Method of increasing expression of nucleic acid molecules in plants using multiple transcription units

Title: Method of increasing expression of nucleic acid molecules in plants using multiple transcription units

Abstract

A method of increasing expression of a product encoded by a nucleic acid molecule is provided where in one embodiment multiple plant transcription units comprising a promoter and the nucleic acid molecule are provided. In another embodiment multiple plant transcription units are provided where each promoter is different from the other. In an embodiment the multiple plant transcription units may comprise two, three, four or more plant transcription units. In another embodiment the promoter may be selected from an embryo promoter. Another embodiment provides the promoter may be a globulin promoter. A further embodiment provides the product encoded may be selected from hepatitis b, aprotinin, or a cellulase. Still further embodiments provide the product may be selected from the cellulases E1 and CBH1.

Inventors:: Howard, John; Egelkrout, Erin; Hayden, Celine

Issue Date:: 2019-06-04

Research Org.:: Applied Biotechnology Inst., Inc., San Luis Obispo, CA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1568424

Patent Number(s):: 10,308,948

Application Number:: 13/654,673

Assignee:: Applied Biotechnology Institute, Inc. (San Luis Obispo, CA)

DOE Contract Number:: FG36-08GO88025

Resource Type:: Patent

Resource Relation:: Patent File Date: 10/18/2012

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Howard, John, Egelkrout, Erin, and Hayden, Celine. Method of increasing expression of nucleic acid molecules in plants using multiple transcription units.  United States: N. p., 2019. 
        Web.

Copy to clipboard


                    Howard, John, Egelkrout, Erin, & Hayden, Celine. Method of increasing expression of nucleic acid molecules in plants using multiple transcription units.  United States.

Copy to clipboard


                    Howard, John, Egelkrout, Erin, and Hayden, Celine. Tue .  
        "Method of increasing expression of nucleic acid molecules in plants using multiple transcription units".  United States.  https://www.osti.gov/servlets/purl/1568424.

Copy to clipboard


                    
@article{osti_1568424,

  title        = {Method of increasing expression of nucleic acid molecules in plants using multiple transcription units},

  author       = {Howard, John and Egelkrout, Erin and Hayden, Celine},

  abstractNote = {A method of increasing expression of a product encoded by a nucleic acid molecule is provided where in one embodiment multiple plant transcription units comprising a promoter and the nucleic acid molecule are provided. In another embodiment multiple plant transcription units are provided where each promoter is different from the other. In an embodiment the multiple plant transcription units may comprise two, three, four or more plant transcription units. In another embodiment the promoter may be selected from an embryo promoter. Another embodiment provides the promoter may be a globulin promoter. A further embodiment provides the product encoded may be selected from hepatitis b, aprotinin, or a cellulase. Still further embodiments provide the product may be selected from the cellulases E1 and CBH1.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2019},

  month        = {6}

}

Copy to clipboard

Patent:

View Patent

Save / Share:

Export Metadata

Save to My Library

Works referenced in this record:

Method and apparatus for producing alcohol
patent, August 1982

Lionelle, Joseph E.; Staffa, Jeffrey A.; McCormick, William L.
US Patent Document 4,347,321
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=4347321

Simultaneous saccharification and fermentation (SSF) using cellobiose fermenting yeast Brettanomyces custersii
patent, March 1992

Spindler, Diane D.; Grohmann, Karel; Wyman, Charles E.
US Patent Document 5,100,791
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=5100791

Alcohol and distillers grain recovery process
patent, October 1986

Anderson, Clyde G.; Vara, Tomas E.
US Patent Document 4,617,270
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=4617270

Liquefying aqueous starch slurry followed by saccharification with ion exchange resin
patent, May 1982

Miller, Franklyn D.; Muller, Werner C.
US Patent Document 4,330,625
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=4330625

Fermentable sugar from the hydrolysis of starch derived from dry milled cereal grains
patent, October 1983

Muller, Werner C.; Miller, Franklyn D.
US Patent Document 4,407,955
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=4407955

Transgenic plants expressing a cellulase
patent, April 2008

Lebel, Edouard Guillaume; Heifetz, Peter; Ward, Eric R.
US Patent Document 7,361,806
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=7361806

