Overexpression of a Domain of Unknown Function 266-containing protein results in high cellulose content, reduced recalcitrance, and enhanced plant growth in the bioenergy crop Populus
Abstract
Domain of Unknown Function 266 (DUF266) is a plant-specific domain. DUF266-containing proteins (DUF266 proteins) have been categorized as ‘not classified glycosyltransferases (GTnc)’ due to amino acid similarity with GTs. However, little is known about the function of DUF266 proteins. As a result, Phylogenetic analysis revealed that DUF266 proteins are only present in the land plants including moss and lycophyte. We report the functional characterization of one member of DUF266 proteins in Populus, PdDUF266A. PdDUF266A was ubiquitously expressed with high abundance in the xylem. In Populus transgenic plants overexpressing PdDUF266A (OXPdDUF266A), the glucose and cellulose contents were significantly higher, while the lignin content was lower than that in the wild type. Degree of polymerization of cellulose in OXPdDUF266A transgenic plants was also higher, whereas cellulose crystallinity index remained unchanged. Gene expression analysis indicated that cellulose biosynthesis-related genes such as CESA and SUSY were upregulated in mature leaf and xylem of OXPdDUF266A transgenic plants. Moreover, PdDUF266A overexpression resulted in an increase of biomass production. Their glucose contents and biomass phenotypes were further validated via heterologous expression of PdDUF266A in Arabidopsis. Results from saccharification treatment demonstrated that the rate of sugar release was increased by approximately 38% in the OXPdDUF266A transgenic plants.
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Joint Institute for Biological Sciences (JIBS)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1618674
- Alternate Identifier(s):
- OSTI ID: 1356900
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Published Article
- Journal Name:
- Biotechnology for Biofuels
- Additional Journal Information:
- Journal Name: Biotechnology for Biofuels Journal Volume: 10 Journal Issue: 1; Journal ID: ISSN 1754-6834
- Publisher:
- Springer Science + Business Media
- Country of Publication:
- Netherlands
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; 60 APPLIED LIFE SCIENCES; DUF266; recalcitrance; Populus; bioenergy; biofuel; biomass; cell wall; cellulose; sugar release
Citation Formats
Yang, Yongil, Yoo, Chang Geun, Guo, Hao-Bo, Rottmann, William, Winkeler, Kimberly A., Collins, Cassandra M., Gunter, Lee E., Jawdy, Sara S., Yang, Xiaohan, Guo, Hong, Pu, Yunqiao, Ragauskas, Arthur J., Tuskan, Gerald A., and Chen, Jin-Gui. Overexpression of a Domain of Unknown Function 266-containing protein results in high cellulose content, reduced recalcitrance, and enhanced plant growth in the bioenergy crop Populus. Netherlands: N. p., 2017.
Web. doi:10.1186/s13068-017-0760-x.
Yang, Yongil, Yoo, Chang Geun, Guo, Hao-Bo, Rottmann, William, Winkeler, Kimberly A., Collins, Cassandra M., Gunter, Lee E., Jawdy, Sara S., Yang, Xiaohan, Guo, Hong, Pu, Yunqiao, Ragauskas, Arthur J., Tuskan, Gerald A., & Chen, Jin-Gui. Overexpression of a Domain of Unknown Function 266-containing protein results in high cellulose content, reduced recalcitrance, and enhanced plant growth in the bioenergy crop Populus. Netherlands. https://doi.org/10.1186/s13068-017-0760-x
Yang, Yongil, Yoo, Chang Geun, Guo, Hao-Bo, Rottmann, William, Winkeler, Kimberly A., Collins, Cassandra M., Gunter, Lee E., Jawdy, Sara S., Yang, Xiaohan, Guo, Hong, Pu, Yunqiao, Ragauskas, Arthur J., Tuskan, Gerald A., and Chen, Jin-Gui. Thu .
"Overexpression of a Domain of Unknown Function 266-containing protein results in high cellulose content, reduced recalcitrance, and enhanced plant growth in the bioenergy crop Populus". Netherlands. https://doi.org/10.1186/s13068-017-0760-x.
