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Title: Overexpression of a Prefoldin β subunit gene reduces biomass recalcitrance in the bioenergy crop Populus

Abstract

Prefoldin (PFD) is a group II chaperonin that is ubiquitously present in the eukaryotic kingdom. Six subunits (PFD1-6) form a jellyfish-like heterohexameric PFD complex and function in protein folding and cytoskeleton organization. However, little is known about its function in plant cell wall-related processes. Here, we report the functional characterization of a PFD gene from em>Populus deltoides, designated as PdPFD2.2 There are two copies of PFD2 in em>Populus and PdPFD2.2 was ubiquitously expressed with high transcript abundance in the cambial region. PdPFD2.2 can physically interact with DELLA protein RGA1_8g, and its subcellular localization is affected by the interaction. In P. deltoides transgenic plants overexpressing PdPFD2.2, the lignin syringyl/guaiacyl ratio was increased, but cellulose content and crystallinity index were unchanged. In addition, the total released sugar (glucose and xylose) amounts were increased by 7.6% and 6.1%, respectively, in two transgenic lines. Transcriptomic and metabolomic analyses revealed that secondary metabolic pathways, including lignin and flavonoid biosynthesis, were affected by overexpressing PdPFD2.2. A total of eight hub transcription factors (TFs) were identified based on TF binding sites of differentially expressed genes in Populus transgenic plants overexpressing PdPFD2.2. In addition, several known cell wall-related TFs, such as MYB3, MYB4, MYB7, TT8, and XND1, weremore » affected by overexpression of PdPFD2.2. These results suggest that overexpression of PdPFD2.2 can reduce biomass recalcitrance and PdPFD2.2 is a promising target for genetic engineering to improve feedstock characteristics to enhance biofuel conversion and reduce the cost of lignocellulosic biofuel production.« less

Authors:
ORCiD logo [1];  [2];  [3];  [4];  [1]; ORCiD logo [5];  [6];  [6];  [6];  [7];  [7];  [1];  [1];  [1];  [1];  [7];  [1];  [8];  [1];  [1] more »; ORCiD logo [1] « less
  1. Biosciences Division Oak Ridge National Laboratory Oak Ridge TN USA, Center for Bioenergy Innovation Oak Ridge National Laboratory Oak Ridge TN USA
  2. Biosciences Division Oak Ridge National Laboratory Oak Ridge TN USA, Center for Bioenergy Innovation Oak Ridge National Laboratory Oak Ridge TN USA, Department of Plant Sciences University of Tennessee Knoxville TN USA
  3. Chemical &, Biomolecular Engineering University of Tennessee Knoxville TN USA
  4. Chemical &, Biomolecular Engineering University of Tennessee Knoxville TN USA, College of Textiles Donghua University Shanghai China
  5. Biosciences Division Oak Ridge National Laboratory Oak Ridge TN USA
  6. ArborGen Inc. Ridgeville SC USA
  7. U.S. Department of Energy Joint Genome Institute Walnut Creek CA USA
  8. U.S. Department of Energy Joint Genome Institute Walnut Creek CA USA, HudsonAlpha Institute for Biotechnology Huntsville AL USA
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1566924
Alternate Identifier(s):
OSTI ID: 1561676; OSTI ID: 1566925; OSTI ID: 1616121
Grant/Contract Number:  
AC02‐05CH11231; AC05‐00OR22725; AC05-00OR22725; AC02‐05CH1123; AC02-05CH11231
Resource Type:
Published Article
Journal Name:
Plant Biotechnology Journal
Additional Journal Information:
Journal Name: Plant Biotechnology Journal; Journal ID: ISSN 1467-7644
Publisher:
Society for Experimental Biology; Association of Applied Biology
Country of Publication:
United Kingdom
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; Populus; prefoldin; biofuels; cell wall recalcitrance; lignin; S/G ratio; transcriptome; metabolome; cell wall reclcitrance

