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Title: Consolidated pretreatment and hydrolysis of plant biomass expressing cell wall degrading enzymes

Abstract

Methods for consolidated pretreatment and hydrolysis of genetically engineered plants expressing cell wall degrading enzymes are provided. Expression cassettes and vectors for making transgenic plants are described. Plants engineered to express one or more cell wall degrading enzymes using expression cassettes and vectors of the invention are also provided.

Inventors:
; ;
Issue Date:
Research Org.:
AGRIVIDA, INC., Medford, MA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1460546
Patent Number(s):
10006038
Application Number:
14/961,426
Assignee:
AGRIVIDA, INC. (Medford, MA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
DOE Contract Number:  
AR0000042
Resource Type:
Patent
Resource Relation:
Patent File Date: 2015 Dec 07
Country of Publication:
United States
Language:
English

Citation Formats

Raab, R. Michael, Zhang, Dongcheng, and Bougri, Oleg. Consolidated pretreatment and hydrolysis of plant biomass expressing cell wall degrading enzymes. United States: N. p., 2018. Web.
Raab, R. Michael, Zhang, Dongcheng, & Bougri, Oleg. Consolidated pretreatment and hydrolysis of plant biomass expressing cell wall degrading enzymes. United States.
Raab, R. Michael, Zhang, Dongcheng, and Bougri, Oleg. Tue . "Consolidated pretreatment and hydrolysis of plant biomass expressing cell wall degrading enzymes". United States. https://www.osti.gov/servlets/purl/1460546.
@article{osti_1460546,
title = {Consolidated pretreatment and hydrolysis of plant biomass expressing cell wall degrading enzymes},
author = {Raab, R. Michael and Zhang, Dongcheng and Bougri, Oleg},
abstractNote = {Methods for consolidated pretreatment and hydrolysis of genetically engineered plants expressing cell wall degrading enzymes are provided. Expression cassettes and vectors for making transgenic plants are described. Plants engineered to express one or more cell wall degrading enzymes using expression cassettes and vectors of the invention are also provided.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {6}
}

Patent:

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Works referenced in this record:

Ribonucleotide reductase in the archaeon Pyrococcus furiosus: A critical enzyme in the evolution of DNA genomes?
journal, January 1997


Plant genetic engineering for biofuel production: towards affordable cellulosic ethanol
journal, June 2008


The use of nuclear-encoded sequences to direct the light-regulated synthesis and transport of a foreign protein into plant chloroplasts
journal, January 1985


Production of ethanol from wood hydrolyzate by yeasts
journal, May 2000


Semisynthesis of cytotoxic proteins using a modified protein splicing element
journal, November 1998


Stable expression of a thermostable xylanase of Clostridium thermocellum in cultured tobacco cells
journal, January 2003


The Thy Pol-2 intein of Thermococcus hydrothermalis is an isoschizomer of PI-TliI and PI-TfuII endonucleases
journal, November 2000


A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures
journal, July 1962


Dual targeting of xylanase to chloroplasts and peroxisomes as a means to increase protein accumulation in plant cells
journal, November 2005


The in Vitro Ligation of Bacterially Expressed Proteins Using an Intein from Methanobacterium thermoautotrophicum
journal, February 1999


Genomic sequence of a 12S seed storage protein from oilseed rape ( Brassica napus c.v. jet neuf)
journal, January 1989


Corn as a production system for human and animal vaccines
journal, January 2003


Invariant Asp-1122 and Asp-1124 Are Essential Residues for Polymerization Catalysis of Family D DNA Polymerase from Pyrococcus horikoshii
journal, April 2001


Gene transfer to plants by diverse species of bacteria
journal, February 2005


Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: A review
journal, July 2010


Improvement of ethanol production from starch by recombinant yeast through manipulation of environmental factors
journal, October 2002


Characterization of DNA polymerase from Pyrococcus sp. strain KOD1 and its application to PCR.
journal, January 1997


Improving Enzymes for Biomass Conversion: A Basic Research Perspective
journal, January 2010


Purification of Proteins Fused to Either the Amino or Carboxy Terminus of the Mycobacterium xenopi Gyrase A Intein
journal, July 1999


Production of a recombinant xylanase in plants and its potential for pulp biobleaching applications
journal, June 2008


Transformation of Maize Cells and Regeneration of Fertile Transgenic Plants.
journal, July 1990


