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Title: Ultrasonic analyte concentration and application in flow cytometry

Abstract

The present invention includes an apparatus and corresponding method for concentrating analytes within a fluid flowing through a tube using acoustic radiation pressure. The apparatus includes a function generator that outputs a radio frequency electrical signal to a transducer that transforms the radio frequency electric signal to an acoustic signal and couples the acoustic signal to the tube. The acoustic signal is converted within the tube to acoustic pressure that concentrates the analytes within the fluid.

Inventors:
; ; ; ; ; ;
Issue Date:
Research Org.:
Triad National Security, LLC, Los Alamos, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1632452
Patent Number(s):
10537831
Application Number:
14/729,244
Assignee:
Triad National Security, LLC (Los Alamos, NM)
Patent Classifications (CPCs):
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01D - SEPARATION
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
89233218CNA000001
Resource Type:
Patent
Resource Relation:
Patent File Date: 06/03/2015
Country of Publication:
United States
Language:
English
Subject:
73 NUCLEAR PHYSICS AND RADIATION PHYSICS; 71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS

Citation Formats

Kaduchak, Gregory, Goddard, Greg, Salzman, Gary, Sinha, Dipen, Martin, John C., Kwiatkowski, Christopher, and Graves, Steven. Ultrasonic analyte concentration and application in flow cytometry. United States: N. p., 2020. Web.
Kaduchak, Gregory, Goddard, Greg, Salzman, Gary, Sinha, Dipen, Martin, John C., Kwiatkowski, Christopher, & Graves, Steven. Ultrasonic analyte concentration and application in flow cytometry. United States.
Kaduchak, Gregory, Goddard, Greg, Salzman, Gary, Sinha, Dipen, Martin, John C., Kwiatkowski, Christopher, and Graves, Steven. Tue . "Ultrasonic analyte concentration and application in flow cytometry". United States. https://www.osti.gov/servlets/purl/1632452.
@article{osti_1632452,
title = {Ultrasonic analyte concentration and application in flow cytometry},
author = {Kaduchak, Gregory and Goddard, Greg and Salzman, Gary and Sinha, Dipen and Martin, John C. and Kwiatkowski, Christopher and Graves, Steven},
abstractNote = {The present invention includes an apparatus and corresponding method for concentrating analytes within a fluid flowing through a tube using acoustic radiation pressure. The apparatus includes a function generator that outputs a radio frequency electrical signal to a transducer that transforms the radio frequency electric signal to an acoustic signal and couples the acoustic signal to the tube. The acoustic signal is converted within the tube to acoustic pressure that concentrates the analytes within the fluid.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 21 00:00:00 EST 2020},
month = {Tue Jan 21 00:00:00 EST 2020}
}

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Experimental study of streaming flows associated with ultrasonic levitators
journal, November 1994


Methods, compositions, and automated systems for separating rare cells from fluid samples
patent, September 2005


A numerically stable global matrix method for cylindrically layered shells excited by ring forces
journal, June 1994


Compact acoustic levitation device for studies in fluid dynamics and material science in the laboratory and microgravity
journal, November 1985


Method of non-contact micromanipulation using ultrasound
patent, November 1998


Resonator frequency shift due to ultrasonically induced microparticle migration in an aqueous suspension: Observations and model for the maximum frequency shift
journal, June 1998

  • Kwiatkowski, Christopher S.; Marston, Philip L.
  • The Journal of the Acoustical Society of America, Vol. 103, Issue 6, p. 3290-3300
  • https://doi.org/10.1121/1.423080

Method and apparatus for separating particles
patent, July 1993


Particle separation using acoustic radiation force and elecrostatic force
journal, April 1996

  • Yasuda, Kenji; Umemura, Shin‐ichiro; Takeda, Kazuo
  • The Journal of the Acoustical Society of America, Vol. 99, Issue 4, p. 1965-1970
  • https://doi.org/10.1121/1.415382

SPART analyzer: its application to aerodynamic size distribution measurement
journal, January 1979


Modelling in the design of a flow-through ultrasonic separator
journal, March 2000


Manipulation of Fluid Objects with Acoustic Radiation Pressure
journal, January 2006


Device and method for separation
patent, August 2005


Virus removal in a membrane separation process
journal, May 1998


Non-contact micromanipulation method and apparatus
patent, May 2000


Mathematical modeling of the movement of suspended particles subjected to acoustic and flow fields
journal, June 2005


Kits for systems and methods using acoustic radiation pressure
patent, October 2016


Cavitation bubble-driven cell and particle behavior in an ultrasound standing wave
journal, January 2005

  • Kuznetsova, Larisa A.; Khanna, Sanjay; Amso, Nazar N.
  • The Journal of the Acoustical Society of America, Vol. 117, Issue 1, p. 104-112
  • https://doi.org/10.1121/1.1835503

Time-resolved flow cytometry for the measurement of lanthanide chelate fluorescence: I. Concept and theoretical evaluation
journal, July 1994


Time-resolved flow cytometry for the measurement of lanthanide chelate fluorescence: II. Instrument design and experimental results
journal, July 1994


Design, modeling and characterization of microfluidic devices for ultrasonic manipulation
journal, February 2007


Acoustic filter for separating and recycling suspended particles
patent, May 1997


Acoustically driven collection of suspended particles within porous media
journal, March 1997


Particle Quantifying Systems and Methods Using Acoustic Radiation Pressure
patent-application, February 2009


Force field particle filter, combining ultrasound standing waves and laminar flow
journal, May 2001