Kinetics and Rheological Behavior of Higher Solid (Solids >20%) Enzymatic Hydrolysis Reactions Using Dilute Acid Pretreated, Deacetylation and Disk Refined, and Deacetylation and Mechanical Refined (DMR) Corn Stover Slurries

Chen, Xiaowen; Crawford, Nathan; Wang, Wei; Kuhn, Erik; Sievers, David; Tao, Ling; Tucker, Melvin

doi:10.1021/acssuschemeng.8b05391

Title: Kinetics and Rheological Behavior of Higher Solid (Solids >20%) Enzymatic Hydrolysis Reactions Using Dilute Acid Pretreated, Deacetylation and Disk Refined, and Deacetylation and Mechanical Refined (DMR) Corn Stover Slurries

Full Record
Other Related Research

Abstract

Many potential biochemical pathways producing advanced hydrocarbon fuels from renewable lignocellulosic biomass require hydrolysates with high sugar concentrations and low toxicity, enabling flexible fermentation strategies, such as fed-batch fermentations capable of producing high product titers and production rates during fermentation. High sugar concentrations also increase the osmotic pressure in the hydrolysates, thus helping to decrease contamination issues. We have shown the production of high sugar concentrations directly from biomass without the need for energy/capital intensive concentration, conditioning, and/or purification steps. In our previous work, we successfully demonstrated high biomass-derived sugar concentrations (over 230 g/L fermentable sugars) in enzymatic hydrolysis from high solid (>20 wt % insoluble solids) digestions of dilute alkali deacetylated and mechanically refined (DMR) corn stover slurries. The goal of this work was to understand the effects of initial solid loadings on differently pretreated corn stover substrates on the rheological property changes of enzymatic-hydrolyzed slurries as well as the rates and yields of the enzymatic hydrolysis reactions. We performed high-solid enzymatic hydrolysis using deacetylated and disk refined (DDR), DMR, and dilute acid pretreated (DAP) corn stover substrates at four different initial total solid loadings, 17, 22, 27, and 32%. Slurry samples were collected at regular intervals for measurementsmore »« less

Authors:

Chen, Xiaowen ^[1]; Crawford, Nathan ^[2]; Wang, Wei ^[1]; Kuhn, Erik ^[1]; Sievers, David ^[1]; Tao, Ling ^[1]; Tucker, Melvin ^[1]

National Renewable Energy Lab. (NREL), Golden, CO (United States)
Thermo Fisher Scientific, Lafayette, CO (United States)

Publication Date:: Wed Dec 12 00:00:00 EST 2018

Research Org.:: National Renewable Energy Lab. (NREL), Golden, CO (United States)

Sponsoring Org.:: USDOE Office of Energy Efficiency and Renewable Energy (EERE)

OSTI Identifier:: 1491371

Report Number(s):: NREL/JA-5100-70046
Journal ID: ISSN 2168-0485

Grant/Contract Number:: AC36-08GO28308

Resource Type:: Accepted Manuscript

Journal Name:: ACS Sustainable Chemistry & Engineering

Additional Journal Information:: Journal Volume: 7; Journal Issue: 1; Journal ID: ISSN 2168-0485

Publisher:: American Chemical Society (ACS)

Country of Publication:: United States

Language:: English

Subject:: 09 BIOMASS FUELS; biorefinery; kinetics; mechanical refining; pretreatment; rheology

Citation Formats


                    Chen, Xiaowen, Crawford, Nathan, Wang, Wei, Kuhn, Erik, Sievers, David, Tao, Ling, and Tucker, Melvin. Kinetics and Rheological Behavior of Higher Solid (Solids >20%) Enzymatic Hydrolysis Reactions Using Dilute Acid Pretreated, Deacetylation and Disk Refined, and Deacetylation and Mechanical Refined (DMR) Corn Stover Slurries.  United States: N. p., 2018. 
Web.  doi:10.1021/acssuschemeng.8b05391.

