Means and method for capillary zone electrophoresis with laser-induced indirect fluorescence detection

Yeung, Edward S.; Kuhr, Werner G.

Title: Means and method for capillary zone electrophoresis with laser-induced indirect fluorescence detection

Patent · Tue Feb 20 00:00:00 EST 1996

OSTI ID:1176695

Yeung, Edward S.; Kuhr, Werner G.

A means and method for capillary zone electrphoresis with laser-induced indirect fluorescence detection. A detector is positioned on the capillary tube of a capillary zone electrophoresis system. The detector includes a laser which generates a laser beam which is imposed upon a small portion of the capillary tube. Fluorescence of the elutant electromigrating through the capillary tube is indirectly detected and recorded.

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Research Organization:: Ames Laboratory (AMES), Ames, IA (United States); Iowa State Univ., Ames, IA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: W-7405-ENG-82

Assignee:: Iowa State University Research Foundation, Inc. (Ames, IA)

Patent Number(s):: RE35157

Application Number:: 08/045,630; 5,006,210

OSTI ID:: 1176695

Resource Relation:: Patent File Date: 1993 Apr 09

Country of Publication:: United States

Language:: English

References (13)

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Simultaneous laser-based refractive index and absorbance determinations within micrometer diameter capillary tubes Bornhop, Darryl J.; Dovichi, Norman J. Analytical Chemistry, Vol. 59, Issue 13 https://doi.org/10.1021/ac00140a011	journal	July 1987
High-resolution separations based on electrophoresis and electroosmosis Jorgenson, James W.; Lukacs, Krynn DeArman Journal of Chromatography A, Vol. 218 https://doi.org/10.1016/S0021-9673(00)82057-9	journal	November 1981
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Carrier-free zone electrophoresis, displacement electrophoresis and isoelectric focusing in a high-performance electrophoresis apparatus Hjertén, Stellan; Elenbring, Karin; Kilár, Ferenc Journal of Chromatography A, Vol. 403 https://doi.org/10.1016/S0021-9673(00)96340-4	journal	January 1987
Electrokinetic resolution of amino acid enantiomers with copper(II)-aspartame support electrolyte Gozel, Philippe.; Gassmann, Ernst.; Michelsen, Hope. Analytical Chemistry, Vol. 59, Issue 1 https://doi.org/10.1021/ac00128a009	journal	January 1987
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Analysis of B6 Vitamers by Micellar Electrokinetic Capillary Chromatography with Laser-Excited Fluorescence Detection Burton, D. E.; Sepaniak, M. J.; Maskarinec, M. P. Journal of Chromatographic Science, Vol. 24, Issue 8 https://doi.org/10.1093/chromsci/24.8.347	journal	August 1986
Detection method for ion chromatography based on double-beam laser-excited indirect fluorometry Mho, Sun Il.; Yeung, Edward S. Analytical Chemistry, Vol. 57, Issue 12 https://doi.org/10.1021/ac00289a018	journal	October 1985
Zone electrophoresis in open-tubular glass capillaries Jorgenson, James W.; Lukacs, Krynn DeArman. Analytical Chemistry, Vol. 53, Issue 8 https://doi.org/10.1021/ac00231a037	journal	July 1981
Determination of methotrexate and its major metabolite, 7-hydroxymethotrexate, using capillary zone electrophoresis and laser-induced fluorescence detection Roach, Mark C.; Gozel, Philippe; Zare, Richard N. Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 426 https://doi.org/10.1016/S0378-4347(00)81934-9	journal	January 1988
Nanoliter volume sequential differential concentration gradient detector Pawliszyn, Janusz. Analytical Chemistry, Vol. 60, Issue 24 https://doi.org/10.1021/ac00175a031	journal	December 1988

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