skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Overexpression of a Prefoldin β subunit gene reduces biomass recalcitrance in the bioenergy crop Populus

Abstract

Prefoldin (PFD) is a group II chaperonin that is ubiquitously present in the eukaryotic kingdom. Six subunits (PFD1-6) form a jellyfish-like heterohexameric PFD complex and function in protein folding and cytoskeleton organization. However, little is known about its function in plant cell wall-related processes. Here, we report the functional characterization of a PFD gene from em>Populus deltoides, designated as PdPFD2.2 There are two copies of PFD2 in em>Populus and PdPFD2.2 was ubiquitously expressed with high transcript abundance in the cambial region. PdPFD2.2 can physically interact with DELLA protein RGA1_8g, and its subcellular localization is affected by the interaction. In P. deltoides transgenic plants overexpressing PdPFD2.2, the lignin syringyl/guaiacyl ratio was increased, but cellulose content and crystallinity index were unchanged. In addition, the total released sugar (glucose and xylose) amounts were increased by 7.6% and 6.1%, respectively, in two transgenic lines. Transcriptomic and metabolomic analyses revealed that secondary metabolic pathways, including lignin and flavonoid biosynthesis, were affected by overexpressing PdPFD2.2. A total of eight hub transcription factors (TFs) were identified based on TF binding sites of differentially expressed genes in Populus transgenic plants overexpressing PdPFD2.2. In addition, several known cell wall-related TFs, such as MYB3, MYB4, MYB7, TT8, and XND1, weremore » affected by overexpression of PdPFD2.2. These results suggest that overexpression of PdPFD2.2 can reduce biomass recalcitrance and PdPFD2.2 is a promising target for genetic engineering to improve feedstock characteristics to enhance biofuel conversion and reduce the cost of lignocellulosic biofuel production.« less

Authors:
ORCiD logo [1]; ORCiD logo [2];  [3];  [4]; ORCiD logo [1]; ORCiD logo [1];  [5];  [5];  [5];  [6];  [6];  [1]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1];  [6]; ORCiD logo [1];  [7]; ORCiD logo [1]; ORCiD logo [1] more »; ORCiD logo [1] « less
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
  3. Univ. of Tennessee, Knoxville, TN (United States)
  4. Univ. of Tennessee, Knoxville, TN (United States); Donghua Univ., Shanghai (China)
  5. ArborGen Inc., Ridgeville, SC (United States)
  6. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
  7. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); HudsonAlpha Inst. for Biotechnology, Huntsville, AL (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1566924
Alternate Identifier(s):
OSTI ID: 1561676; OSTI ID: 1566925
Grant/Contract Number:  
AC05-00OR22725; AC02‐05CH1123; AC02‐05CH11231; AC05‐00OR22725
Resource Type:
Published Article
Journal Name:
Plant Biotechnology Journal
Additional Journal Information:
Journal Name: Plant Biotechnology Journal; Journal ID: ISSN 1467-7644
Publisher:
Society for Experimental Biology; Association of Applied Biology
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; Populus; prefoldin; biofuels; cell wall recalcitrance; lignin; S/G ratio; transcriptome; metabolome