Copy to clipboard


                    Chen, Xiaowen, Crawford, Nathan, Wang, Wei, Kuhn, Erik, Sievers, David, Tao, Ling, & Tucker, Melvin. Kinetics and Rheological Behavior of Higher Solid (Solids >20%) Enzymatic Hydrolysis Reactions Using Dilute Acid Pretreated, Deacetylation and Disk Refined, and Deacetylation and Mechanical Refined (DMR) Corn Stover Slurries.  United States.  https://doi.org/10.1021/acssuschemeng.8b05391

Copy to clipboard


                    Chen, Xiaowen, Crawford, Nathan, Wang, Wei, Kuhn, Erik, Sievers, David, Tao, Ling, and Tucker, Melvin. Wed .  
"Kinetics and Rheological Behavior of Higher Solid (Solids >20%) Enzymatic Hydrolysis Reactions Using Dilute Acid Pretreated, Deacetylation and Disk Refined, and Deacetylation and Mechanical Refined (DMR) Corn Stover Slurries".  United States.  https://doi.org/10.1021/acssuschemeng.8b05391.  https://www.osti.gov/servlets/purl/1491371.

Copy to clipboard


                    
@article{osti_1491371,

  title        = {Kinetics and Rheological Behavior of Higher Solid (Solids >20%) Enzymatic Hydrolysis Reactions Using Dilute Acid Pretreated, Deacetylation and Disk Refined, and Deacetylation and Mechanical Refined (DMR) Corn Stover Slurries},

  author       = {Chen, Xiaowen and Crawford, Nathan and Wang, Wei and Kuhn, Erik and Sievers, David and Tao, Ling and Tucker, Melvin},

  abstractNote = {Many potential biochemical pathways producing advanced hydrocarbon fuels from renewable lignocellulosic biomass require hydrolysates with high sugar concentrations and low toxicity, enabling flexible fermentation strategies, such as fed-batch fermentations capable of producing high product titers and production rates during fermentation. High sugar concentrations also increase the osmotic pressure in the hydrolysates, thus helping to decrease contamination issues. We have shown the production of high sugar concentrations directly from biomass without the need for energy/capital intensive concentration, conditioning, and/or purification steps. In our previous work, we successfully demonstrated high biomass-derived sugar concentrations (over 230 g/L fermentable sugars) in enzymatic hydrolysis from high solid (>20 wt % insoluble solids) digestions of dilute alkali deacetylated and mechanically refined (DMR) corn stover slurries. The goal of this work was to understand the effects of initial solid loadings on differently pretreated corn stover substrates on the rheological property changes of enzymatic-hydrolyzed slurries as well as the rates and yields of the enzymatic hydrolysis reactions. We performed high-solid enzymatic hydrolysis using deacetylated and disk refined (DDR), DMR, and dilute acid pretreated (DAP) corn stover substrates at four different initial total solid loadings, 17, 22, 27, and 32%. Slurry samples were collected at regular intervals for measurements of monomeric and oligomeric sugar concentrations (glucose and xylose), particle size distributions, viscosities, and yield stresses. We produced over 270 g/L of fermentable, monomeric sugars (157 g/L of glucose and 114 g/L of xylose) at 32 wt % total insoluble solid during enzymatic hydrolysis of the DMR substrates. The pumpabilities of the digested, high-solid enzymatic hydrolysates were shown to be comparable to other commercially relevant slurry streams, such as honey, peanut butter, and ketchup from the food industry. The conversion and rheological results indicate that high-solid enzymatic hydrolysis (~33 wt %) of DMR substrates is a promising and scalable technology for producing high sugar concentration slurries from lignocellulosic biomass.},

  doi          = {10.1021/acssuschemeng.8b05391},

  journal      = {ACS Sustainable Chemistry & Engineering},

  number       = 1,

  volume       = 7,

  place        = {United States},

  year         = {Wed Dec 12 00:00:00 EST 2018},

  month        = {Wed Dec 12 00:00:00 EST 2018}

}

Copy to clipboard

Journal Article:

Free Publicly Available Full Text

Accepted Manuscript (DOE)

Publisher's Version of Record

https://doi.org/10.1021/acssuschemeng.8b05391

Other availability

Search WorldCat to find libraries that may hold this journal

Citation Metrics:

Cited by: 9 works

Citation information provided by
Web of Science

Save / Share:

Export Metadata

Save to My Library

Similar Records in DOE PAGES and OSTI.GOV collections:

DMR (deacetylation and mechanical refining) processing of corn stover achieves high monomeric sugar concentrations (230 g L^-1) during enzymatic hydrolysis and high ethanol concentrations (>10% v/v) during fermentation without hydrolysate purification or concentration

Journal Article Chen, Xiaowen ; Kuhn, Erik ; Jennings, Edward W. ; ... - Energy & Environmental Science