Method for liquefaction of cereal grain starch substrate and apparatus therefor
patent, November 1999

Meagher, Michael M.; Grafelman, Daryl D.
US Patent Document 5,981,237
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=5981237

Monocotyledonous seed expressing exo-1,4B-glucanase
patent, October 2013

Hood, Elizabeth E.; Howard, John
US Patent Document 8,558,058
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=8558058

Production of ethanol and other fermentation products from biomass
patent, August 1999

Van Draanen, Arlen J.; Mello, Steven M.
US Patent Document 5,932,456
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=5932456

Transgenic plants as an alternative source of lignocellulosic-degrading enzymes
patent, November 1999

Austin-Phillips, Sandra; Burgess, Richard R.; German, Thomas L.
US Patent Document 5,981,835
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=5981835

Globulin 2 regulatory region and method of using same
patent, September 2006

Streatfield, Stephen J.; Love, Robert P.; Bray, Jeff
US Patent Document 7,112,723
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=7112723

Alcohol manufacturing process
patent, January 1984

Neves, Alan M.
US Patent Document 4,425,433
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=4425433

Self-processing plants and plant parts
patent, September 2006

Lanahan, Michael B.; Basu, Shib Sankar; Batie, Christopher J.
US Patent Document 7,102,057
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=7102057

Method of continuous treatment of grain mash for producing ethanol
patent, November 1983

Kretz, Rolf H.
US Patent Document 4,416,989
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=4416989

Embryo preferred promoter and method of using same
patent, February 2007

Streatfield, Stephen J.; Love, Robert P.; Bray, Jeff
US Patent Document 7,183,109
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=7183109

Method for mashing starch-rich material for alcohol production
patent, November 1983

Ronkainen, Pentti P.; Leppanen, Olavi A.; Harju, Kai J.
US Patent Document 4,415,659
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=4415659

Process for the continuous production of ethanol from cereals
patent, August 1996

Zinnamosca, Francesco; Berruti, Massimo
US Patent Document 5,545,543
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=5545543

Methods for the cost-effective saccharification of lignocellulosic biomass
patent-application, June 2003

Hood, Elizabeth E.; Howard, John A.
US Patent Application 10/310292; 20030109011
URL: http://appft.uspto.gov/netacgi/nph-Parser?Sect1=PTO1&Sect2=HITOFF&d=PG01&p=1&u=%2Fnetahtml%2FPTO%2Fsrchnum.html&r=1&f=G&l=50&s1=%2220030109011%22.PGNR.&OS=DN/20030109011&RS=DN/20030109011

Process and apparatus for producing starch-containing feedstock hydrolysates for alcoholic fermentation
patent, November 1981

Benyaev, Negmat E.; Boskanian, Razmik A.; Yarovenko, Viktor I.
US Patent Document 4,302,543
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=4302543

Expression of enzymes involved in cellulose modification
patent, January 2000

Himmel, Michael E.; Schaaf, David J.; Stalker, David M.
US Patent Document 6,013,860
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=6013860

Regulatory region preferentially expressing to seed embryo and method of using same
patent, February 2014

Streatfield, Stephen J.; Love, Robert P.; Bray, Jeff
US Patent Document 8,642,749
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=8642749

Process of producing ethanol and various other by-products from cereals
patent, March 1989

Monceaux, Philippe; Segard, Emile
US Patent Document 4,810,647
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=4810647

Production of ethanol from biomass
patent, October 1997

Van Draanen, Arlen J.; Mello, Steven M.
US Patent Document 5,677,154
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=5677154

Process for producing ethanol
patent, July 1993

Lantero, Jr., Oreste J.; Fish, John J.
US Patent Document 5,231,017
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=5231017

Transgenic plants as an alternative source of lignocellulosic-degrading enzymes
patent, November 2004

Austin-Phillips, Sandra; Burgess, Richard R.; German, Thomas L.
US Patent Document 6,818,803
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=6818803

Fermentable sugar from the hydrolysis of starch derived from dry milled corn
patent, September 1981

Muller, Werner C.; Miller, Franklyn D.
US Patent Document 4,287,304
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=4287304

Process for the production of ethanol
patent, August 1990

Granstedt, Jurgen
US Patent Document 4,952,503
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=4952503

