DNA fragment sizing and sorting by laser-induced fluorescence
Abstract
A method is provided for sizing DNA fragments using high speed detection systems, such as flow cytometry to determine unique characteristics of DNA pieces from a sample. In one characterization the DNA piece is fragmented at preselected sites to produce a plurality of DNA fragments. The DNA piece or the resulting DNA fragments are treated with a dye effective to stain stoichiometrically the DNA piece or the DNA fragments. The fluorescence from the dye in the stained fragments is then examined to generate an output functionally related to the number of nucleotides in each one of the DNA fragments. In one embodiment, the intensity of the fluorescence emissions from each fragment is linearly related to the fragment length. The distribution of DNA fragment sizes forms a characterization of the DNA piece for use in forensic and research applications.
- Inventors:
-
- Angier, NC
- Los Alamos, NM
- Issue Date:
- Research Org.:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- OSTI Identifier:
- 870612
- Patent Number(s):
- 5558998
- Assignee:
- Regents of Univ. of California (Alameda, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- W-7405-ENG-36
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- dna; fragment; sizing; sorting; laser-induced; fluorescence; method; provided; fragments; speed; detection; systems; flow; cytometry; determine; unique; characteristics; pieces; sample; characterization; piece; fragmented; preselected; sites; produce; plurality; resulting; treated; dye; effective; stain; stoichiometrically; stained; examined; generate; output; functionally; related; nucleotides; embodiment; intensity; emissions; linearly; length; distribution; sizes; forms; forensic; applications; laser-induced fluorescence; detection systems; functionally related; dna fragments; fluorescence emission; flow cytometry; dna fragment; unique characteristics; induced fluorescence; fluorescence emissions; linearly related; fragment sizing; unique characteristic; /435/436/
Citation Formats
Hammond, Mark L, Jett, James H, Keller, Richard A, Marrone, Babetta L, and Martin, John C. DNA fragment sizing and sorting by laser-induced fluorescence. United States: N. p., 1996.
Web.
Hammond, Mark L, Jett, James H, Keller, Richard A, Marrone, Babetta L, & Martin, John C. DNA fragment sizing and sorting by laser-induced fluorescence. United States.
Hammond, Mark L, Jett, James H, Keller, Richard A, Marrone, Babetta L, and Martin, John C. Mon .
"DNA fragment sizing and sorting by laser-induced fluorescence". United States. https://www.osti.gov/servlets/purl/870612.
@article{osti_870612,
title = {DNA fragment sizing and sorting by laser-induced fluorescence},
author = {Hammond, Mark L and Jett, James H and Keller, Richard A and Marrone, Babetta L and Martin, John C},
abstractNote = {A method is provided for sizing DNA fragments using high speed detection systems, such as flow cytometry to determine unique characteristics of DNA pieces from a sample. In one characterization the DNA piece is fragmented at preselected sites to produce a plurality of DNA fragments. The DNA piece or the resulting DNA fragments are treated with a dye effective to stain stoichiometrically the DNA piece or the DNA fragments. The fluorescence from the dye in the stained fragments is then examined to generate an output functionally related to the number of nucleotides in each one of the DNA fragments. In one embodiment, the intensity of the fluorescence emissions from each fragment is linearly related to the fragment length. The distribution of DNA fragment sizes forms a characterization of the DNA piece for use in forensic and research applications.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 1996},
month = {Mon Jan 01 00:00:00 EST 1996}
}
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