Improving Sugar Yields and Reducing Enzyme Loadings in the Deacetylation and Mechanical Refining (DMR) Process through Multistage Disk and Szego Refining and Corresponding Techno-Economic Analysis
Abstract
Deacetylation and mechanical refining (DMR) has the potential to be a highly efficient biochemical conversion process for converting biomass to low toxicity, high concentration sugar streams. To increase the cost-effectiveness of the DMR process, improvements in enzymatic sugar yields are needed, in addition to reducing the refining energy consumed, and decreasing the enzyme usage. In this study, a second refining step utilizing a Szego mill was introduced, resulting in significant improvements in sugar yields in enzymatic hydrolysis at equivalent or lower refining energy inputs. The multistage DMR process increased the monomeric glucose and xylose yields to approximately 90% and 84%, respectively, with an energy consumption of 200 kWh/ODMT. SEM imaging revealed that Szego milling caused significant surface disruption and severe maceration and delamination of the biomass structure. Our results show that the DMR process is a very promising process for the biorefinery industry in terms of economic feasibility.
- Authors:
-
- National Bioenergy Center, National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, Colorado 80401, United States
- Bioscience Center, National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, Colorado 80401, United States
- Department of Chemical Engineering and Applied Chemistry, University of Toronto, 200 College Street, Toronto, Ontario M5S 3E5, Canada
- Department of Forest Biomaterials, North Carolina State University, 2820 Faucette Drive, Campus box 8005, Raleigh, North Carolina 27695, United States
- Publication Date:
- Research Org.:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE); NREL (National Renewable Energy Laboratory (NREL), Golden, CO (United States))
- OSTI Identifier:
- 1227578
- Alternate Identifier(s):
- OSTI ID: 1235670
- Report Number(s):
- NREL/JA-5100-64773
Journal ID: ISSN 2168-0485
- Grant/Contract Number:
- AC36-08GO28308
- Resource Type:
- Published Article
- Journal Name:
- ACS Sustainable Chemistry & Engineering
- Additional Journal Information:
- Journal Name: ACS Sustainable Chemistry & Engineering Journal Volume: 4 Journal Issue: 1; Journal ID: ISSN 2168-0485
- Publisher:
- American Chemical Society (ACS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; biorefinery; pretreatment; biomass saccharification; mechanical refining; Szego milling; PFI milling; disk refining; plant derived food; peptides and proteins; carbohydrates; biomass; energy; deacetylation; alkaline pretreatment; ethanol; sugar; techno-economic analysis
Citation Formats
Chen, Xiaowen, Wang, Wei, Ciesielski, Peter, Trass, Olev, Park, Sunkyu, Tao, Ling, and Tucker, Melvin P. Improving Sugar Yields and Reducing Enzyme Loadings in the Deacetylation and Mechanical Refining (DMR) Process through Multistage Disk and Szego Refining and Corresponding Techno-Economic Analysis. United States: N. p., 2015.
Web. doi:10.1021/acssuschemeng.5b01242.
Chen, Xiaowen, Wang, Wei, Ciesielski, Peter, Trass, Olev, Park, Sunkyu, Tao, Ling, & Tucker, Melvin P. Improving Sugar Yields and Reducing Enzyme Loadings in the Deacetylation and Mechanical Refining (DMR) Process through Multistage Disk and Szego Refining and Corresponding Techno-Economic Analysis. United States. https://doi.org/10.1021/acssuschemeng.5b01242
Chen, Xiaowen, Wang, Wei, Ciesielski, Peter, Trass, Olev, Park, Sunkyu, Tao, Ling, and Tucker, Melvin P. Wed .
"Improving Sugar Yields and Reducing Enzyme Loadings in the Deacetylation and Mechanical Refining (DMR) Process through Multistage Disk and Szego Refining and Corresponding Techno-Economic Analysis". United States. https://doi.org/10.1021/acssuschemeng.5b01242.
