Method of increasing expression of nucleic acid molecules in plants using multiple transcription units

Howard, John; Egelkrout, Erin; Hayden, Celine

Title: Method of increasing expression of nucleic acid molecules in plants using multiple transcription units

Abstract

A method of increasing expression of a product encoded by a nucleic acid molecule is provided where in one embodiment multiple plant transcription units comprising a promoter and the nucleic acid molecule are provided. In another embodiment multiple plant transcription units are provided where each promoter is different from the other. In an embodiment the multiple plant transcription units may comprise two, three, four or more plant transcription units. In another embodiment the promoter may be selected from an embryo promoter. Another embodiment provides the promoter may be a globulin promoter. A further embodiment provides the product encoded may be selected from hepatitis b, aprotinin, or a cellulase. Still further embodiments provide the product may be selected from the cellulases E1 and CBH1.

Inventors:: Howard, John; Egelkrout, Erin; Hayden, Celine

Issue Date:: 2019-06-04

Research Org.:: Applied Biotechnology Inst., Inc., San Luis Obispo, CA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1568424

Patent Number(s):: 10308948

Application Number:: 13/654,673

Assignee:: Applied Biotechnology Institute, Inc. (San Luis Obispo, CA)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/8258 - {for the production of oral vaccines
C12N15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
C12N15/8234 - {Seed-specific, e.g. embryo, endosperm}
C12N15/8216 - {Methods for controlling, regulating or enhancing expression of transgenes in plant cells}
C12N15/8257 - {for the production of primary gene products, e.g. pharmaceutical products, interferon}

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DOE Contract Number:: FG36-08GO88025

Resource Type:: Patent

Resource Relation:: Patent File Date: 10/18/2012

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Howard, John, Egelkrout, Erin, and Hayden, Celine. Method of increasing expression of nucleic acid molecules in plants using multiple transcription units.  United States: N. p., 2019. 
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                    Howard, John, Egelkrout, Erin, & Hayden, Celine. Method of increasing expression of nucleic acid molecules in plants using multiple transcription units.  United States.

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                    Howard, John, Egelkrout, Erin, and Hayden, Celine. Tue .  
        "Method of increasing expression of nucleic acid molecules in plants using multiple transcription units".  United States.  https://www.osti.gov/servlets/purl/1568424.

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@article{osti_1568424,

  title        = {Method of increasing expression of nucleic acid molecules in plants using multiple transcription units},

  author       = {Howard, John and Egelkrout, Erin and Hayden, Celine},

  abstractNote = {A method of increasing expression of a product encoded by a nucleic acid molecule is provided where in one embodiment multiple plant transcription units comprising a promoter and the nucleic acid molecule are provided. In another embodiment multiple plant transcription units are provided where each promoter is different from the other. In an embodiment the multiple plant transcription units may comprise two, three, four or more plant transcription units. In another embodiment the promoter may be selected from an embryo promoter. Another embodiment provides the promoter may be a globulin promoter. A further embodiment provides the product encoded may be selected from hepatitis b, aprotinin, or a cellulase. Still further embodiments provide the product may be selected from the cellulases E1 and CBH1.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2019},

  month        = {6}

}

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Similar Records

Title: Method of increasing expression of nucleic acid molecules in plants using multiple transcription units

Abstract

Citation Formats

Method and apparatus for producing alcohol patent, August 1982

Simultaneous saccharification and fermentation (SSF) using cellobiose fermenting yeast Brettanomyces custersii patent, March 1992

Alcohol and distillers grain recovery process patent, October 1986

Liquefying aqueous starch slurry followed by saccharification with ion exchange resin patent, May 1982

Fermentable sugar from the hydrolysis of starch derived from dry milled cereal grains patent, October 1983

Transgenic plants expressing a cellulase patent, April 2008

Method for liquefaction of cereal grain starch substrate and apparatus therefor patent, November 1999

Monocotyledonous seed expressing exo-1,4B-glucanase patent, October 2013

Production of ethanol and other fermentation products from biomass patent, August 1999

