Pyrolysis Oil-Based Lipid Production as Biodiesel Feedstock by Rhodococcus opacus

Wei, Zhen; Zeng, Guangming; Kosa, Matyas; Huang, Danlian; Ragauskas, Arthur J.

doi:10.1007/s12010-014-1305-4

Pyrolysis Oil-Based Lipid Production as Biodiesel Feedstock by Rhodococcus opacus

Journal Article · Thu Nov 06 23:00:00 EST 2014 · Applied Biochemistry and Biotechnology

DOI:https://doi.org/10.1007/s12010-014-1305-4· OSTI ID:1399961

Wei, Zhen; Zeng, Guangming; Kosa, Matyas; Huang, Danlian; Ragauskas, Arthur J.

Light oil from pyrolysis, which accounts for ~10 % carbon yield of the starting biomass, is a complex aqueous product that is difficult to utilize and usually discarded. This work presents the feasibility of light oil as a sole carbon source to support the growth of Rhodococcus opacus (R. opacus) that in turn accumulate triacylglycerols as biodiesel feedstock. Two types of bacteria (R. opacus PD630 and DSM 1069) were selected in this study. Research results showed that after short adaption periods both strains can grow well on this complex carbon source, as proved by the consumption of oligomers and monomers in light oil. Lipid content by R. opacus PD630 and DSM 1069 was observed up to 25.8 % and 22.0 % of cell dry weight, respectively. Palmitic and stearic acids were found to be the predominant fatty acids in these bacterial cells. In addition, the light oil-based lipid production can be enhanced by reducing the pH value from 7 to 4, especially in case of DSM 1069.

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-00OR22725

OSTI ID:: 1399961

Journal Information:: Applied Biochemistry and Biotechnology, Journal Name: Applied Biochemistry and Biotechnology Journal Issue: 2 Vol. 175; ISSN 0273-2289

Country of Publication:: United States

Language:: English

References (26)