@article{osti_1227578,
title = {Improving Sugar Yields and Reducing Enzyme Loadings in the Deacetylation and Mechanical Refining (DMR) Process through Multistage Disk and Szego Refining and Corresponding Techno-Economic Analysis},
author = {Chen, Xiaowen and Wang, Wei and Ciesielski, Peter and Trass, Olev and Park, Sunkyu and Tao, Ling and Tucker, Melvin P.},
abstractNote = {Deacetylation and mechanical refining (DMR) has the potential to be a highly efficient biochemical conversion process for converting biomass to low toxicity, high concentration sugar streams. To increase the cost-effectiveness of the DMR process, improvements in enzymatic sugar yields are needed, in addition to reducing the refining energy consumed, and decreasing the enzyme usage. In this study, a second refining step utilizing a Szego mill was introduced, resulting in significant improvements in sugar yields in enzymatic hydrolysis at equivalent or lower refining energy inputs. The multistage DMR process increased the monomeric glucose and xylose yields to approximately 90% and 84%, respectively, with an energy consumption of 200 kWh/ODMT. SEM imaging revealed that Szego milling caused significant surface disruption and severe maceration and delamination of the biomass structure. Our results show that the DMR process is a very promising process for the biorefinery industry in terms of economic feasibility.},
doi = {10.1021/acssuschemeng.5b01242},
journal = {ACS Sustainable Chemistry & Engineering},
number = 1,
volume = 4,
place = {United States},
year = {Wed Dec 02 00:00:00 EST 2015},
month = {Wed Dec 02 00:00:00 EST 2015}
}
https://doi.org/10.1021/acssuschemeng.5b01242
Web of Science
Works referenced in this record:
Yield-determining factors in high-solids enzymatic hydrolysis of lignocellulose
journal, January 2009
- Kristensen, Jan B.; Felby, Claus; Jørgensen, Henning
- Biotechnology for Biofuels, Vol. 2, Issue 1
Bioethanol production: Status and prospects
journal, February 1997
- McMillan, James D.
- Renewable Energy, Vol. 10, Issue 2-3
Modeling energy consumption for the generation of microfibres from bleached kraft pulp fibres in a PFI mill
journal, April 2007
- Chakraborty, Ayan; Sain, Mohini M.; Kortschot, Mark T.
- BioResources, Vol. 2, Issue 2
The effect of delignification of forest biomass on enzymatic hydrolysis
journal, October 2011
- Yu, Zhiying; Jameel, Hasan; Chang, Hou-min
- Bioresource Technology, Vol. 102, Issue 19
Dilute-Sulfuric Acid Pretreatment of Corn Stover in Pilot-Scale Reactor: Investigation of Yields, Kinetics, and Enzymatic Digestibilities of Solids
journal, January 2003
- Schell, Daniel J.; Farmer, Jody; Newman, Millie
- Applied Biochemistry and Biotechnology, Vol. 105, Issue 1-3, p. 69-86
Development of a high-throughput method to evaluate the impact of inhibitory compounds from lignocellulosic hydrolysates on the growth of Zymomonas mobilis
journal, December 2009
- Franden, Mary Ann; Pienkos, Philip T.; Zhang, Min
- Journal of Biotechnology, Vol. 144, Issue 4
Inhibition of ethanol-producing yeast and bacteria by degradation products produced during pre-treatment of biomass
journal, August 2004
- Klinke, H. B.; Thomsen, A. B.; Ahring, B. K.
- Applied Microbiology and Biotechnology, Vol. 66, Issue 1, p. 10-26
Techno-economic analysis of the deacetylation and disk refining process: characterizing the effect of refining energy and enzyme usage on minimum sugar selling price and minimum ethanol selling price
journal, October 2015
- Chen, Xiaowen; Shekiro, Joseph; Pschorn, Thomas
- Biotechnology for Biofuels, Vol. 8, Issue 1
Pretreatment of woody biomass for biofuel production: energy efficiency, technologies, and recalcitrance
journal, May 2010
- Zhu, J. Y.; Pan, Xuejun; Zalesny, Ronald S.