Methods of saccharification of polysaccharides in plants
patent, April 2014

Howard, John; Fake, Gina
US Patent Document 8,709,761
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=8709761

Production of enzymes in seeds and their use
patent, August 1996

Van Ooijen, Albert J.; Rietveld, Krijn; Hoekema, Andreas
US Patent Document 5,543,576
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=5543576

Globulin-1 promoter from maize and method of using same
patent, January 2007

Streatfield, Stephen J.; Love, Robert P.; Bray, Jeff
US Patent Document 7,169,967
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=7169967

Fermentable sugar from the hydrolysis of starch derived from dry milled cereal grains
patent, May 1984

Muller, Werner C.; Miller, Franklyn D.
US Patent Document 4,448,881
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=4448881

Methods of saccharification of polysaccharides in plants
patent, April 2014

Howard, John; Fake, Gina
US Patent Document 8,709,742
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=8709742

Similar records in DOE Patents and OSTI.GOV collections:

Modified SSCP method using sequential electrophoresis of multiple nucleic acid segments

Patent Gatti, Richard A [Sherman Oaks, CA]

The present invention is directed to a method of screening large, complex, polyexonic eukaryotic genes such as the ATM gene for mutations and polymorphisms by an improved version of single strand conformation polymorphism (SSCP) electrophoresis that allows electrophoresis of two or three amplified segments in a single lane. The present invention also is directed to new mutations and polymorphisms in the ATM gene that are useful in performing more accurate screening of human DNA samples for mutations and in distinguishing mutations from polymorphisms, thereby improving the efficiency of automated screening methods.
Full Text Available
Method for sequencing nucleic acid molecules

Patent Korlach, Jonas; Webb, Watt W.; Levene, Michael; ...

The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme on the template nucleic acid molecule to be sequenced is followed in real time. The sequence is deduced by identifying which base is being incorporated into the growing complementary strand of the target nucleic acid by the catalytic activity of the nucleic acid polymerizing enzyme at each step in themore »« less
Full Text Available
Method for sequencing nucleic acid molecules

Patent Korlach, Jonas; Webb, Watt W.; Levene, Michael; ...

The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme on the template nucleic acid molecule to be sequenced is followed in real time. The sequence is deduced by identifying which base is being incorporated into the growing complementary strand of the target nucleic acid by the catalytic activity of the nucleic acid polymerizing enzyme at each step in themore »« less
Full Text Available
Method for sequencing nucleic acid molecules

Patent Korlach, Jonas; Webb, Watt W.; Levene, Michael; ...

The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme on the template nucleic acid molecule to be sequenced is followed in real time. The sequence is deduced by identifying which base is being incorporated into the growing complementary strand of the target nucleic acid by the catalytic activity of the nucleic acid polymerizing enzyme at each step in themore »« less
Full Text Available
Method for nucleic acid hybridization using single-stranded DNA binding protein

Patent Tabor, Stanley [Cambridge, MA]; Richardson, Charles C [Chestnut Hill, MA]

Method of nucleic acid hybridization for detecting the presence of a specific nucleic acid sequence in a population of different nucleic acid sequences using a nucleic acid probe. The nucleic acid probe hybridizes with the specific nucleic acid sequence but not with other nucleic acid sequences in the population. The method includes contacting a sample (potentially including the nucleic acid sequence) with the nucleic acid probe under hybridizing conditions in the presence of a single-stranded DNA binding protein provided in an amount which stimulates renaturation of a dilute solution (i.e., one in which the t.sub.1/2 of renaturation is longer thanmore »« less
Full Text Available

Similar Records

Title: Method of increasing expression of nucleic acid molecules in plants using multiple transcription units

Abstract

Citation Formats

Method and apparatus for producing alcohol patent, August 1982

Simultaneous saccharification and fermentation (SSF) using cellobiose fermenting yeast Brettanomyces custersii patent, March 1992

Alcohol and distillers grain recovery process patent, October 1986

Liquefying aqueous starch slurry followed by saccharification with ion exchange resin patent, May 1982

Fermentable sugar from the hydrolysis of starch derived from dry milled cereal grains patent, October 1983

Transgenic plants expressing a cellulase patent, April 2008

Method for liquefaction of cereal grain starch substrate and apparatus therefor patent, November 1999