Transgenic plants as an alternative source of lignocellulosic-degrading enzymes patent, November 1999

Globulin 2 regulatory region and method of using same patent, September 2006

Alcohol manufacturing process patent, January 1984

Self-processing plants and plant parts patent, September 2006

Method of continuous treatment of grain mash for producing ethanol patent, November 1983

Embryo preferred promoter and method of using same patent, February 2007

Method for mashing starch-rich material for alcohol production patent, November 1983

Process for the continuous production of ethanol from cereals patent, August 1996

Methods for the cost-effective saccharification of lignocellulosic biomass patent-application, June 2003

Process and apparatus for producing starch-containing feedstock hydrolysates for alcoholic fermentation patent, November 1981

Expression of enzymes involved in cellulose modification patent, January 2000

Regulatory region preferentially expressing to seed embryo and method of using same patent, February 2014

Process of producing ethanol and various other by-products from cereals patent, March 1989

Production of ethanol from biomass patent, October 1997

Process for producing ethanol patent, July 1993

Transgenic plants as an alternative source of lignocellulosic-degrading enzymes patent, November 2004

Fermentable sugar from the hydrolysis of starch derived from dry milled corn patent, September 1981

Process for the production of ethanol patent, August 1990

Methods of saccharification of polysaccharides in plants patent, April 2014

Production of enzymes in seeds and their use patent, August 1996

Globulin-1 promoter from maize and method of using same patent, January 2007

Fermentable sugar from the hydrolysis of starch derived from dry milled cereal grains patent, May 1984

Methods of saccharification of polysaccharides in plants patent, April 2014

Method and apparatus for producing alcohol
patent, August 1982

Simultaneous saccharification and fermentation (SSF) using cellobiose fermenting yeast Brettanomyces custersii
patent, March 1992

Alcohol and distillers grain recovery process
patent, October 1986

Liquefying aqueous starch slurry followed by saccharification with ion exchange resin
patent, May 1982

Fermentable sugar from the hydrolysis of starch derived from dry milled cereal grains
patent, October 1983

Transgenic plants expressing a cellulase
patent, April 2008

Method for liquefaction of cereal grain starch substrate and apparatus therefor
patent, November 1999

Monocotyledonous seed expressing exo-1,4B-glucanase
patent, October 2013

Production of ethanol and other fermentation products from biomass
patent, August 1999

Transgenic plants as an alternative source of lignocellulosic-degrading enzymes
patent, November 1999

Globulin 2 regulatory region and method of using same
patent, September 2006

Alcohol manufacturing process
patent, January 1984

Self-processing plants and plant parts
patent, September 2006

Method of continuous treatment of grain mash for producing ethanol
patent, November 1983

Embryo preferred promoter and method of using same
patent, February 2007

Method for mashing starch-rich material for alcohol production
patent, November 1983

Process for the continuous production of ethanol from cereals
patent, August 1996

Methods for the cost-effective saccharification of lignocellulosic biomass
patent-application, June 2003

Process and apparatus for producing starch-containing feedstock hydrolysates for alcoholic fermentation
patent, November 1981

Expression of enzymes involved in cellulose modification
patent, January 2000

Regulatory region preferentially expressing to seed embryo and method of using same
patent, February 2014

Process of producing ethanol and various other by-products from cereals
patent, March 1989

Production of ethanol from biomass
patent, October 1997

Process for producing ethanol
patent, July 1993

Transgenic plants as an alternative source of lignocellulosic-degrading enzymes
patent, November 2004

Fermentable sugar from the hydrolysis of starch derived from dry milled corn
patent, September 1981

Process for the production of ethanol
patent, August 1990

Methods of saccharification of polysaccharides in plants
patent, April 2014

Production of enzymes in seeds and their use
patent, August 1996

Globulin-1 promoter from maize and method of using same
patent, January 2007

Fermentable sugar from the hydrolysis of starch derived from dry milled cereal grains
patent, May 1984

Methods of saccharification of polysaccharides in plants
patent, April 2014