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Title: Modified cellulose synthase gene from 'Arabidopsis thaliana' confers herbicide resistance to plants

Abstract

Cellulose synthase ('CS'), a key enzyme in the biosynthesis of cellulose in plants is inhibited by herbicides comprising thiazolidinones such as 5-tert-butyl-carbamoyloxy-3-(3-trifluromethyl) phenyl-4-thiazolidinone (TZ), isoxaben and 2,6-dichlorobenzonitrile (DCB). Two mutant genes encoding isoxaben and TZ-resistant cellulose synthase have been isolated from isoxaben and TZ-resistant Arabidopsis thaliana mutants. When compared with the gene coding for isoxaben or TZ-sensitive cellulose synthase, one of the resistant CS genes contains a point mutation, wherein glycine residue 998 is replaced by an aspartic acid. The other resistant mutation is due to a threonine to isoleucine change at amino acid residue 942. The mutant CS gene can be used to impart herbicide resistance to a plant; thereby permitting the utilization of the herbicide as a single application at a concentration which ensures the complete or substantially complete killing of weeds, while leaving the transgenic crop plant essentially undamaged.

Inventors:
;
Publication Date:
Research Org.:
Carnegie Institute of Washington (US)
Sponsoring Org.:
US Department of Energy (US)
OSTI Identifier:
808234
Patent Number(s):
PATENTS-US-A9686234
Application Number:
9-686,234; TRN: US200306%%346
Assignee:
CHO
DOE Contract Number:  
FG02-94ER20133
Resource Type:
Patent Application
Resource Relation:
Patent File Date: 2000 Oct 11; Other Information: PBD: 11 Oct 2000
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; AMINO ACIDS; ARABIDOPSIS; ASPARTIC ACID; BIOSYNTHESIS; CELLULOSE; CROPS; ENZYMES; GENE MUTATIONS; GENES; HERBICIDES; MUTANTS; MUTATIONS; THREONINE

Citation Formats

Somerville, Chris R, and Scieble, Wolf. Modified cellulose synthase gene from 'Arabidopsis thaliana' confers herbicide resistance to plants. United States: N. p., 2000. Web.
Somerville, Chris R, & Scieble, Wolf. Modified cellulose synthase gene from 'Arabidopsis thaliana' confers herbicide resistance to plants. United States.
Somerville, Chris R, and Scieble, Wolf. Wed . "Modified cellulose synthase gene from 'Arabidopsis thaliana' confers herbicide resistance to plants". United States. https://www.osti.gov/servlets/purl/808234.
@article{osti_808234,
title = {Modified cellulose synthase gene from 'Arabidopsis thaliana' confers herbicide resistance to plants},
author = {Somerville, Chris R and Scieble, Wolf},
abstractNote = {Cellulose synthase ('CS'), a key enzyme in the biosynthesis of cellulose in plants is inhibited by herbicides comprising thiazolidinones such as 5-tert-butyl-carbamoyloxy-3-(3-trifluromethyl) phenyl-4-thiazolidinone (TZ), isoxaben and 2,6-dichlorobenzonitrile (DCB). Two mutant genes encoding isoxaben and TZ-resistant cellulose synthase have been isolated from isoxaben and TZ-resistant Arabidopsis thaliana mutants. When compared with the gene coding for isoxaben or TZ-sensitive cellulose synthase, one of the resistant CS genes contains a point mutation, wherein glycine residue 998 is replaced by an aspartic acid. The other resistant mutation is due to a threonine to isoleucine change at amino acid residue 942. The mutant CS gene can be used to impart herbicide resistance to a plant; thereby permitting the utilization of the herbicide as a single application at a concentration which ensures the complete or substantially complete killing of weeds, while leaving the transgenic crop plant essentially undamaged.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2000},
month = {10}
}

Patent Application:

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