Monocotyledonous seed expressing exo-1,4B-glucanase patent, October 2013

Production of ethanol and other fermentation products from biomass patent, August 1999

Transgenic plants as an alternative source of lignocellulosic-degrading enzymes patent, November 1999

Globulin 2 regulatory region and method of using same patent, September 2006

Alcohol manufacturing process patent, January 1984

Self-processing plants and plant parts patent, September 2006

Method of continuous treatment of grain mash for producing ethanol patent, November 1983

Embryo preferred promoter and method of using same patent, February 2007

Method for mashing starch-rich material for alcohol production patent, November 1983

Process for the continuous production of ethanol from cereals patent, August 1996

Methods for the cost-effective saccharification of lignocellulosic biomass patent-application, June 2003

Process and apparatus for producing starch-containing feedstock hydrolysates for alcoholic fermentation patent, November 1981

Expression of enzymes involved in cellulose modification patent, January 2000

Regulatory region preferentially expressing to seed embryo and method of using same patent, February 2014

Process of producing ethanol and various other by-products from cereals patent, March 1989

Production of ethanol from biomass patent, October 1997

Process for producing ethanol patent, July 1993

Transgenic plants as an alternative source of lignocellulosic-degrading enzymes patent, November 2004

Fermentable sugar from the hydrolysis of starch derived from dry milled corn patent, September 1981

Process for the production of ethanol patent, August 1990

Methods of saccharification of polysaccharides in plants patent, April 2014

Production of enzymes in seeds and their use patent, August 1996

Globulin-1 promoter from maize and method of using same patent, January 2007

Fermentable sugar from the hydrolysis of starch derived from dry milled cereal grains patent, May 1984

Methods of saccharification of polysaccharides in plants patent, April 2014

Method and apparatus for producing alcohol
patent, August 1982

Simultaneous saccharification and fermentation (SSF) using cellobiose fermenting yeast Brettanomyces custersii
patent, March 1992

Alcohol and distillers grain recovery process
patent, October 1986

Liquefying aqueous starch slurry followed by saccharification with ion exchange resin
patent, May 1982

Fermentable sugar from the hydrolysis of starch derived from dry milled cereal grains
patent, October 1983

Transgenic plants expressing a cellulase
patent, April 2008

Method for liquefaction of cereal grain starch substrate and apparatus therefor
patent, November 1999

Monocotyledonous seed expressing exo-1,4B-glucanase
patent, October 2013

Production of ethanol and other fermentation products from biomass
patent, August 1999

Transgenic plants as an alternative source of lignocellulosic-degrading enzymes
patent, November 1999

Globulin 2 regulatory region and method of using same
patent, September 2006

Alcohol manufacturing process
patent, January 1984

Self-processing plants and plant parts
patent, September 2006

Method of continuous treatment of grain mash for producing ethanol
patent, November 1983

Embryo preferred promoter and method of using same
patent, February 2007

Method for mashing starch-rich material for alcohol production
patent, November 1983

Process for the continuous production of ethanol from cereals
patent, August 1996

Methods for the cost-effective saccharification of lignocellulosic biomass
patent-application, June 2003

Process and apparatus for producing starch-containing feedstock hydrolysates for alcoholic fermentation
patent, November 1981

Expression of enzymes involved in cellulose modification
patent, January 2000

Regulatory region preferentially expressing to seed embryo and method of using same
patent, February 2014

Process of producing ethanol and various other by-products from cereals
patent, March 1989

Production of ethanol from biomass
patent, October 1997

Process for producing ethanol
patent, July 1993

Transgenic plants as an alternative source of lignocellulosic-degrading enzymes
patent, November 2004

Fermentable sugar from the hydrolysis of starch derived from dry milled corn
patent, September 1981

Process for the production of ethanol
patent, August 1990

Methods of saccharification of polysaccharides in plants
patent, April 2014

Production of enzymes in seeds and their use
patent, August 1996

Globulin-1 promoter from maize and method of using same
patent, January 2007

Fermentable sugar from the hydrolysis of starch derived from dry milled cereal grains
patent, May 1984

Methods of saccharification of polysaccharides in plants
patent, April 2014