnology, Vol. 175, Issue 2 https://doi.org/10.1007/s12010-014-1305-4	journal	November 2014
Availability of corn stover as a sustainable feedstock for bioethanol production Kadam, Kiran L.; McMillan, James D. Bioresource Technology, Vol. 88, Issue 1 https://doi.org/10.1016/S0960-8524(02)00269-9	journal	May 2003
Potential of using organic fertilizer to cultivate Chlorella vulgaris for biodiesel production Lam, Man Kee; Lee, Keat Teong Applied Energy, Vol. 94 https://doi.org/10.1016/j.apenergy.2012.01.075	journal	June 2012
Bioconversion of lignocellulosic pretreatment effluent via oleaginous Rhodococcus opacus DSM 1069 Wells, Tyrone; Wei, Zhen; Ragauskas, Arthur Biomass and Bioenergy, Vol. 72 https://doi.org/10.1016/j.biombioe.2014.11.004	journal	January 2015
Biodiesel production from heterotrophic microalgal oil Miao, Xiaoling; Wu, Qingyu Bioresource Technology, Vol. 97, Issue 6 https://doi.org/10.1016/j.biortech.2005.04.008	journal	April 2006
An overview of second generation biofuel technologies Sims, Ralph E. H.; Mabee, Warren; Saddler, Jack N. Bioresource Technology, Vol. 101, Issue 6, p. 1570-1580 https://doi.org/10.1016/j.biortech.2009.11.046	journal	March 2010
Cultivation of lipid-producing bacteria with lignocellulosic biomass: Effects of inhibitory compounds of lignocellulosic hydrolysates Wang, Baixin; Rezenom, Yohannes H.; Cho, Kun-Ching Bioresource Technology, Vol. 161 https://doi.org/10.1016/j.biortech.2014.02.133	journal	June 2014
A review on alkaline pretreatment technology for bioconversion of lignocellulosic biomass Kim, Jun Seok; Lee, Y. Y.; Kim, Tae Hyun Bioresource Technology, Vol. 199 https://doi.org/10.1016/j.biortech.2015.08.085	journal	January 2016
Converting paper mill sludge into neutral lipids by oleaginous yeast Cryptococcus vishniaccii for biodiesel production Deeba, Farha; Pruthi, Vikas; Negi, Yuvraj S. Bioresource Technology, Vol. 213 https://doi.org/10.1016/j.biortech.2016.02.105	journal	August 2016
Biodiesel from microalgae Chisti, Yusuf Biotechnology Advances, Vol. 25, Issue 3, p. 294-306 https://doi.org/10.1016/j.biotechadv.2007.02.001	journal	May 2007
Alkaline peroxide pretreatment for efficient enzymatic saccharification of bamboo Yamashita, Yuya; Shono, Megumi; Sasaki, Chizuru Carbohydrate Polymers, Vol. 79, Issue 4 https://doi.org/10.1016/j.carbpol.2009.10.017	journal	March 2010
Opportunities and challenges in biological lignin valorization Beckham, Gregg T.; Johnson, Christopher W.; Karp, Eric M. Current Opinion in Biotechnology, Vol. 42 https://doi.org/10.1016/j.copbio.2016.02.030	journal	December 2016
High-cell-density batch fermentation of Rhodococcus opacus PD630 using a high glucose concentration for triacylglycerol production Kurosawa, Kazuhiko; Boccazzi, Paolo; de Almeida, Naomi M. Journal of Biotechnology, Vol. 147, Issue 3-4 https://doi.org/10.1016/j.jbiotec.2010.04.003	journal	June 2010
Biodiesel production from oleaginous microorganisms Meng, Xin; Yang, Jianming; Xu, Xin Renewable Energy, Vol. 34, Issue 1 https://doi.org/10.1016/j.renene.2008.04.014	journal	January 2009
A regulatory policy to promote renewable energy consumption in China: Review and future evolutionary path He, Yongxiu; Xu, Yang; Pang, Yuexia Renewable Energy, Vol. 89 https://doi.org/10.1016/j.renene.2015.12.047	journal	April 2016
