Modified cellulose synthase gene from Arabidopsis thaliana confers herbicide resistance to plants
Abstract
Cellulose synthase ("CS"), a key enzyme in the biosynthesis of cellulose in plants is inhibited by herbicides comprising thiazolidinones such as 5-tert-butyl-carbamoyloxy-3-(3-trifluromethyl)phenyl-4-thiazolidinone (TZ), isoxaben and 2,6-dichlorobenzonitrile (DCB). Two mutant genes encoding isoxaben and TZ-resistant cellulose synthase have been isolated from isoxaben and TZ-resistant Arabidopsis thaliana mutants. When compared with the gene coding for isoxaben or TZ-sensitive cellulose synthase, one of the resistant CS genes contains a point mutation, wherein glycine residue 998 is replaced by an aspartic acid. The other resistant mutation is due to a threonine to isoleucine change at amino acid residue 942. The mutant CS gene can be used to impart herbicide resistance to a plant; thereby permitting the utilization of the herbicide as a single application at a concentration which ensures the complete or substantially complete killing of weeds, while leaving the transgenic crop plant essentially undamaged.
- Inventors:
-
- Portola Valley, CA
- Golm, DE
- Issue Date:
- Research Org.:
- Carnegie Institute of Washington
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 912868
- Patent Number(s):
- 7241878
- Application Number:
- 10/721,996
- Assignee:
- U.S. Department of Energy (Washington, DC)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- FG02-94ER20133
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Somerville, Chris R, and Scheible, Wolf. Modified cellulose synthase gene from Arabidopsis thaliana confers herbicide resistance to plants. United States: N. p., 2007.
Web.
Somerville, Chris R, & Scheible, Wolf. Modified cellulose synthase gene from Arabidopsis thaliana confers herbicide resistance to plants. United States.
Somerville, Chris R, and Scheible, Wolf. Tue .
"Modified cellulose synthase gene from Arabidopsis thaliana confers herbicide resistance to plants". United States. https://www.osti.gov/servlets/purl/912868.
@article{osti_912868,
title = {Modified cellulose synthase gene from Arabidopsis thaliana confers herbicide resistance to plants},
author = {Somerville, Chris R and Scheible, Wolf},
abstractNote = {Cellulose synthase ("CS"), a key enzyme in the biosynthesis of cellulose in plants is inhibited by herbicides comprising thiazolidinones such as 5-tert-butyl-carbamoyloxy-3-(3-trifluromethyl)phenyl-4-thiazolidinone (TZ), isoxaben and 2,6-dichlorobenzonitrile (DCB). Two mutant genes encoding isoxaben and TZ-resistant cellulose synthase have been isolated from isoxaben and TZ-resistant Arabidopsis thaliana mutants. When compared with the gene coding for isoxaben or TZ-sensitive cellulose synthase, one of the resistant CS genes contains a point mutation, wherein glycine residue 998 is replaced by an aspartic acid. The other resistant mutation is due to a threonine to isoleucine change at amino acid residue 942. The mutant CS gene can be used to impart herbicide resistance to a plant; thereby permitting the utilization of the herbicide as a single application at a concentration which ensures the complete or substantially complete killing of weeds, while leaving the transgenic crop plant essentially undamaged.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jul 10 00:00:00 EDT 2007},
month = {Tue Jul 10 00:00:00 EDT 2007}
}
Works referenced in this record:
A Second Locus, Ixr B1 in Arabidopsis thaliana, that Confers Resistance to the Herbicide Isoxaben
journal, March 1990
- Heim, Dale R.; Roberts, Jean L.; Pike, Philip D.
- Plant Physiology, Vol. 92, Issue 3
Modifications of cellulose synthase confer resistance to isoxaben and thiazolidinone herbicides in Arabidopsis Ixr1 mutants
journal, August 2001
- Scheible, W. -R.; Eshed, R.; Richmond, T.
- Proceedings of the National Academy of Sciences, Vol. 98, Issue 18