Regioselective alkane hydroxylation with a mutant AlkB enzyme
Abstract
AlkB from Pseudomonas putida was engineered using in-vivo directed evolution to hydroxylate small chain alkanes. Mutant AlkB-BMO1 hydroxylates propane and butane at the terminal carbon at a rate greater than the wild-type to form 1-propanol and 1-butanol, respectively. Mutant AlkB-BMO2 similarly hydroxylates propane and butane at the terminal carbon at a rate greater than the wild-type to form 1-propanol and 1-butanol, respectively. These biocatalysts are highly active for small chain alkane substrates and their regioselectivity is retained in whole-cell biotransformations.
- Inventors:
- Issue Date:
- Research Org.:
- California Institute of Technology (CalTech), Pasadena, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1080415
- Patent Number(s):
- 8309333
- Application Number:
- 12/618,960
- Assignee:
- The United States of America, as represented by Department of Energy (Washington, DC)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- FG02-06ER15762
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2009 Nov 16
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Koch, Daniel J., and Arnold, Frances H. Regioselective alkane hydroxylation with a mutant AlkB enzyme. United States: N. p., 2012.
Web.
Koch, Daniel J., & Arnold, Frances H. Regioselective alkane hydroxylation with a mutant AlkB enzyme. United States.
Koch, Daniel J., and Arnold, Frances H. Tue .
"Regioselective alkane hydroxylation with a mutant AlkB enzyme". United States. https://www.osti.gov/servlets/purl/1080415.
@article{osti_1080415,
title = {Regioselective alkane hydroxylation with a mutant AlkB enzyme},
author = {Koch, Daniel J. and Arnold, Frances H.},
abstractNote = {AlkB from Pseudomonas putida was engineered using in-vivo directed evolution to hydroxylate small chain alkanes. Mutant AlkB-BMO1 hydroxylates propane and butane at the terminal carbon at a rate greater than the wild-type to form 1-propanol and 1-butanol, respectively. Mutant AlkB-BMO2 similarly hydroxylates propane and butane at the terminal carbon at a rate greater than the wild-type to form 1-propanol and 1-butanol, respectively. These biocatalysts are highly active for small chain alkane substrates and their regioselectivity is retained in whole-cell biotransformations.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Nov 13 00:00:00 EST 2012},
month = {Tue Nov 13 00:00:00 EST 2012}
}
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