Citation Formats

Zhang, Jin, Xie, Meng, Li, Mi, Ding, Jinhua, Pu, Yunqiao, Bryan, Anthony C., Rottmann, William, Winkeler, Kimberly A., Collins, Cassandra M., Singan, Vasanth, Lindquist, Erika A., Jawdy, Sara S., Gunter, Lee E., Engle, Nancy L., Yang, Xiaohan, Barry, Kerrie, Tschaplinski, Timothy J., Schmutz, Jeremy, Tuskan, Gerald A., Muchero, Wellington, and Chen, Jin‐Gui. Overexpression of a Prefoldin β subunit gene reduces biomass recalcitrance in the bioenergy crop Populus. United Kingdom: N. p., 2019. Web. doi:10.1111/pbi.13254.
Zhang, Jin, Xie, Meng, Li, Mi, Ding, Jinhua, Pu, Yunqiao, Bryan, Anthony C., Rottmann, William, Winkeler, Kimberly A., Collins, Cassandra M., Singan, Vasanth, Lindquist, Erika A., Jawdy, Sara S., Gunter, Lee E., Engle, Nancy L., Yang, Xiaohan, Barry, Kerrie, Tschaplinski, Timothy J., Schmutz, Jeremy, Tuskan, Gerald A., Muchero, Wellington, & Chen, Jin‐Gui. Overexpression of a Prefoldin β subunit gene reduces biomass recalcitrance in the bioenergy crop Populus. United Kingdom. doi:10.1111/pbi.13254.
Zhang, Jin, Xie, Meng, Li, Mi, Ding, Jinhua, Pu, Yunqiao, Bryan, Anthony C., Rottmann, William, Winkeler, Kimberly A., Collins, Cassandra M., Singan, Vasanth, Lindquist, Erika A., Jawdy, Sara S., Gunter, Lee E., Engle, Nancy L., Yang, Xiaohan, Barry, Kerrie, Tschaplinski, Timothy J., Schmutz, Jeremy, Tuskan, Gerald A., Muchero, Wellington, and Chen, Jin‐Gui. Fri . "Overexpression of a Prefoldin β subunit gene reduces biomass recalcitrance in the bioenergy crop Populus". United Kingdom. doi:10.1111/pbi.13254.
@article{osti_1566924,
title = {Overexpression of a Prefoldin β subunit gene reduces biomass recalcitrance in the bioenergy crop Populus},
author = {Zhang, Jin and Xie, Meng and Li, Mi and Ding, Jinhua and Pu, Yunqiao and Bryan, Anthony C. and Rottmann, William and Winkeler, Kimberly A. and Collins, Cassandra M. and Singan, Vasanth and Lindquist, Erika A. and Jawdy, Sara S. and Gunter, Lee E. and Engle, Nancy L. and Yang, Xiaohan and Barry, Kerrie and Tschaplinski, Timothy J. and Schmutz, Jeremy and Tuskan, Gerald A. and Muchero, Wellington and Chen, Jin‐Gui},
abstractNote = {Prefoldin (PFD) is a group II chaperonin that is ubiquitously present in the eukaryotic kingdom. Six subunits (PFD1-6) form a jellyfish-like heterohexameric PFD complex and function in protein folding and cytoskeleton organization. However, little is known about its function in plant cell wall-related processes. Here, we report the functional characterization of a PFD gene from em>Populus deltoides, designated as PdPFD2.2 There are two copies of PFD2 in em>Populus and PdPFD2.2 was ubiquitously expressed with high transcript abundance in the cambial region. PdPFD2.2 can physically interact with DELLA protein RGA1_8g, and its subcellular localization is affected by the interaction. In P. deltoides transgenic plants overexpressing PdPFD2.2, the lignin syringyl/guaiacyl ratio was increased, but cellulose content and crystallinity index were unchanged. In addition, the total released sugar (glucose and xylose) amounts were increased by 7.6% and 6.1%, respectively, in two transgenic lines. Transcriptomic and metabolomic analyses revealed that secondary metabolic pathways, including lignin and flavonoid biosynthesis, were affected by overexpressing PdPFD2.2. A total of eight hub transcription factors (TFs) were identified based on TF binding sites of differentially expressed genes in Populus transgenic plants overexpressing PdPFD2.2. In addition, several known cell wall-related TFs, such as MYB3, MYB4, MYB7, TT8, and XND1, were affected by overexpression of PdPFD2.2. These results suggest that overexpression of PdPFD2.2 can reduce biomass recalcitrance and PdPFD2.2 is a promising target for genetic engineering to improve feedstock characteristics to enhance biofuel conversion and reduce the cost of lignocellulosic biofuel production.},
doi = {10.1111/pbi.13254},
journal = {Plant Biotechnology Journal},
number = ,
volume = ,
place = {United Kingdom},
year = {2019},
month = {9}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1111/pbi.13254

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Cited by: 3 works
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Figures / Tables:

Figure 1 Figure 1: Phylogenetic analysis and expression patterns of the Populus PFD2 genes. (a)Phylogenetic tree constructed using full-length amino acid sequences of PFD2 from 11 plant species by the neighbor-joining (NJ) method with 1000 bootstrap replicates. (b) Expression patterns of the PtPFD2 genes across various tissues. (c) Expression patterns of themore » PtPFD2 genes during wood formation. The data were retrieved from the AspWood database (http://aspwood.popgenie.org/aspwood-v3.0/), where relative expression is shown for aspen stem samples, which consist primarily of phloem, cambium, expanding xylem, and maturing xylem. (d) Co-expression network of PtPFD2.2.« less

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