A comprehensive review on biodiesel as an alternative energy resource and its characteristics Atabani, A. E.; Silitonga, A. S.; Badruddin, Irfan Anjum Renewable and Sustainable Energy Reviews, Vol. 16, Issue 4 https://doi.org/10.1016/j.rser.2012.01.003	journal	May 2012
Sustainable bio-ethanol production from agro-residues: A review Gupta, Anubhuti; Verma, Jay Prakash Renewable and Sustainable Energy Reviews, Vol. 41 https://doi.org/10.1016/j.rser.2014.08.032	journal	January 2015
A review of the combustion and emissions properties of advanced transportation biofuels and their impact on existing and future engines Bergthorson, Jeffrey M.; Thomson, Murray J. Renewable and Sustainable Energy Reviews, Vol. 42 https://doi.org/10.1016/j.rser.2014.10.034	journal	February 2015
Environmental sustainability of bioethanol production from rice straw in India: A review Singh, Renu; Srivastava, Monika; Shukla, Ashish Renewable and Sustainable Energy Reviews, Vol. 54 https://doi.org/10.1016/j.rser.2015.10.005	journal	February 2016
A comparative review of China, India and Pakistan renewable energy sectors and sharing opportunities Ahmed, Saeed; Mahmood, Anzar; Hasan, Ahmad Renewable and Sustainable Energy Reviews, Vol. 57 https://doi.org/10.1016/j.rser.2015.12.191	journal	May 2016
Biotechnological opportunities with the β-ketoadipate pathway Wells, Tyrone; Ragauskas, Arthur J. Trends in Biotechnology, Vol. 30, Issue 12 https://doi.org/10.1016/j.tibtech.2012.09.008	journal	December 2012
Polymerization of Kraft lignin via ultrasonication for high-molecular-weight applications Wells, Tyrone; Kosa, Matyas; Ragauskas, Arthur J. Ultrasonics Sonochemistry, Vol. 20, Issue 6 https://doi.org/10.1016/j.ultsonch.2013.05.001	journal	November 2013
Alkaline Pretreatment of Switchgrass Karp, Eric M.; Resch, Michael G.; Donohoe, Bryon S. ACS Sustainable Chemistry & Engineering, Vol. 3, Issue 7 https://doi.org/10.1021/acssuschemeng.5b00201	journal	May 2015
Base-Catalyzed Depolymerization of Biorefinery Lignins Katahira, Rui; Mittal, Ashutosh; McKinney, Kellene ACS Sustainable Chemistry & Engineering, Vol. 4, Issue 3 https://doi.org/10.1021/acssuschemeng.5b01451	journal	December 2015
Ethanol Production from Alkaline Peroxide Pretreated Enzymatically Saccharified Wheat Straw Saha, B. C.; Cotta, M. A. Biotechnology Progress, Vol. 22, Issue 2 https://doi.org/10.1021/bp050310r	journal	April 2006
Chemical Structures of Corn Stover and Its Residue after Dilute Acid Prehydrolysis and Enzymatic Hydrolysis: Insight into Factors Limiting Enzymatic Hydrolysis Mao, J. -D.; Holtman, K. M.; Franqui-Villanueva, D. Journal of Agricultural and Food Chemistry, Vol. 58, Issue 22 https://doi.org/10.1021/jf102514r	journal	November 2010
Sustainable bioenergy production from marginal lands in the US Midwest Gelfand, Ilya; Sahajpal, Ritvik; Zhang, Xuesong Nature, Vol. 493, Issue 7433 https://doi.org/10.1038/nature11811	journal	January 2013
Fuelling the future: microbial engineering for the production of sustainable biofuels Liao, James C.; Mi, Luo; Pontrelli, Sammy Nature Reviews Microbiology, Vol. 14, Issue 5 https://doi.org/10.1038/nrmicro.2016.32	journal	March 2016
