Method of producing a cellulase-containing cell-free fermentate produced from microorganism ATCC 55702
Abstract
Bacteria which produce large amounts of cellulose-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques. 5 figs.
- Inventors:
- Issue Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE, Washington, DC (United States)
- OSTI Identifier:
- 644362
- Patent Number(s):
- 5756337
- Application Number:
- PAN: 8-729,816
- Assignee:
- Lockheed Martin Energy Systems, Inc., Oak Ridge, TN (United States)
- DOE Contract Number:
- AC05-84OR21400
- Resource Type:
- Patent
- Resource Relation:
- Other Information: PBD: 26 May 1998
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; CELLULOSE; CELLULASE; BACTERIA; FERMENTATION; GENETIC ENGINEERING
Citation Formats
Dees, H C. Method of producing a cellulase-containing cell-free fermentate produced from microorganism ATCC 55702. United States: N. p., 1998.
Web.
Dees, H C. Method of producing a cellulase-containing cell-free fermentate produced from microorganism ATCC 55702. United States.
Dees, H C. Tue .
"Method of producing a cellulase-containing cell-free fermentate produced from microorganism ATCC 55702". United States.
@article{osti_644362,
title = {Method of producing a cellulase-containing cell-free fermentate produced from microorganism ATCC 55702},
author = {Dees, H C},
abstractNote = {Bacteria which produce large amounts of cellulose-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques. 5 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue May 26 00:00:00 EDT 1998},
month = {Tue May 26 00:00:00 EDT 1998}
}
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