Characterization of small microfluidic valves for studies of mechanical properties of bacteria
Abstract
Lab-on-a-chip platforms present many new opportunities to study bacterial cells and cellular assemblies. Here, the authors describe a new platform that allows us to apply uniaxial stress to individual bacterial cells while observing the cell and its subcellular assemblies using a high resolution optical microscope. The microfluidic chip consists of arrays of miniature pressure actuated valves. By placing a bacterium under one of such valves and partially closing the valve by externally applied pressure, the cell can be deformed. Although large pressure actuated valves used in integrated fluidic circuits have been extensively studied previously, here the authors downsize those microfluidic valves and use flow channels with rectangular cross-sections to maintain the bacteria in contact with cell culture medium during the experiments. The closure of these valves has not been characterized before. First, these valves are modeled using finite element analysis, and then compared the modeling results with the actual closing profiles of the valves, which is determined from absorption measurements. The measurements and modeling show with good agreement that the deflection of valves is a linear function of externally applied pressure and the deflection scales proportionally to the width of the flow channel. In addition to characterizing the valve, themore »
- Authors:
-
- Univ. of Tennessee, Knoxville, TN (United States). Dept. of Physics and Astronomy
- Univ. of Tennessee, Knoxville, TN (United States). Dept. of Mechanical, Aerospace and Biomedical Engineering
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Science (CNMS)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Sciences (CNMS)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); National Science Foundation (NSF)
- OSTI Identifier:
- 1265726
- Grant/Contract Number:
- AC05-00OR22725; MCB-1252890
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Journal of Vacuum Science and Technology B
- Additional Journal Information:
- Journal Volume: 33; Journal Issue: 6; Journal ID: ISSN 2166-2746
- Publisher:
- American Vacuum Society/AIP
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Channel flows; cell walls; polymers; bacteria; flow visualization
Citation Formats
Yang, Da, Greer, Clayton M., Jones, Branndon P., Jennings, Anna D., Retterer, Scott T., and Männik, Jaan. Characterization of small microfluidic valves for studies of mechanical properties of bacteria. United States: N. p., 2015.
Web. doi:10.1116/1.4929883.
Yang, Da, Greer, Clayton M., Jones, Branndon P., Jennings, Anna D., Retterer, Scott T., & Männik, Jaan. Characterization of small microfluidic valves for studies of mechanical properties of bacteria. United States. https://doi.org/10.1116/1.4929883
Yang, Da, Greer, Clayton M., Jones, Branndon P., Jennings, Anna D., Retterer, Scott T., and Männik, Jaan. Wed .
"Characterization of small microfluidic valves for studies of mechanical properties of bacteria". United States. https://doi.org/10.1116/1.4929883. https://www.osti.gov/servlets/purl/1265726.
@article{osti_1265726,
title = {Characterization of small microfluidic valves for studies of mechanical properties of bacteria},
author = {Yang, Da and Greer, Clayton M. and Jones, Branndon P. and Jennings, Anna D. and Retterer, Scott T. and Männik, Jaan},
abstractNote = {Lab-on-a-chip platforms present many new opportunities to study bacterial cells and cellular assemblies. Here, the authors describe a new platform that allows us to apply uniaxial stress to individual bacterial cells while observing the cell and its subcellular assemblies using a high resolution optical microscope. The microfluidic chip consists of arrays of miniature pressure actuated valves. By placing a bacterium under one of such valves and partially closing the valve by externally applied pressure, the cell can be deformed. Although large pressure actuated valves used in integrated fluidic circuits have been extensively studied previously, here the authors downsize those microfluidic valves and use flow channels with rectangular cross-sections to maintain the bacteria in contact with cell culture medium during the experiments. The closure of these valves has not been characterized before. First, these valves are modeled using finite element analysis, and then compared the modeling results with the actual closing profiles of the valves, which is determined from absorption measurements. The measurements and modeling show with good agreement that the deflection of valves is a linear function of externally applied pressure and the deflection scales proportionally to the width of the flow channel. In addition to characterizing the valve, the authors show at a proof-of-principle level that it can be used to deform a bacterial cell at considerable magnitude. They found the largest deformations in 5 μm wide channels where the bacterial width and length increase by 1.6 and 1.25 times, respectively. Narrower and broader channels are less optimal for these studies. Finally, the platform presents a promising approach to probe, in a quantitative and systematic way, the mechanical properties of not only bacterial cells but possibly also yeast and other single-celled organisms.},
doi = {10.1116/1.4929883},
journal = {Journal of Vacuum Science and Technology B},
number = 6,
volume = 33,
place = {United States},
year = {Wed Sep 02 00:00:00 EDT 2015},
month = {Wed Sep 02 00:00:00 EDT 2015}
}
Web of Science
Works referenced in this record:
Zooming in to see the bigger picture: Microfluidic and nanofabrication tools to study bacteria
journal, October 2014
- Hol, Felix J. H.; Dekker, Cees
- Science, Vol. 346, Issue 6208
Cells on chips
journal, July 2006
- El-Ali, Jamil; Sorger, Peter K.; Jensen, Klavs F.
