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Title: Regulation of galactan synthase expression to modify galactan content in plants

Patent ·
OSTI ID:1376066

The disclosure provides methods of engineering plants to modulate galactan content. Specifically, the disclosure provides methods for engineering a plant to increase the galactan content in a plant tissue by inducing expression of beta-1,4-galactan synthase (GALS), modulated by a heterologous promoter. Further disclosed are the methods of modulating expression level of GALS under the regulation of a transcription factor, as well as overexpression of UDP-galactose epimerse in the same plant tissue. Tissue specific promoters and transcription factors can be used in the methods are also provided.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC02-05CH11231
Assignee:
The Regents of the University of California
Patent Number(s):
9,738,901
Application Number:
14/399,880
OSTI ID:
1376066
Resource Relation:
Patent File Date: 2013 May 10
Country of Publication:
United States
Language:
English

References (15)

Plant UDP-glucose epimerases patent January 2006
Nonnatural galactomannans and methods of use patent September 2009
Plant having reduced lignin and cellulose contents without reducing glucan content, method of producing the same and utilization thereof patent May 2012
Method to produce para-hydroxybenzoic acid in the stem tissue of green plants by using a tissue-specific promoter patent-application April 2005
Sequence-determined DNA fragments and corresponding polypeptides encoded thereby patent-application May 2006
Transcription Factors That Enhance Traits in Plant Organs patent-application June 2010
Compositions and Methods for Regulating Polysaccharides of a Plant Cell patent-application July 2010
Plants Having Enhanced Yield-Related Traits and a Method for Making the Same patent-application August 2010
Engineering of plants with improved properties as biofuels feedstocks by vessel-specific complementation of xylan biosynthesis mutants journal January 2012
Multiple Repeats of a Promoter Segment Causes Transcription Factor Autoregulation in Red Apples journal January 2009
Biosynthesis of Pectin journal April 2010
ARAD proteins associated with pectic Arabinan biosynthesis form complexes when transiently overexpressed in planta journal January 2012
Identification of a Xylogalacturonan Xylosyltransferase Involved in Pectin Biosynthesis in Arabidopsis journal May 2008
NAC Transcription Factors, NST1 and NST3, Are Key Regulators of the Formation of Secondary Walls in Woody Tissues of Arabidopsis journal January 2007
Functional identification of an Arabidopsis pectin biosynthetic homogalacturonan galacturonosyltransferase journal March 2006

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