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Title: Laser-induced differential normalized fluorescence method for cancer diagnosis

Abstract

An apparatus and method for cancer diagnosis are disclosed. The diagnostic method includes the steps of irradiating a tissue sample with monochromatic excitation light, producing a laser-induced fluorescence spectrum from emission radiation generated by interaction of the excitation light with the tissue sample, and dividing the intensity at each wavelength of the laser-induced fluorescence spectrum by the integrated area under the laser-induced fluorescence spectrum to produce a normalized spectrum. A mathematical difference between the normalized spectrum and an average value of a reference set of normalized spectra which correspond to normal tissues is calculated, which provides for amplifying small changes in weak signals from malignant tissues for improved analysis. The calculated differential normalized spectrum is correlated to a specific condition of a tissue sample.

Inventors:
 [1];  [1];  [1]
  1. Knoxville, TN
Issue Date:
Research Org.:
LOCKHEED MARTIN ENRGY SYST INC
OSTI Identifier:
870708
Patent Number(s):
5579773
Assignee:
Martin Marietta Energy Systems, Inc. (Oak Ridge, TN)
DOE Contract Number:  
AC05-84OR21400
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
laser-induced; differential; normalized; fluorescence; method; cancer; diagnosis; apparatus; disclosed; diagnostic; steps; irradiating; tissue; sample; monochromatic; excitation; light; producing; spectrum; emission; radiation; generated; interaction; dividing; intensity; wavelength; integrated; produce; mathematical; difference; average; value; reference; set; spectra; correspond; normal; tissues; calculated; provides; amplifying; changes; weak; signals; malignant; improved; analysis; correlated; specific; condition; laser-induced fluorescence; tissue sample; diagnostic method; radiation generated; excitation light; induced fluorescence; fluorescence spectrum; normal tissue; fluorescence method; cancer diagnosis; malignant tissue; /600/

Citation Formats

Vo-Dinh, Tuan, Panjehpour, Masoud, and Overholt, Bergein F. Laser-induced differential normalized fluorescence method for cancer diagnosis. United States: N. p., 1996. Web.
Vo-Dinh, Tuan, Panjehpour, Masoud, & Overholt, Bergein F. Laser-induced differential normalized fluorescence method for cancer diagnosis. United States.
Vo-Dinh, Tuan, Panjehpour, Masoud, and Overholt, Bergein F. Mon . "Laser-induced differential normalized fluorescence method for cancer diagnosis". United States. https://www.osti.gov/servlets/purl/870708.
@article{osti_870708,
title = {Laser-induced differential normalized fluorescence method for cancer diagnosis},
author = {Vo-Dinh, Tuan and Panjehpour, Masoud and Overholt, Bergein F},
abstractNote = {An apparatus and method for cancer diagnosis are disclosed. The diagnostic method includes the steps of irradiating a tissue sample with monochromatic excitation light, producing a laser-induced fluorescence spectrum from emission radiation generated by interaction of the excitation light with the tissue sample, and dividing the intensity at each wavelength of the laser-induced fluorescence spectrum by the integrated area under the laser-induced fluorescence spectrum to produce a normalized spectrum. A mathematical difference between the normalized spectrum and an average value of a reference set of normalized spectra which correspond to normal tissues is calculated, which provides for amplifying small changes in weak signals from malignant tissues for improved analysis. The calculated differential normalized spectrum is correlated to a specific condition of a tissue sample.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1996},
month = {1}
}

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