Citation Formats

Zhang, Jin, Xie, Meng, Li, Mi, Ding, Jinhua, Pu, Yunqiao Joseph, Bryan, Anthony C., Rottmann, William, Winkeler, Kimberly A., Collins, Cassandra M., Singan, Vasanth, Lindquist, Erika A., Jawdy, Sara S., Gunter, Lee E., Engle, Nancy L., Yang, Xiaohan, Barry, Kerrie, Tschaplinski, Timothy J., Schmutz, Jeremy, Tuskan, Gerald A., Muchero, Wellington, and Chen, Jin-Gui. Overexpression of a Prefoldin β subunit gene reduces biomass recalcitrance in the bioenergy crop Populus. United States: N. p., 2019. Web. doi:10.1111/pbi.13254.
Zhang, Jin, Xie, Meng, Li, Mi, Ding, Jinhua, Pu, Yunqiao Joseph, Bryan, Anthony C., Rottmann, William, Winkeler, Kimberly A., Collins, Cassandra M., Singan, Vasanth, Lindquist, Erika A., Jawdy, Sara S., Gunter, Lee E., Engle, Nancy L., Yang, Xiaohan, Barry, Kerrie, Tschaplinski, Timothy J., Schmutz, Jeremy, Tuskan, Gerald A., Muchero, Wellington, & Chen, Jin-Gui. Overexpression of a Prefoldin β subunit gene reduces biomass recalcitrance in the bioenergy crop Populus. United States. doi:10.1111/pbi.13254.
Zhang, Jin, Xie, Meng, Li, Mi, Ding, Jinhua, Pu, Yunqiao Joseph, Bryan, Anthony C., Rottmann, William, Winkeler, Kimberly A., Collins, Cassandra M., Singan, Vasanth, Lindquist, Erika A., Jawdy, Sara S., Gunter, Lee E., Engle, Nancy L., Yang, Xiaohan, Barry, Kerrie, Tschaplinski, Timothy J., Schmutz, Jeremy, Tuskan, Gerald A., Muchero, Wellington, and Chen, Jin-Gui. Mon . "Overexpression of a Prefoldin β subunit gene reduces biomass recalcitrance in the bioenergy crop Populus". United States. doi:10.1111/pbi.13254.
@article{osti_1566924,
title = {Overexpression of a Prefoldin β subunit gene reduces biomass recalcitrance in the bioenergy crop Populus},
author = {Zhang, Jin and Xie, Meng and Li, Mi and Ding, Jinhua and Pu, Yunqiao Joseph and Bryan, Anthony C. and Rottmann, William and Winkeler, Kimberly A. and Collins, Cassandra M. and Singan, Vasanth and Lindquist, Erika A. and Jawdy, Sara S. and Gunter, Lee E. and Engle, Nancy L. and Yang, Xiaohan and Barry, Kerrie and Tschaplinski, Timothy J. and Schmutz, Jeremy and Tuskan, Gerald A. and Muchero, Wellington and Chen, Jin-Gui},
abstractNote = {Prefoldin (PFD) is a group II chaperonin that is ubiquitously present in the eukaryotic kingdom. Six subunits (PFD1-6) form a jellyfish-like heterohexameric PFD complex and function in protein folding and cytoskeleton organization. However, little is known about its function in plant cell wall-related processes. Here, we report the functional characterization of a PFD gene from em>Populus deltoides, designated as PdPFD2.2 There are two copies of PFD2 in em>Populus and PdPFD2.2 was ubiquitously expressed with high transcript abundance in the cambial region. PdPFD2.2 can physically interact with DELLA protein RGA1_8g, and its subcellular localization is affected by the interaction. In P. deltoides transgenic plants overexpressing PdPFD2.2, the lignin syringyl/guaiacyl ratio was increased, but cellulose content and crystallinity index were unchanged. In addition, the total released sugar (glucose and xylose) amounts were increased by 7.6% and 6.1%, respectively, in two transgenic lines. Transcriptomic and metabolomic analyses revealed that secondary metabolic pathways, including lignin and flavonoid biosynthesis, were affected by overexpressing PdPFD2.2. A total of eight hub transcription factors (TFs) were identified based on TF binding sites of differentially expressed genes in Populus transgenic plants overexpressing PdPFD2.2. In addition, several known cell wall-related TFs, such as MYB3, MYB4, MYB7, TT8, and XND1, were affected by overexpression of PdPFD2.2. These results suggest that overexpression of PdPFD2.2 can reduce biomass recalcitrance and PdPFD2.2 is a promising target for genetic engineering to improve feedstock characteristics to enhance biofuel conversion and reduce the cost of lignocellulosic biofuel production.},
doi = {10.1111/pbi.13254},
journal = {Plant Biotechnology Journal},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {9}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1111/pbi.13254

Save / Share:

Works referenced in this record:

Lignin modification improves fermentable sugar yields for biofuel production
journal, June 2007

  • Chen, Fang; Dixon, Richard A.
  • Nature Biotechnology, Vol. 25, Issue 7, p. 759-761
  • DOI: 10.1038/nbt1316

Biomass Recalcitrance: Engineering Plants and Enzymes for Biofuels Production
journal, February 2007

  • Himmel, M. E.; Ding, S.-Y.; Johnson, D. K.
  • Science, Vol. 315, Issue 5813, p. 804-807
  • DOI: 10.1126/science.1137016

Clustal W and Clustal X version 2.0
journal, September 2007


Lignin content in natural Populus variants affects sugar release
journal, March 2011

  • Studer, M. H.; DeMartini, J. D.; Davis, M. F.
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 15, p. 6300-6305
  • DOI: 10.1073/pnas.1009252108

mapman: a user-driven tool to display genomics data sets onto diagrams of metabolic pathways and other biological processes
journal, March 2004