High cell density cultivation of Rhodococcus opacus for lipid production at a pilot-plant scale Voss, I.; Steinbüchel, A. Applied Microbiology and Biotechnology, Vol. 55, Issue 5 https://doi.org/10.1007/s002530000576	journal	May 2001
The β-Ketoadipate Pathway And The Biology Of Self-Identity Harwood, Caroline S.; Parales, Rebecca E. Annual Review of Microbiology, Vol. 50, Issue 1, p. 553-590 https://doi.org/10.1146/annurev.micro.50.1.553	journal	October 1996
Fuel properties and nitrogen oxide emission levels of biodiesel produced from animal fats Wyatt, Victor T.; Hess, Melissa A.; Dunn, Robert O. Journal of the American Oil Chemists' Society, Vol. 82, Issue 8 https://doi.org/10.1007/s11746-005-1113-2	journal	August 2005
Switchgrass as an energy crop for biofuel production: A review of its ligno-cellulosic chemical properties David, Kasi; Ragauskas, Arthur J. Energy & Environmental Science, Vol. 3, Issue 9 https://doi.org/10.1039/b926617h	journal	January 2010
Biotechnological opportunities with the β-ketoadipate pathway Wells, Tyrone; Ragauskas, Arthur J. Trends in Biotechnology, Vol. 30, Issue 12 https://doi.org/10.1016/j.tibtech.2012.09.008	journal	December 2012
Novozym 435-catalysed transesterification of crude soya bean oils for biodiesel production in a solvent-free medium Du, Wei; Xu, Yuan-yuan; Zeng, Jing Biotechnology and Applied Biochemistry, Vol. 40, Issue 2 https://doi.org/10.1042/BA20030142	journal	October 2004
Bioconversion of lignin model compounds with oleaginous Rhodococci Kosa, Matyas; Ragauskas, Arthur J. Applied Microbiology and Biotechnology, Vol. 93, Issue 2 https://doi.org/10.1007/s00253-011-3743-z	journal	December 2011
Biodiesel production by microalgal biotechnology Huang, GuanHua; Chen, Feng; Wei, Dong Applied Energy, Vol. 87, Issue 1 https://doi.org/10.1016/j.apenergy.2009.06.016	journal	January 2010
Enzymatic cleavage of aromatic rings: mechanistic aspects of the catechol dioxygenases and later enzymes of bacterial oxidative cleavage pathways D. H. Bugg, Timothy; J. Winfield, Christopher Natural Product Reports, Vol. 15, Issue 5 https://doi.org/10.1039/a815513y	journal	January 1998
Renewable Chemical Commodity Feedstocks from Integrated Catalytic Processing of Pyrolysis Oils Vispute, T. P.; Zhang, H.; Sanna, A. Science, Vol. 330, Issue 6008 https://doi.org/10.1126/science.1194218	journal	November 2010
Efficient lipid production with Trichosporonfermentans and its use for biodiesel preparation Zhu, L. Y.; Zong, M. H.; Wu, H. Bioresource Technology, Vol. 99, Issue 16 https://doi.org/10.1016/j.biortech.2008.02.033	journal	November 2008
Lipids from heterotrophic microbes: advances in metabolism research Kosa, Matyas; Ragauskas, Arthur J. Trends in Biotechnology, Vol. 29, Issue 2 https://doi.org/10.1016/j.tibtech.2010.11.002	journal	February 2011
Accumulation and mobilization of storage lipids by Rhodococcus opacus PD630 and Rhodococcus ruber NCIMB 40126 Alvarez, H. M.; Kalscheuer, R.; Steinbüchel, A. Applied Microbiology and Biotechnology, Vol. 54, Issue 2 https://doi.org/10.1007/s002530000395	journal	August 2000
Single cell oil production by Gordonia sp. DG using agro-industrial wastes Gouda, Mona K.; Omar, Sanaa H.; Aouad, Linda M. World Journal of Microbiology and Biotechnology, Vol. 24, Issue 9 https://doi.org/10.1007/s11274-008-9664-z	journal	January 2008
Degradation of coniferyl alcohol and other lignin-related aromatic compounds by Nocardia sp. DSM 1069 Eggeling, L.; Sahm, H. Archives of Microbiology, Vol. 126, Issue 2 https://doi.org/10.1007/BF00511219	journal	June 1980
Triacylglycerols in prokaryotic microorganisms H., Alvarez; A., Steinbüchel Applied Microbiology and Biotechnology, Vol. 60, Issue 4 https://doi.org/10.1007/s00253-002-1135-0	journal	December 2002
Microbial oil production from rice straw hydrolysate by Trichosporon fermentans Huang, Chao; Zong, Min-hua; Wu, Hong Bioresource Technology, Vol. 100, Issue 19 https://doi.org/10.1016/j.biortech.2009.04.022	journal	October 2009
Upgrading of liquid fuel from the pyrolysis of biomass Zhang, S.; Yan, Y.; Li, T. Bioresource Technology, Vol. 96, Issue 5 https://doi.org/10.1016/j.biortech.2004.06.015	journal	March 2005
Cyanobacteria cultivation in industrial wastewaters and biodiesel production from their biomass: A review Balasubramanian, Lavanya; Subramanian, Geetha; Nazeer, Thayiba Thanveer Biotechnology and Applied Biochemistry, Vol. 58, Issue 4 https://doi.org/10.1002/bab.31	journal	July 2011
Dependence of biodiesel fuel properties on the structure of fatty acid alkyl esters Knothe, Gerhard Fuel Processing Technology, Vol. 86, Issue 10 https://doi.org/10.1016/j.fuproc.2004.11.002	journal	June 2005
Cultivation, photobioreactor design and harvesting of microalgae for biodiesel production: A critical review Chen, Chun-Yen; Yeh, Kuei-Ling; Aisyah, Rifka Bioresource Technology, Vol. 102, Issue 1 https://doi.org/10.1016/j.biortech.2010.06.159	journal	January 2011
Production of renewable gasoline from aqueous phase hydrogenation of lignin pyrolysis oil Ben, Haoxi; Mu, Wei; Deng, Yulin Fuel, Vol. 103 https://doi.org/10.1016/j.fuel.2012.08.039	journal	January 2013
Formation of intracytoplasmic lipid inclusions by Rhodococcus opacus strain PD630 Alvarez, Hector M.; Mayer, Frank; Fabritius, Dirk Archives of Microbiology, Vol. 165, Issue 6 https://doi.org/10.1007/s002030050341	journal	June 1996
NMR Characterization of Pyrolysis Oils from Kraft Lignin Ben, Haoxi; Ragauskas, Arthur J. Energy & Fuels, Vol. 25, Issue 5 https://doi.org/10.1021/ef2001162	journal	May 2011
Production of hydrogen, alkanes and polyols by aqueous phase processing of wood-derived pyrolysis oils Vispute, Tushar P.; Huber, George W. Green Chemistry, Vol. 11, Issue 9 https://doi.org/10.1039/b912522c	journal	January 2009
Phosphorus-31 NMR spectroscopic analysis of coal pyrolysis condensates and extracts for heteroatom functionalities possessing labile hydrogen Wroblewski, A. E.; Lensink, C.; Markuszewski, R. Energy & Fuels, Vol. 2, Issue 6 https://doi.org/10.1021/ef00012a008	journal	November 1988

Similar Records

Bioconversion of lignocellulosic pretreatment effluent via oleaginous Rhodococcus opacus DSM 1069

Journal Article · Tue Nov 25 23:00:00 EST 2014 · Biomass and Bioenergy · OSTI ID:1399955

Related Subjects

09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES

Pyrolysis Oil-Based Lipid Production as Biodiesel Feedstock by Rhodococcus opacus

Citation Formats

References (26)

Similar Records

Related Subjects