- Nature, Vol. 442, Issue 7101
Dissecting biological "dark matter" with single-cell genetic analysis of rare and uncultivated TM7 microbes from the human mouth
journal, July 2007
- Marcy, Y.; Ouverney, C.; Bik, E. M.
- Proceedings of the National Academy of Sciences, Vol. 104, Issue 29, p. 11889-11894
A programmable droplet-based microfluidic device applied to multiparameter analysis of single microbes and microbial communities
journal, April 2012
- Leung, K.; Zahn, H.; Leaver, T.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 20
Probing electron transfer mechanisms in Shewanella oneidensis MR-1 using a nanoelectrode platform and single-cell imaging
journal, September 2010
- Jiang, X.; Hu, J.; Fitzgerald, L. A.
- Proceedings of the National Academy of Sciences, Vol. 107, Issue 39
Probing single- to multi-cell level charge transport in Geobacter sulfurreducens DL-1
journal, November 2013
- Jiang, Xiaocheng; Hu, Jinsong; Petersen, Emily R.
- Nature Communications, Vol. 4, Issue 1
Why and How Bacteria Localize Proteins
journal, November 2009
- Shapiro, L.; McAdams, H. H.; Losick, R.
- Science, Vol. 326, Issue 5957
Cell Division Intersects with Cell Geometry
journal, July 2010
- Moseley, James B.; Nurse, Paul
- Cell, Vol. 142, Issue 2
Spatial coordination between chromosomes and cell division proteins in Escherichia coli
journal, April 2015
- Männik, Jaan; Bailey, Matthew W.
- Frontiers in Microbiology, Vol. 6
Robust Growth of Escherichia coli
journal, June 2010
- Wang, Ping; Robert, Lydia; Pelletier, James
- Current Biology, Vol. 20, Issue 12
Electron beam fabrication of a microfluidic device for studying submicron-scale bacteria
journal, January 2013
- Moolman, M.; Huang, Zhuangxiong; Krishnan, Sriram
- Journal of Nanobiotechnology, Vol. 11, Issue 1
Microfluidic chemostat for measuring single cell dynamics in bacteria
journal, January 2013
- Long, Zhicheng; Nugent, Eileen; Javer, Avelino
- Lab on a Chip, Vol. 13, Issue 5
High-throughput gene expression analysis at the level of single proteins using a microfluidic turbidostat and automated cell tracking
journal, February 2013
- Ullman, G.; Wallden, M.; Marklund, E. G.
- Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 368, Issue 1611
Microfluidic devices for measuring gene network dynamics in single cells
journal, August 2009
- Bennett, Matthew R.; Hasty, Jeff
- Nature Reviews Genetics, Vol. 10, Issue 9
Controlling the Shape of Filamentous Cells of Escherichia coli
journal, August 2005
- Takeuchi, Shoji; DiLuzio, Willow R.; Weibel, Douglas B.