Production of FAME biodiesel in E. coli by direct methylation with an insect enzyme Sherkhanov, Saken; Korman, Tyler P.; Clarke, Steven G. Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep24239	journal	April 2016
Characterization of key triacylglycerol biosynthesis processes in rhodococci Amara, Sawsan; Seghezzi, Nicolas; Otani, Hiroshi Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep24985	journal	April 2016
From sugars to biodiesel using microalgae and yeast Gomez, Jose A.; Höffner, Kai; Barton, Paul I. Green Chemistry, Vol. 18, Issue 2 https://doi.org/10.1039/C5GC01843A	journal	January 2016
Application of quantitative 31P NMR in biomass lignin and biofuel precursors characterization Pu, Yunqiao; Cao, Shilin; Ragauskas, Arthur J. Energy & Environmental Science, Vol. 4, Issue 9 https://doi.org/10.1039/c1ee01201k	journal	January 2011
The role of biofuels in the future energy supply Caspeta, Luis; Buijs, Nicolaas A. A.; Nielsen, Jens Energy & Environmental Science, Vol. 6, Issue 4 https://doi.org/10.1039/c3ee24403b	journal	January 2013
Lignin to lipid bioconversion by oleaginous Rhodococci Kosa, Matyas; Ragauskas, Arthur J. Green Chemistry, Vol. 15, Issue 8 https://doi.org/10.1039/c3gc40434j	journal	January 2013
Lignin valorization through integrated biological funneling and chemical catalysis Linger, J. G.; Vardon, D. R.; Guarnieri, M. T. Proceedings of the National Academy of Sciences, Vol. 111, Issue 33, p. 12013-12018 https://doi.org/10.1073/pnas.1410657111	journal	August 2014
Biofuels in the long-run global energy supply mix for transportation Timilsina, Govinda R. Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, Vol. 372, Issue 2006 https://doi.org/10.1098/rsta.2012.0323	journal	January 2014
The Path Forward for Biofuels and Biomaterials Ragauskas, Arthur J.; Williams, Charlotte K.; Davison, Brian H. Science, Vol. 311, Issue 5760, p. 484-489 https://doi.org/10.1126/science.1114736	journal	January 2006
Beneficial Biofuels--The Food, Energy, and Environment Trilemma Tilman, D.; Socolow, R.; Foley, J. A. Science, Vol. 325, Issue 5938 https://doi.org/10.1126/science.1177970	journal	July 2009
Lignin Valorization: Improving Lignin Processing in the Biorefinery Ragauskas, A. J.; Beckham, G. T.; Biddy, M. J. Science, Vol. 344, Issue 6185, p. 1246843-1246843 https://doi.org/10.1126/science.1246843	journal	May 2014
Structural characterization of alkaline hydrogen peroxide pretreated grasses exhibiting diverse lignin phenotypes Li, Muyang; Foster, Cliff; Kelkar, Shantanu Biotechnology for Biofuels, Vol. 5, Issue 1 https://doi.org/10.1186/1754-6834-5-38	journal	January 2012
Helically agitated mixing in dry dilute acid pretreatment enhances the bioconversion of corn stover into ethanol He, Yanqing; Zhang, Longping; Zhang, Jian Biotechnology for Biofuels, Vol. 7, Issue 1 https://doi.org/10.1186/1754-6834-7-1	journal	January 2014
Lipid production from corn stover by the oleaginous yeast Cryptococcus curvatus Gong, Zhiwei; Shen, Hongwei; Yang, Xiaobing Biotechnology for Biofuels, Vol. 7, Issue 1 https://doi.org/10.1186/s13068-014-0158-y	journal	October 2014
Microalgal lipid production using the hydrolysates of rice straw pretreated with gamma irradiation and alkali solution Joe, Min-Ho; Kim, Ji-Youn; Lim, Sangyong Biotechnology for Biofuels, Vol. 8, Issue 1 https://doi.org/10.1186/s13068-015-0308-x	journal	August 2015