Distilling and purifying ethanol and other products from second generation lignocellulosic biorefineries adds significant capital and operating costs to biofuel production. The energy usage associated with distillation negatively affects plant gate costs and causes environmental and life-cycle impacts, and the lower titers in fermentation caused by lower sugar concentrations from pretreatment and enzymatic hydrolysis increase energy and water usage and ethanol production costs. In addition, lower ethanol titers increase the volumes required for enzymatic hydrolysis and fermentation vessels increase capital expenditure (CAPEX). Therefore, increasing biofuel titers has been a research focus in renewable biofuel production for several decades. In thismore »« less
Cited by 119
https://doi.org/10.1039/C5EE03718B

Full Text Available
Enhanced Bioconversion in Herbaceous Feedstocks from Impregnation of Co-Catalytic Lewis Acid and Deacetylation and Mechanical Refining

Conference Nagle, Nicholas J ; Chen, Xiaowen ; Tucker, Melvin P ; ...

Improvements in conversion processes are needed to further reduce process complexity, biorefinery capital and operating (CAPEX/OPEX) costs, lower enzyme usage. Previous work has demonstrated that deacetylation and mechanical refining (DMR) process effectively deconstructs herbaceous biomass while producing highly digestible lignocellulosic slurries, with high titers of monomeric sugars, at reduced enzyme loadings (Poster 553 Chen at al,). We evaluated a co-catalytic approach using three Lewis acids: Fe+2, Fe+3, and Al+3, in sulfate form, at 1 mM, 5 mM and 10 mM concentrations. Lewis acids were impregnated into corn stover, followed by deacetylation with NaOH, and mechanically refined, prior to enzymatic hydrolysismore »« less
Full Text Available
Deacetylation and Mechanical Refining (DMR) and Deacetylation and Dilute Acid (DDA) Pretreatment of Corn Stover, Switchgrass, and a 50/50 Corn Stover/Switchgrass Blend

Journal Article Kuhn, Erik M. ; Chen, Xiaowen ; Tucker, Melvin P. - ACS Sustainable Chemistry & Engineering

The most economical feedstock available to future lignocellulosic biorefineries may be a feedstock blend. Corn stover, switchgrass, and a 50:50 blend of corn stover and switchgrass were subjected to three different dilute alkali deacetylation conditions in a 90 L reactor prior to either mechanical refining (DMR) in a disc refiner followed by a Szego mill or dilute acid pretreatment (DDA) at five different conditions in a 4 L batch steam explosion reactor. The forty-five DDA and nine DMR slurries were subjected to high solids enzymatic hydrolysis at an enzyme loading of 15 mg protein per gram cellulose using Novozymes Cellicmore »« less
https://doi.org/10.1021/acssuschemeng.0c00894
Direct Conversion of Delignified Biomass into Non-Volatile Products Using Thermophilic Bacteria

Conference Hengge, Neal N. ; Moreno Garcia, Lizzette ; Arnolds, Katie ; ...

Butyric acid (BA) can be used as a platform intermediate for diesel and sustainable aviation fuels (SAFs) as well as a precursor for diverse commodity chemicals for the production of polymers, fibers, solvents, and preservatives. This work presents a co-culture-based bioprocess for the production of BA from corn stover by two thermophilic bacteria, Clostridium thermocellum, a well-known efficient degrader of insoluble and oligomeric cellulosic substrates, and Clostridium thermobutyricum, a highly efficient BA producer from monomeric sugars. First, we tested the capability of C. thermocellum to deconstruct three different biomass streams from the NREL pilot plant pretreatment process -raw, deacetylated, andmore »« less
Full Text Available
Development and Characterization of a High-Solids Deacetylation Process

Journal Article Shekiro, III, Joseph ; Chen, Xiaowen ; Smith, Holly ; ... - Sustainable Chemical Processes

Dilute-acid pretreatment has proven to be a robust means of converting herbaceous feedstock to fermentable sugars. However, it also releases acetic acid, a known fermentation inhibitor, from acetyl groups present in the biomass. A mild, dilute alkaline extraction stage was implemented prior to acid pretreatment to separate acetic acid from the hydrolysate sugar stream. This step, termed deacetylation, improved the glucose and xylose yields from enzymatic hydrolysis and ethanol yields from fermentation of the sugars relative to the control experiments using dilute-acid pretreatment of native corn stover without deacetylation. While promising, deacetylation as it was historically practiced is conducted atmore »« less
https://doi.org/10.1186/s40508-016-0049-6

Full Text Available

Similar Records