Methods of expressing and detecting activity of expansin in plant cells
Abstract
A method of expressing heterologous expansin in a plant cell is provided where a nucleic acid molecule encoding expansin is introduced into the plant cell and in an embodiment is operably linked to a promoter preferentially expressing in the seed tissue of the plant, and in another embodiment is linked to a promoter preferentially expressing in the embryo tissue of the seed. An embodiment provides the nucleic acid molecule is operably linked to a second nucleic acid molecule that directs expression to the endoplasmic reticulum, vacuole or cell wall. Plants and plant parts expressing expansin are provided. An assay for detection of expansin activity is also provided.
- Inventors:
- Issue Date:
- Research Org.:
- Arkansas State University, State University, AR (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1398984
- Patent Number(s):
- 9783817
- Application Number:
- 14/196,412
- Assignee:
- Arkansas State University
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- FG36-08GO88025
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2014 Mar 04
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Hood, Elizabeth E., and Yoon, Sangwoong. Methods of expressing and detecting activity of expansin in plant cells. United States: N. p., 2017.
Web.
Hood, Elizabeth E., & Yoon, Sangwoong. Methods of expressing and detecting activity of expansin in plant cells. United States.
Hood, Elizabeth E., and Yoon, Sangwoong. Tue .
"Methods of expressing and detecting activity of expansin in plant cells". United States. https://www.osti.gov/servlets/purl/1398984.
@article{osti_1398984,
title = {Methods of expressing and detecting activity of expansin in plant cells},
author = {Hood, Elizabeth E. and Yoon, Sangwoong},
abstractNote = {A method of expressing heterologous expansin in a plant cell is provided where a nucleic acid molecule encoding expansin is introduced into the plant cell and in an embodiment is operably linked to a promoter preferentially expressing in the seed tissue of the plant, and in another embodiment is linked to a promoter preferentially expressing in the embryo tissue of the seed. An embodiment provides the nucleic acid molecule is operably linked to a second nucleic acid molecule that directs expression to the endoplasmic reticulum, vacuole or cell wall. Plants and plant parts expressing expansin are provided. An assay for detection of expansin activity is also provided.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Oct 10 00:00:00 EDT 2017},
month = {Tue Oct 10 00:00:00 EDT 2017}
}
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