Title: Processing of cellulosic material by a cellulase-containing cell-free fermentate produced from cellulase-producing bacteria, ATCC 55702

Abstract

Bacteria which produce large amounts of a cellulase-containing cell-free fermentate, have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase degrading bacterium ATCC 55702, which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic materials.

Inventors:

Dees, H Craig ^[1]

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1. Lenoir City, TN

Issue Date:

Thu Jan 01 00:00:00 EST 1998

Research Org.:

Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

OSTI Identifier:

871752

Patent Number(s):

5789227

Assignee:

Lockheed Martin Energy Systems, Inc. (Oak Ridge, TN)

Patent Classifications (CPCs):

C - CHEMISTRY C11 - ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES C11D - DETERGENT COMPOSITIONS

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C11 - ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES C11D - DETERGENT COMPOSITIONS

C11D3/38645 - {containing cellulase}

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/01 - Preparation of mutants without inserting foreign genetic material therein
C12N9/2437 - {Cellulases

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DOE Contract Number:  

AC05-84OR21400

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

processing; cellulosic; material; cellulase-containing; cell-free; fermentate; produced; cellulase-producing; bacteria; atcc; 55702; produce; amounts; identified; original; bacterium; 55703; genetically; altered; nitrosoguanidine; mnng; treatment; enhanced; cellulase; degrading; replicate; plating; improved; characteristics; qualities; degradation; materials; cellulase-containing cell-free; improved characteristics; original bacterium; cellulosic material; enhanced cellulase; replicate plating; cellulosic materials; genetically altered; cell-free fermentate; fermentate produced; producing bacteria; /435/