@article{osti_1618674,
title = {Overexpression of a Domain of Unknown Function 266-containing protein results in high cellulose content, reduced recalcitrance, and enhanced plant growth in the bioenergy crop Populus},
author = {Yang, Yongil and Yoo, Chang Geun and Guo, Hao-Bo and Rottmann, William and Winkeler, Kimberly A. and Collins, Cassandra M. and Gunter, Lee E. and Jawdy, Sara S. and Yang, Xiaohan and Guo, Hong and Pu, Yunqiao and Ragauskas, Arthur J. and Tuskan, Gerald A. and Chen, Jin-Gui},
abstractNote = {Domain of Unknown Function 266 (DUF266) is a plant-specific domain. DUF266-containing proteins (DUF266 proteins) have been categorized as ‘not classified glycosyltransferases (GTnc)’ due to amino acid similarity with GTs. However, little is known about the function of DUF266 proteins. As a result, Phylogenetic analysis revealed that DUF266 proteins are only present in the land plants including moss and lycophyte. We report the functional characterization of one member of DUF266 proteins in Populus, PdDUF266A. PdDUF266A was ubiquitously expressed with high abundance in the xylem. In Populus transgenic plants overexpressing PdDUF266A (OXPdDUF266A), the glucose and cellulose contents were significantly higher, while the lignin content was lower than that in the wild type. Degree of polymerization of cellulose in OXPdDUF266A transgenic plants was also higher, whereas cellulose crystallinity index remained unchanged. Gene expression analysis indicated that cellulose biosynthesis-related genes such as CESA and SUSY were upregulated in mature leaf and xylem of OXPdDUF266A transgenic plants. Moreover, PdDUF266A overexpression resulted in an increase of biomass production. Their glucose contents and biomass phenotypes were further validated via heterologous expression of PdDUF266A in Arabidopsis. Results from saccharification treatment demonstrated that the rate of sugar release was increased by approximately 38% in the OXPdDUF266A transgenic plants.},
doi = {10.1186/s13068-017-0760-x},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 10,
place = {Netherlands},
year = {Thu Mar 23 00:00:00 EDT 2017},
month = {Thu Mar 23 00:00:00 EDT 2017}
}
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https://doi.org/10.1186/s13068-017-0760-x
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Works referenced in this record:
Molecular biology of the plant cell wall: searching for the genes that define structure, architecture and dynamics
journal, September 2001
- Carpita, Nick; Tierney, Mary; Campbell, Malcolm
- Plant Molecular Biology, Vol. 47, Issue 1/2, p. 1-5
Pectin Biosynthesis: GALS1 in Arabidopsis thaliana Is a β-1,4-Galactan β-1,4-Galactosyltransferase
journal, December 2012
- Liwanag, April Jennifer Madrid; Ebert, Berit; Verhertbruggen, Yves
- The Plant Cell, Vol. 24, Issue 12
Arabidopsis thaliana T-DNA Mutants Implicate GAUT Genes in the Biosynthesis of Pectin and Xylan in Cell Walls and Seed Testa
journal, September 2009
- Caffall, Kerry H.; Pattathil, Sivakumar; Phillips, Sarah E.
- Molecular Plant, Vol. 2, Issue 5
Improved Gateway Binary Vectors: High-Performance Vectors for Creation of Fusion Constructs in Transgenic Analysis of Plants
journal, August 2007
- Nakagawa, Tsuyoshi; Suzuki, Takamasa; Murata, Satoko
- Bioscience, Biotechnology, and Biochemistry, Vol. 71, Issue 8
Hemicelluloses
journal, June 2010
- Scheller, Henrik Vibe; Ulvskov, Peter
- Annual Review of Plant Biology, Vol. 61, Issue 1
Xylan biosynthesis
journal, April 2014
- Rennie, Emilie A.; Scheller, Henrik Vibe
- Current Opinion in Biotechnology, Vol. 26
The Pfam protein families database: towards a more sustainable future
journal, December 2015
- Finn, Robert D.; Coggill, Penelope; Eberhardt, Ruth Y.
- Nucleic Acids Research, Vol. 44, Issue D1
Effect of acid-chlorite delignification on cellulose degree of polymerization
journal, October 2010
- Hubbell, Christopher A.; Ragauskas, Arthur J.
- Bioresource Technology, Vol. 101, Issue 19
Cellulose Synthases and Synthesis in Arabidopsis
journal, March 2011
- Endler, Anne; Persson, Staffan
- Molecular Plant, Vol. 4, Issue 2
MoRFpred, a computational tool for sequence-based prediction and characterization of short disorder-to-order transitioning binding regions in proteins
journal, June 2012
- Disfani, Fatemeh Miri; Hsu, Wei-Lun; Mizianty, Marcin J.
- Bioinformatics, Vol. 28, Issue 12
Galacturonosyltransferase (GAUT)1 and GAUT7 are the core of a plant cell wall pectin biosynthetic homogalacturonan:galacturonosyltransferase complex
journal, November 2011
- Atmodjo, M. A.; Sakuragi, Y.; Zhu, X.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 50
The plant glycosyltransferase clone collection for functional genomics
journal, July 2014
- Lao, Jeemeng; Oikawa, Ai; Bromley, Jennifer R.