Compilation and analysis of intein sequences
journal, March 1997


Cloning, expression, and characterization of DNA polymerase I from the hyperthermophilic archaea Thermococcus fumicolans
journal, August 2000


Metazoan cellulase genes from termites: intron/exon structures and sites of expression
journal, October 1999


Expression Enhancement of a Rice Polyubiquitin Gene Promoter
journal, January 2006


Microwave-assisted pretreatment of woody biomass with ammonium molybdate activated by H2O2
journal, February 2011


A genetic system yields self-cleaving inteins for bioseparations
journal, September 1999


In planta expression of A. cellulolyticus Cel5A endocellulase reduces cell wall recalcitrance in tobacco and maize
journal, January 2011


Complete nucleotide sequence of alfalfa mosaic virus RNA 4
journal, January 1980


Breaking up is hard to do
journal, January 1998


Optimized Single-Step Affinity Purification with a Self-Cleaving Intein Applied to Human Acidic Fibroblast Growth Factor
journal, December 2000


NMR Observation of Selected Segments in a Larger Protein:  Central-Segment Isotope Labeling through Intein-Mediated Ligation
journal, December 1999


Modulation of Protein Splicing of the Saccharomyces cerevisiae Vacuolar Membrane ATPase Intein
journal, April 1998


Genome sequence of Halobacterium species NRC-1
journal, October 2000


Protein Splicing: Characterization of the Aminosuccinimide Residue at the Carboxyl Terminus of the Excised Intervening Sequence
journal, August 1995


Dry-Grind Process for Fuel Ethanol by Continuous Fermentation and Stripping
journal, August 2000


High-yield production of a human therapeutic protein in tobacco chloroplasts
journal, March 2000


Coordinated development of leading biomass pretreatment technologies
journal, December 2005


A short domain of the plant vacuolar protein phytohemagglutinin targets invertase to the yeast vacuole.
journal, June 1990


Herbicide resistance from a divided EPSPS protein: the split Synechocystis DnaE intein as an in vivo affinity domain
journal, January 2001


The intein of the Thermoplasma A-ATPase A subunit: Structure, evolution and expression in E. coli
journal, January 2001


Inteins of Thermococcus fumicolans DNA Polymerase Are Endonucleases with Distinct Enzymatic Behaviors
journal, January 2000


Fertile transgenic rice plants regenerated from transformed protoplasts
journal, March 1989


Lightning strikes twice: Intron-intein coincidence
journal, February 1998


The complete genome of the hyperthermophilic bacterium Aquifex aeolicus
journal, March 1998


Enhanced turnover of transitory starch by expression of up-regulated ADP-glucose pyrophosphorylases in Arabidopsis thaliana
journal, January 2006


Synthesis, processing and export of cytoplasmic endo‐β‐1,4‐xylanase from barley aleurone during germination
journal, April 2001


Ionic liquid pretreatment of cellulosic biomass: Enzymatic hydrolysis and ionic liquid recycle
journal, December 2010


Plant cell wall reconstruction toward improved lignocellulosic production and processability
journal, February 2010


InBase: the Intein Database
journal, January 2002


Transformation of Poplar by Agrobacterium tumefaciens
journal, June 1986


Statistical Modeling, Phylogenetic Analysis and Structure Prediction of a Protein Splicing Domain Common to Inteins and Hedgehog Proteins
journal, January 1997


Characterization of two intein homing endonucleases encoded in the DNA polymerase gene of Pyrococcus kodakaraensis strain KOD1
journal, October 1998


Enhancement of growth and cellulose accumulation by overexpression of xyloglucanase in poplar
journal, March 2004


Green chemistry yields a better plastic
journal, October 1999


The tomato polygalacturonase gene and ripening-specific expression in transgenic plants
journal, January 1988


Secretion of a heat-stable fungalβ-glucanase from transgenic, suspension-cultured barley cells
journal, March 1995


Protein Splicing in the Absence of an Intein Penultimate Histidine
journal, April 2000


Protein trans-Splicing To Produce Herbicide-Resistant Acetolactate Synthase
journal, March 2001


Biological pretreatment of wheat straw by Phanerochaete chrysosporium supplemented with inorganic salts
journal, February 2011


Novel structure of the recA locus of Mycobacterium tuberculosis implies processing of the gene product.
journal, January 1991


Pretreatment of switchgrass for sugar production with the combination of sodium hydroxide and lime
journal, February 2011