Cited By (10)

Biotransformation of lignin: Mechanisms, applications and future work Li, Xiang; Zheng, Yi Biotechnology Progress, Vol. 36, Issue 1 https://doi.org/10.1002/btpr.2922	journal	October 2019
Synergistic maximization of the carbohydrate output and lignin processability by combinatorial pretreatment Liu, Zhi-Hua; Olson, Michelle L.; Shinde, Somnath Green Chem., Vol. 19, Issue 20 https://doi.org/10.1039/c7gc02057k	journal	January 2017
Integrated diesel production from lignocellulosic sugars via oleaginous yeast Sànchez i. Nogué, Violeta; Black, Brenna A.; Kruger, Jacob S. Green Chemistry, Vol. 20, Issue 18 https://doi.org/10.1039/c8gc01905c	journal	January 2018
Combinatorial pretreatment and fermentation optimization enabled a record yield on lignin bioconversion Liu, Zhi-Hua; Xie, Shangxian; Lin, Furong Biotechnology for Biofuels, Vol. 11, Issue 1 https://doi.org/10.1186/s13068-018-1021-3	journal	January 2018
RETRACTED ARTICLE: Bacterial conversion of depolymerized Kraft lignin Ravi, Krithika; Abdelaziz, Omar Y.; Nöbel, Matthias Biotechnology for Biofuels, Vol. 11, Issue 1 https://doi.org/10.1186/s13068-018-1240-7	journal	September 2018
Lipid metabolism of phenol-tolerant Rhodococcus opacus strains for lignin bioconversion Henson, William R.; Hsu, Fong-Fu; Dantas, Gautam Biotechnology for Biofuels, Vol. 11, Issue 1 https://doi.org/10.1186/s13068-018-1337-z	journal	December 2018
Recent advances in lignin valorization with bacterial cultures: microorganisms, metabolic pathways, and bio-products Xu, Zhaoxian; Lei, Peng; Zhai, Rui Biotechnology for Biofuels, Vol. 12, Issue 1 https://doi.org/10.1186/s13068-019-1376-0	journal	February 2019
Bacterial conversion of depolymerized Kraft lignin Ravi, Krithika; Abdelaziz, Omar Y.; Nöbel, Matthias Biotechnology for Biofuels, Vol. 12, Issue 1 https://doi.org/10.1186/s13068-019-1397-8	journal	March 2019
Development of Rhodococcus opacus as a chassis for lignin valorization and bioproduction of high-value compounds Anthony, Winston E.; Carr, Rhiannon R.; DeLorenzo, Drew M. Biotechnology for Biofuels, Vol. 12, Issue 1 https://doi.org/10.1186/s13068-019-1535-3	journal	August 2019
Utilization of simultaneous saccharification and fermentation residues as feedstock for lipid accumulation in Rhodococcus opacus Le, Rosemary K.; Das, Parthapratim; Mahan, Kristina M. AMB Express, Vol. 7, Issue 1 https://doi.org/10.1186/s13568-017-0484-0	journal	September 2017

Similar Records

Microbial lipid production by oleaginous Rhodococci cultured in lignocellulosic autohydrolysates

Journal Article · 2015 · Applied Microbiology and Biotechnology · OSTI ID:1399965

Wei, Zhen; Zeng, Guangming; Huang, Fang; +5 more

Bioconversion of lignocellulosic pretreatment effluent via oleaginous Rhodococcus opacus DSM 1069

Journal Article · 2014 · Biomass and Bioenergy · OSTI ID:1399955

Wells, Jr., Tyrone; Wei, Zhen; Ragauskas, Arthur J.

Pyrolysis Oil-Based Lipid Production as Biodiesel Feedstock by Rhodococcus opacus

Journal Article · 2014 · Applied Biochemistry and Biotechnology · OSTI ID:1399961

Wei, Zhen; Zeng, Guangming; Kosa, Matyas; +2 more

Related Subjects

09 BIOMASS FUELS
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES

Title: Conversion of corn stover alkaline pre-treatment waste streams into biodiesel via Rhodococci

Citation Formats

References (50)

Cited By (10)

Similar Records

Related Subjects