- Applied Microbiology and Biotechnology, Vol. 87, Issue 3
Green liquor extraction of hemicelluloses from southern pine in an Integrated Forest Biorefinery
journal, January 2010
- Yoon, Sung-Hoon; van Heiningen, Adriaan
- Journal of Industrial and Engineering Chemistry, Vol. 16, Issue 1
Process Design and Economics for Biochemical Conversion of Lignocellulosic Biomass to Ethanol: Dilute-Acid Pretreatment and Enzymatic Hydrolysis of Corn Stover
report, March 2011
- Humbird, D.; Davis, R.; Tao, L.
Lignocellulosic Biomass to Ethanol Process Design and Economics Utilizing Co-Current Dilute Acid Prehydrolysis and Enzymatic Hydrolysis for Corn Stover
report, June 2002
- Aden, A.; Ruth, M.; Ibsen, K.
- NREL/TP-510-32438
Alkaline Pretreatment of Corn Stover: Bench-Scale Fractionation and Stream Characterization
journal, May 2014
- Karp, Eric M.; Donohoe, Bryon S.; O’Brien, Marykate H.
- ACS Sustainable Chemistry & Engineering, Vol. 2, Issue 6, p. 1481-1491
Effects of Temperature and Moisture on Dilute-Acid Steam Explosion Pretreatment of Corn Stover and Cellulase Enzyme Digestibility
journal, January 2003
- Tucker, Melvin P.; Kim, Kyoung H.; Newman, Mildred M.
- Applied Biochemistry and Biotechnology, Vol. 105, Issue 1-3
Engineering plant cell walls: tuning lignin monomer composition for deconstructable biofuel feedstocks or resilient biomaterials
journal, January 2014
- Ciesielski, Peter N.; Resch, Michael G.; Hewetson, Barron
- Green Chemistry, Vol. 16, Issue 5, p. 2627-2635
A highly efficient dilute alkali deacetylation and mechanical (disc) refining process for the conversion of renewable biomass to lower cost sugars
journal, January 2014
- Chen, Xiaowen; Shekiro, Joseph; Pschorn, Thomas
- Biotechnology for Biofuels, Vol. 7, Issue 1
Comparison of different mechanical refining technologies on the enzymatic digestibility of low severity acid pretreated corn stover
journal, November 2013
- Chen, Xiaowen; Kuhn, Erik; Wang, Wei
- Bioresource Technology, Vol. 147
Effect of mechanical disruption on the effectiveness of three reactors used for dilute acid pretreatment of corn stover Part 1: chemical and physical substrate analysis
journal, January 2014
- Wang, Wei; Chen, Xiaowen; Donohoe, Bryon S.
- Biotechnology for Biofuels, Vol. 7, Issue 1
Novel Green Liquor Pretreatment of Loblolly Pine Chips to Facilitate Enzymatic Hydrolysis into Fermentable Sugars for Ethanol Production
journal, August 2010
- Wu, Shu-fang; Chang, Hou-min; Jameel, Hasan
- Journal of Wood Chemistry and Technology, Vol. 30, Issue 3
Evaluation of Clean Fractionation Pretreatment for the Production of Renewable Fuels and Chemicals from Corn Stover
journal, May 2014
- Katahira, Rui; Mittal, Ashutosh; McKinney, Kellene
- ACS Sustainable Chemistry & Engineering, Vol. 2, Issue 6
Clean Fractionation Pretreatment Reduces Enzyme Loadings for Biomass Saccharification and Reveals the Mechanism of Free and Cellulosomal Enzyme Synergy
journal, May 2014
- Resch, Michael G.; Donohoe, Bryon S.; Ciesielski, Peter N.
- ACS Sustainable Chemistry & Engineering, Vol. 2, Issue 6
Sulfite pretreatment (SPORL) for robust enzymatic saccharification of spruce and red pine
journal, April 2009
- Zhu, J. Y.; Pan, X. J.; Wang, G. S.
- Bioresource Technology, Vol. 100, Issue 8