Features of promising technologies for pretreatment of lignocellulosic biomass
journal, April 2005


Commercial cellulosic ethanol: The role of plant-expressed enzymes
journal, May 2009


Transgenic barley expressing a fungal xylanase gene in the endosperm of the developing grains
journal, January 2000


Biobased Industrial Products: Bioprocess Engineering When Cost Really Counts
journal, October 1999


The starch-related R1 protein is an α-glucan, water dikinase
journal, May 2002


A review of the production of ethanol from softwood
journal, July 2002


Protein splicing elements: inteins and exteins — a definition of terms and recommended nomenclature
journal, January 1994


Identification of an Unusual Intein in Chloroplast ClpP Protease of Chlamydomonas eugametos
journal, May 1997


The Mycobacterium xenopi GyrA protein splicing element: characterization of a minimal intein.
journal, January 1997


Metabolic Engineering of Propanediol Pathways
journal, February 1998


Protein transport into and within chloroplasts
journal, February 1990


Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics.
journal, November 1997


Heterologous Acidothermus cellulolyticus 1,4-β-endoglucanase E1 produced within the corn biomass converts corn stover into glucose
journal, April 2007


Fermentation of lignocellulosic hydrolysates for ethanol production
journal, April 1996


Characterization of the Escherichia coli RNA 3′-Terminal Phosphate Cyclase and Its ς 54 -Regulated Operon
journal, September 1998


Segmental Isotope Labeling for Protein NMR Using Peptide Splicing
journal, June 1998


Protein trans-splicing in transgenic plant chloroplast: Reconstruction of herbicide resistance from split genes
journal, April 2003


Enzymatic deconstruction of xylan for biofuel production
journal, February 2009


In vitro protein splicing of purified precursor and the identification of a branched intermediate
journal, December 1993


A proposed mechanism for the self-splicing of proteins.
journal, November 1994


Protein splicing ? the lengths some proteins will go to
journal, January 1995


The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum
journal, September 2000


The mechanism of protein splicing and its modulation by mutation.
journal, October 1996


Biorefineries:  Current Status, Challenges, and Future Direction
journal, July 2006


Cloning of the xynB Gene fromDictyoglomus thermophilum Rt46B.1 and Action of the Gene Product on Kraft Pulp
journal, May 1998


Invasion of a multitude of genetic niches by mobile endonuclease genes
journal, April 2000


Control of protein splicing by intein fragment reassembly
journal, February 1998


Cloning and characterisation of a thermostable α-DNA polymerase from the hyperthermophilic archaeon Thermococcus sp. TY
journal, December 1997


In vitro splicing of erythropoietin by the Mycobacterium tuberculosis RecA intein without substituting amino acids at the splice junctions
journal, January 2003


Structural features affecting biomass enzymatic digestibility
journal, June 2008


Protein Splicing:  Evidence for an N−O Acyl Rearrangement as the Initial Step in the Splicing Process
journal, January 1996


Intervening sequences in an Archaea DNA polymerase gene.
journal, June 1992


Intein-mediated assembly of a functional β-glucuronidase in transgenic plants
journal, March 2003


Autohydrolysis of plant xylans by apoplastic expression of thermophilic bacterial endo-xylanases
journal, April 2010


Reactivity of the Cysteine Residues in the Protein Splicing Active Center of the Mycobacterium tuberculosis RecA Intein
journal, March 2000


Improved segmental isotope labeling of proteins and application to a larger protein
journal, January 1999


Improved protocols for transformation of indica rice mediated by Agrobacterium tumefaciens
journal, May 2006


Production of Correctly Processed Human Serum Albumin in Transgenic Plants
journal, March 1990


Expression of biologically active Acidothermus cellulolyticus endoglucanase in transgenic maize plants
journal, November 2006


Towards a comparative anatomy of N-terminal topogenic protein sequences
journal, May 1986


Pretreatment of woody biomass for biofuel production: energy efficiency, technologies, and recalcitrance
journal, May 2010


Bioconversion of starch into ethanol by a recombinant Saccharomyces cerevisiae strain YPG-AB
journal, May 2002


Modular organization of inteins and C-terminal autocatalytic domains
journal, January 1998


PLANT TRANSFORMATION: Problems and Strategies for Practical Application
journal, June 1997


pBINPLUS/ARS: an improved plant transformation vector based on pBINPLUS
journal, May 2008