- Nano Letters, Vol. 5, Issue 9
Bending forces plastically deform growing bacterial cell walls
journal, April 2014
- Amir, A.; Babaeipour, F.; McIntosh, D. B.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 16
Deformation of Filamentous Escherichia coli Cells in a Microfluidic Device: A New Technique to Study Cell Mechanics
journal, January 2014
- Caspi, Yaron
- PLoS ONE, Vol. 9, Issue 1
Bacterial growth and motility in sub-micron constrictions
journal, August 2009
- Mannik, J.; Driessen, R.; Galajda, P.
- Proceedings of the National Academy of Sciences, Vol. 106, Issue 35
Robustness and accuracy of cell division in Escherichia coli in diverse cell shapes
journal, April 2012
- Mannik, J.; Wu, F.; Hol, F. J. H.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 18
Monolithic Microfabricated Valves and Pumps by Multilayer Soft Lithography
journal, April 2000
- Unger, M. A.
- Science, Vol. 288, Issue 5463
Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants the Keio collection
journal, February 2006
- Baba, Tomoya; Ara, Takeshi; Hasegawa, Miki
- Molecular Systems Biology, Vol. 2, Article No. 2006.0008
Evidence for Divisome Localization Mechanisms Independent of the Min System and SlmA in Escherichia coli
journal, August 2014
- Bailey, Matthew W.; Bisicchia, Paola; Warren, Boyd T.
- PLoS Genetics, Vol. 10, Issue 8
Scaling properties of a low-actuation pressure microfluidic valve
journal, January 2004
- Studer, Vincent; Hang, Giao; Pandolfi, Anna
- Journal of Applied Physics, Vol. 95, Issue 1
Experimentally validated quantitative linear model for the device physics of elastomeric microfluidic valves
journal, March 2007
- Kartalov, Emil P.; Scherer, Axel; Quake, Stephen R.
- Journal of Applied Physics, Vol. 101, Issue 6
Mechanical characterization of bulk Sylgard 184 for microfluidics and microengineering
journal, February 2014
- Johnston, I. D.; McCluskey, D. K.; Tan, C. K. L.
- Journal of Micromechanics and Microengineering, Vol. 24, Issue 3
Effects of strain rate, mixing ratio, and stress–strain definition on the mechanical behavior of the polydimethylsiloxane (PDMS) material as related to its biological applications
journal, December 2008
- Khanafer, Khalil; Duprey, Ambroise; Schlicht, Marty
- Biomedical Microdevices, Vol. 11, Issue 2
Deformation of PDMS membrane and microcantilever by a water droplet: Comparison between Mooney–Rivlin and linear elastic constitutive models
journal, April 2009
- Yu, Ying-Song; Zhao, Ya-Pu
- Journal of Colloid and Interface Science, Vol. 332, Issue 2, p. 467-476
Quantitative 3-dimensional profiling of channel networks within transparent ‘lab-on-a-chip’ microreactors using a digital imaging method
journal, January 2001
- Broadwell, Ian; Fletcher, Paul D. I.; Haswell, Stephen J.
- Lab Chip, Vol. 1, Issue 1
Determination of Brilliant Blue FCF in food and cosmetic samples by ionic liquid independent disperse liquid–liquid micro-extraction
journal, January 2013
- Guo, Jingbo; Wu, Hao; Du, Liming
- Analytical Methods, Vol. 5, Issue 16
Quantitative full-colour transmitted light microscopy and dyes for concentration mapping and measurement of diffusion coefficients in microfluidic architectures
journal, January 2012
- Werts, Martinus H. V.; Raimbault, Vincent; Texier-Picard, Rozenn
- Lab on a Chip, Vol. 12, Issue 4
Works referencing / citing this record:
Analysis of Factors Limiting Bacterial Growth in PDMS Mother Machine Devices
journal, May 2018
- Yang, Da; Jennings, Anna D.; Borrego, Evalynn
- Frontiers in Microbiology, Vol. 9
Analysis of Factors Limiting Bacterial Growth in PDMS Mother Machine Devices
journal, May 2018
- Yang, Da; Jennings, Anna D.; Borrego, Evalynn
- Frontiers in Microbiology, Vol. 9