- The Plant Journal, Vol. 79, Issue 3
I-TASSER: a unified platform for automated protein structure and function prediction
journal, March 2010
- Roy, Ambrish; Kucukural, Alper; Zhang, Yang
- Nature Protocols, Vol. 5, Issue 4
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
journal, January 1994
- Thompson, Julie D.; Higgins, Desmond G.; Gibson, Toby J.
- Nucleic Acids Research, Vol. 22, Issue 22, p. 4673-4680
De-DUFing the DUFs: Deciphering distant evolutionary relationships of Domains of Unknown Function using sensitive homology detection methods
journal, July 2015
- Mudgal, Richa; Sandhya, Sankaran; Chandra, Nagasuma
- Biology Direct, Vol. 10, Issue 1
A β-glucuronosyltransferase from Arabidopsis thaliana involved in biosynthesis of type II arabinogalactan has a role in cell elongation during seedling growth
journal, November 2013
- Knoch, Eva; Dilokpimol, Adiphol; Tryfona, Theodora
- The Plant Journal, Vol. 76, Issue 6
Assay and heterologous expression in Pichia pastoris of plant cell wall type-II membrane anchored glycosyltransferases
journal, May 2009
- Petersen, Bent Larsen; Egelund, Jack; Damager, Iben
- Glycoconjugate Journal, Vol. 26, Issue 9
Initial characterization of shade avoidance response suggests functional diversity between Populus phytochrome B genes
journal, September 2012
- Karve, Abhijit A.; Jawdy, Sara S.; Gunter, Lee E.
- New Phytologist, Vol. 196, Issue 3
Comparative analysis of GT14/GT14-like gene family in Arabidopsis, Oryza, Populus, Sorghum and Vitis
journal, December 2011
- Ye, Chu-Yu; Li, Ting; Tuskan, Gerald A.
- Plant Science, Vol. 181, Issue 6
Predicting transmembrane protein topology with a hidden markov model: application to complete genomes11Edited by F. Cohen
journal, January 2001
- Krogh, Anders; Larsson, Björn; von Heijne, Gunnar
- Journal of Molecular Biology, Vol. 305, Issue 3
MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets
journal, March 2016
- Kumar, Sudhir; Stecher, Glen; Tamura, Koichiro
- Molecular Biology and Evolution, Vol. 33, Issue 7
Evolving Views of Pectin Biosynthesis
journal, April 2013
- Atmodjo, Melani A.; Hao, Zhangying; Mohnen, Debra
- Annual Review of Plant Biology, Vol. 64, Issue 1
Identification of a Xylogalacturonan Xylosyltransferase Involved in Pectin Biosynthesis in Arabidopsis
journal, May 2008
- Jensen, J. K.; Sorensen, S. O.; Harholt, J.
- The Plant Cell, Vol. 20, Issue 5, p. 1289-1302
Plant Glycosyltransferases Beyond CAZy: A Perspective on DUF Families
journal, January 2012
- Hansen, Sara Fasmer; Harholt, Jesper; Oikawa, Ai
- Frontiers in Plant Science, Vol. 3
Cellulose Synthesis and Its Regulation
journal, January 2014
- Li, Shundai; Bashline, Logan; Lei, Lei
- The Arabidopsis Book, Vol. 12
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana
journal, December 1998
- Clough, Steven J.; Bent, Andrew F.
- The Plant Journal, Vol. 16, Issue 6, p. 735-743
Exploring genomes for glycosyltransferases
journal, January 2010
- Hansen, Sara Fasmer; Bettler, Emmanuel; Rinnan, Åsmund
- Molecular BioSystems, Vol. 6, Issue 10
The Glycosyltransferase Repertoire of the Spikemoss Selaginella moellendorffii and a Comparative Study of Its Cell Wall
journal, May 2012
- Harholt, Jesper; Sørensen, Iben; Fangel, Jonatan
- PLoS ONE, Vol. 7, Issue 5
Arabidopsis thaliana RGXT1 and RGXT2 Encode Golgi-Localized (1,3)-α-d-Xylosyltransferases Involved in the Synthesis of Pectic Rhamnogalacturonan-II
journal, October 2006
- Egelund, Jack; Petersen, Bent Larsen; Motawia, Mohammed Saddik
- The Plant Cell, Vol. 18, Issue 10
Phytozome: a comparative platform for green plant genomics
journal, November 2011
- Goodstein, David M.; Shu, Shengqiang; Howson, Russell
- Nucleic Acids Research, Vol. 40, Issue D1
Storage of competent cells for Agrobacterium transformation
journal, January 1988
- Hofgen, Rainer; Willmitzer, Lothar
- Nucleic Acids Research, Vol. 16, Issue 20
The Carbohydrate-Active EnZymes database (CAZy): an expert resource for Glycogenomics
journal, January 2009
- Cantarel, B. L.; Coutinho, P. M.; Rancurel, C.
