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Title: Overexpression of a serine hydroxymethyltransferase increases biomass production and reduces recalcitrance in the bioenergy crop Populus

Abstract

Cell wall recalcitrance is the major obstacle for plant biomass conversion to biofuels. In this study, we functionally characterized a serine hydroxymethyltransferase (SHMT) from Populus and evaluated its potential for developing lignocellulosic feedstocks. SHMT is an enzyme that plays an important role in cellular one-carbon pathways. However, little is known about its function in plant cell wall-related processes. Among nine SHMT genes in the Populus genome, PtSHMT2 was highly expressed in the developing xylem and was co-expressed with secondary cell wall biosynthetic genes. In Populus transgenic plants overexpressing PtSHMT2, the biomass yield and sugar (glucose and xylose) release were increased whereas the lignin content was decreased. Transcriptomics and metabolomics analyses revealed that genes and metabolites related to secondary cell wall biosynthesis were affected by PtSHMT2 overexpression. Based on the transcription factor binding sites of differentially expressed genes in PtSHMT2 overexpression lines, a total of 27 hub transcription factors were identified. We proposed a regulatory model of action of PtSHMT2 with transcriptional master switches of secondary cell wall biosynthesis. As a whole, these results suggest that PtSHMT2 is a promising candidate for genetic engineering to improve feedstock characteristics to enhance biofuel conversion and reduce the cost of lignocellulosic biofuel production.

Authors:
ORCiD logo [1]; ORCiD logo [2];  [2]; ORCiD logo [1];  [3];  [3];  [3]; ORCiD logo [4]; ORCiD logo [4]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [4]; ORCiD logo [1]; ORCiD logo [5]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1] more »; ORCiD logo [1] « less
  1. BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, USA, Center for Bioenergy Innovation
  2. BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, USA
  3. ArborGen Inc., Ridgeville, USA
  4. U.S. Department of Energy Joint Genome Institute, Walnut Creek, USA
  5. U.S. Department of Energy Joint Genome Institute, Walnut Creek, USA, HudsonAlpha Institute for Biotechnology, Huntsville
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1480552
Alternate Identifier(s):
OSTI ID: 1502582
Grant/Contract Number:  
AC05-00OR22725; AC02-05CH11231
Resource Type:
Published Article
Journal Name:
Sustainable Energy & Fuels
Additional Journal Information:
Journal Name: Sustainable Energy & Fuels Journal Volume: 3 Journal Issue: 1; Journal ID: ISSN 2398-4902
Publisher:
Royal Society of Chemistry
Country of Publication:
United Kingdom
Language:
English
Subject:
09 BIOMASS FUELS

Citation Formats

Zhang, Jin, Li, Mi, Bryan, Anthony C., Yoo, Chang Geun, Rottmann, William, Winkeler, Kimberly A., Collins, Cassandra M., Singan, Vasanth, Lindquist, Erika A., Jawdy, Sara S., Gunter, Lee E., Engle, Nancy L., Yang, Xiaohan, Barry, Kerrie, Tschaplinski, Timothy J., Schmutz, Jeremy, Pu, Yunqiao, Ragauskas, Arthur J., Tuskan, Gerald A., Muchero, Wellington, and Chen, Jin-Gui. Overexpression of a serine hydroxymethyltransferase increases biomass production and reduces recalcitrance in the bioenergy crop Populus. United Kingdom: N. p., 2019. Web. doi:10.1039/C8SE00471D.
Zhang, Jin, Li, Mi, Bryan, Anthony C., Yoo, Chang Geun, Rottmann, William, Winkeler, Kimberly A., Collins, Cassandra M., Singan, Vasanth, Lindquist, Erika A., Jawdy, Sara S., Gunter, Lee E., Engle, Nancy L., Yang, Xiaohan, Barry, Kerrie, Tschaplinski, Timothy J., Schmutz, Jeremy, Pu, Yunqiao, Ragauskas, Arthur J., Tuskan, Gerald A., Muchero, Wellington, & Chen, Jin-Gui. Overexpression of a serine hydroxymethyltransferase increases biomass production and reduces recalcitrance in the bioenergy crop Populus. United Kingdom. doi:10.1039/C8SE00471D.
Zhang, Jin, Li, Mi, Bryan, Anthony C., Yoo, Chang Geun, Rottmann, William, Winkeler, Kimberly A., Collins, Cassandra M., Singan, Vasanth, Lindquist, Erika A., Jawdy, Sara S., Gunter, Lee E., Engle, Nancy L., Yang, Xiaohan, Barry, Kerrie, Tschaplinski, Timothy J., Schmutz, Jeremy, Pu, Yunqiao, Ragauskas, Arthur J., Tuskan, Gerald A., Muchero, Wellington, and Chen, Jin-Gui. Tue . "Overexpression of a serine hydroxymethyltransferase increases biomass production and reduces recalcitrance in the bioenergy crop Populus". United Kingdom. doi:10.1039/C8SE00471D.
@article{osti_1480552,
title = {Overexpression of a serine hydroxymethyltransferase increases biomass production and reduces recalcitrance in the bioenergy crop Populus},
author = {Zhang, Jin and Li, Mi and Bryan, Anthony C. and Yoo, Chang Geun and Rottmann, William and Winkeler, Kimberly A. and Collins, Cassandra M. and Singan, Vasanth and Lindquist, Erika A. and Jawdy, Sara S. and Gunter, Lee E. and Engle, Nancy L. and Yang, Xiaohan and Barry, Kerrie and Tschaplinski, Timothy J. and Schmutz, Jeremy and Pu, Yunqiao and Ragauskas, Arthur J. and Tuskan, Gerald A. and Muchero, Wellington and Chen, Jin-Gui},
abstractNote = {Cell wall recalcitrance is the major obstacle for plant biomass conversion to biofuels. In this study, we functionally characterized a serine hydroxymethyltransferase (SHMT) from Populus and evaluated its potential for developing lignocellulosic feedstocks. SHMT is an enzyme that plays an important role in cellular one-carbon pathways. However, little is known about its function in plant cell wall-related processes. Among nine SHMT genes in the Populus genome, PtSHMT2 was highly expressed in the developing xylem and was co-expressed with secondary cell wall biosynthetic genes. In Populus transgenic plants overexpressing PtSHMT2, the biomass yield and sugar (glucose and xylose) release were increased whereas the lignin content was decreased. Transcriptomics and metabolomics analyses revealed that genes and metabolites related to secondary cell wall biosynthesis were affected by PtSHMT2 overexpression. Based on the transcription factor binding sites of differentially expressed genes in PtSHMT2 overexpression lines, a total of 27 hub transcription factors were identified. We proposed a regulatory model of action of PtSHMT2 with transcriptional master switches of secondary cell wall biosynthesis. As a whole, these results suggest that PtSHMT2 is a promising candidate for genetic engineering to improve feedstock characteristics to enhance biofuel conversion and reduce the cost of lignocellulosic biofuel production.},
doi = {10.1039/C8SE00471D},
journal = {Sustainable Energy & Fuels},
number = 1,
volume = 3,
place = {United Kingdom},
year = {2019},
month = {1}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1039/C8SE00471D

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Cited by: 5 works
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