- Nucleic Acids Research, Vol. 37, Issue Database
MUSCLE: multiple sequence alignment with high accuracy and high throughput
journal, March 2004
- Edgar, R. C.
- Nucleic Acids Research, Vol. 32, Issue 5, p. 1792-1797
Metabolic profiling reveals altered sugar and secondary metabolism in response to UGPase overexpression in Populus
journal, October 2014
- Payyavula, Raja S.; Tschaplinski, Timothy J.; Jawdy, Sara S.
- BMC Plant Biology, Vol. 14, Issue 1
Molecular Cloning and Expression of a Novel β-1,6- N -Acetylglucosaminyltransferase That Forms Core 2, Core 4, and I Branches
journal, January 1999
- Yeh, Jiunn-Chern; Ong, Edgar; Fukuda, Minoru
- Journal of Biological Chemistry, Vol. 274, Issue 5
ARABINAN DEFICIENT 1 Is a Putative Arabinosyltransferase Involved in Biosynthesis of Pectic Arabinan in Arabidopsis
journal, December 2005
- Harholt, Jesper; Jensen, Jacob Krüger; Sørensen, Susanne Oxenbøll
- Plant Physiology, Vol. 140, Issue 1
Semimicro determination of cellulose inbiological materials
journal, December 1969
- Updegraff, David M.
- Analytical Biochemistry, Vol. 32, Issue 3
ARAD proteins associated with pectic Arabinan biosynthesis form complexes when transiently overexpressed in planta
journal, January 2012
- Harholt, Jesper; Jensen, Jacob Krüger; Verhertbruggen, Yves
- Planta, Vol. 236, Issue 1, p. 115-128
Cellulose Isolation Methodology for NMR Analysis of Cellulose Ultrastructure
journal, November 2011
- Foston, Marcus B.; Hubbell, Chistopher A.; Ragauskas, Art J.
- Materials, Vol. 4, Issue 11
Effect of structural and physico-chemical features of cellulosic substrates on the efficiency of enzymatic hydrolysis
journal, July 1991
- Sinitsyn, A. P.; Gusakov, A. V.; Vlasenko, E. Yu.
- Applied Biochemistry and Biotechnology, Vol. 30, Issue 1
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
journal, December 2001
- Livak, Kenneth J.; Schmittgen, Thomas D.
- Methods, Vol. 25, Issue 4
Structures and mechanisms of glycosyltransferases
journal, July 2005
- Breton, Christelle; Šnajdrová, Lenka; Jeanneau, Charlotte
- Glycobiology, Vol. 16, Issue 2
Works referencing / citing this record:
PdWND3A, a wood-associated NAC domain-containing protein, affects lignin biosynthesis and composition in Populus
journal, November 2019
- Yang, Yongil; Yoo, Chang Geun; Rottmann, William
- BMC Plant Biology, Vol. 19, Issue 1
Agronomic performance of Populus deltoides trees engineered for biofuel production
journal, November 2017
- Macaya-Sanz, David; Chen, Jin‐Gui; Kalluri, Udaya C.
- Biotechnology for Biofuels, Vol. 10, Issue 1
A 22-bp deletion in OsPLS3 gene encoding a DUF266-containing protein is implicated in rice leaf senescence
journal, August 2018
- Li, Kunyu; Chen, Yaodong; Luo, Yanmin
- Plant Molecular Biology, Vol. 98, Issue 1-2
Mutation in a putative glycosyltransferase-like gene causes programmed cell death and early leaf senescence in rice
journal, February 2019
- Ke, Shanwen; Liu, Shuchun; Luan, Xin
- Rice, Vol. 12, Issue 1
Overexpression of a Domain of Unknown Function 231-containing protein increases O-xylan acetylation and cellulose biosynthesis in Populus
journal, December 2017
- Yang, Yongil; Yoo, Chang Geun; Winkeler, Kimberly A.
- Biotechnology for Biofuels, Vol. 10, Issue 1